Anti-PCBP2/hnRNP E2 抗体 [EPR14859(2)] (ab200835)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14859(2)] to PCBP2/hnRNP E2
- Suitable for: Flow Cyt (Intra), IP, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-PCBP2/hnRNP E2 antibody [EPR14859(2)]
PCBP2/hnRNP E2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14859(2)] to PCBP2/hnRNP E2 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IP, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: HeLa, Jurkat, K562, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse brain, Mouse heart, Rat heart and Rat spleen lysates. ICC/IF: HeLa cells. Flow Cyt (intra): Jurkat cells. IP: Jurkat whole cell lysate.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR14859(2) -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab200835の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/250.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IP |
1/80.
|
|
WB |
1/1000. Detects a band of approximately 35, 39 kDa (predicted molecular weight: 34-39 kDa).
|
|
ICC/IF |
1/500.
|
特記事項 |
---|
Flow Cyt (Intra)
1/250. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
1/80. |
WB
1/1000. Detects a band of approximately 35, 39 kDa (predicted molecular weight: 34-39 kDa). |
ICC/IF
1/500. |
ターゲット情報
-
機能
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation. -
組織特異性
Detected in all tissues examined. -
配列類似性
Contains 3 KH domains. -
翻訳後修飾
Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity. -
細胞内局在
Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 5094 Human
- Entrez Gene: 18521 Mouse
- Entrez Gene: 363005 Rat
- Omim: 601210 Human
- SwissProt: Q15366 Human
- SwissProt: Q61990 Mouse
- Unigene: 546271 Human
- Unigene: 236513 Mouse
-
別名
- Alpha CP2 antibody
- Alpha-CP2 antibody
- alphaCP-2 antibody
see all
画像
-
All lanes : Anti-PCBP2/hnRNP E2 antibody [EPR14859(2)] (ab200835) at 1/10000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34-39 kDa
Observed band size: 35,39 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBased on sequence analysis, ab200835 recognizes isoforms 1-6 which have corresponding Mw between 34-39kDa.
Blocking/Dilution Buffer: 5% NFDM/TBST.
-
All lanes : Anti-PCBP2/hnRNP E2 antibody [EPR14859(2)] (ab200835) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat heart lysate
Lane 4 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34-39 kDa
Observed band size: 35,39 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution Buffer: 5% NFDM/TBST.
-
All lanes : Anti-PCBP2/hnRNP E2 antibody [EPR14859(2)] (ab200835) at 1/1000 dilution
Lane 1 : Rat heart lysate
Lane 2 : C6 (Rat glial tumor cells) whole cell lysate
Lane 3 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34-39 kDa
Observed band size: 35,39 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution Buffer: 5% NFDM/TBST.
-
All lanes : Anti-PCBP2/hnRNP E2 antibody [EPR14859(2)] (ab200835) at 1/10000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with PCBP2/hnRNP E2 peptide
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with PCBP1 peptide
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34-39 kDa
Observed band size: 35,39 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution Buffer: 5% NFDM/TBST.
Based on sequence analysis, ab200835 (PCBP2/hnRNP E2) shares 80% homology with family member PCBP1. The levels of XR were tested in the accompanying blocking experiment.
-
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling PCBP2/hnRNP E2 with ab200835 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear and cytoplasmic staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1: ab200835 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling PCBP2/hnRNP E2 with ab200835 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
-
PCBP2/hnRNP E2 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200835 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab200835 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Jurkat whole cell lysate 10 µg (Input). Lane 2: ab200835 IP in Jurkat whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200835 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 5 seconds
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab200835 は論文での使用が確認できていません。