保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファーPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab74793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use a concentration of 1 mg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.|
機能Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.
組織特異性Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
配列類似性Contains 3 KH domains.
翻訳後修飾Phosphorylated; lowers poly(rC)-binding activity.
細胞内局在Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
- Information by UniProt
- Alpha-CP1 antibody
- Heterogeneous nuclear ribonucleoprotein E1 antibody
- heterogenous nuclear ribonucleoprotein E1 antibody
Western blot analysis of Jurkat cell lysate labeling PCBP1 with ab74793 at 1.0µg/ml.
Western Blot using ab74793 at 1.0ug/ml. Sample type: Human MCF7 Lysate
ab74793 staining PCBP1 in Human breast Tissue by Immunohistochemistry (Paraffin-embedded sections). Primary antibody used at 5 μg/ml
ab74793 staining PCBP1 in Human spleen Tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) after heat treatment antigen retrieval. Primary antibody used at 5 μg/ml
Anti-PCBP1 antibody (ab74793) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
ICC/IF image of ab74793 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74793, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab74793 at 5µg/ml staining PCBP1 in Human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue).
All lanes : Anti-PCBP1 antibody (ab74793) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from MEF cells
Lane 2 : Whole cell lysate prepared from F9 cells
Lane 3 : Whole cell lysate prepared from GC1 cells
Lane 4 : Whole cell lysate prepared from NIH-3T3 cells
Lysates/proteins at 30 µg per lane.
All lanes : HRP conjugated pig anti-rabbit polyclonal
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
This product has been referenced in:
- Emde A et al. Dysregulated miRNA biogenesis downstream of cellular stress and ALS-causing mutations: a new mechanism for ALS. EMBO J 34:2633-51 (2015). Read more (PubMed: 26330466) »
- Ho JJ et al. Active stabilization of human endothelial nitric oxide synthase mRNA by hnRNP E1 protects against antisense RNA and microRNAs. Mol Cell Biol 33:2029-46 (2013). Human . Read more (PubMed: 23478261) »