製品の概要
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製品名Anti-PC1/3 antibody
PC1/3 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to PC1/3
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由来種Rabbit
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アプリケーション適用あり: IP, IHC-P, WBmore details
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種交差性交差種: Mouse
交差が予測される動物種: Rat, Cow, Human
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免疫原
Synthetic peptide corresponding to Mouse PC1/3 aa 111-127.
Sequence:SVQKDSALDLFNDPMWN
(Peptide available asab5011) -
ポジティブ･コントロール
- WB: mouse pituitary extract IHC-P: FFPE mouse small intestine tissue sections.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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一次抗体 備考The subtilisin-like Prohormone Convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab3532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.4 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 80 kDa).Can be blocked with PC1/3 peptide (ab5011). |
ターゲット情報
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機能Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.
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関連疾患Defects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency) [MIM:600955]. PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction It is due to impaired processing of prohormones.
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配列類似性Belongs to the peptidase S8 family. Furin subfamily.
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細胞内局在Cytoplasmic vesicle > secretory vesicle. Localized in the secretion granules.
- Information by UniProt
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参照データベース
- Entrez Gene: 281967 Cow
- Entrez Gene: 5122 Human
- Entrez Gene: 18548 Mouse
- Entrez Gene: 25204 Rat
- Omim: 162150 Human
- SwissProt: Q9GLR1 Cow
- SwissProt: P29120 Human
- SwissProt: P63239 Mouse
see all -
別名
- BDP antibody
- NEC 1 antibody
- NEC1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody (ab3532)IHC image of PC1/3 staining in Mouse small intestine formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3532, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DAB. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-PC1/3 antibody (ab3532)Western blot detection of PC1/3 from mouse pituitary extract using ab3532. -
Western blot - Anti-PC1/3 antibody (ab3532)Anti-PC1/3 antibody (ab3532) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 87 + 68 kDa (why is the actual band size different from the predicted?)
Additional bands at: 35 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
PC1/3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The band observed at 68 kDa could potentially be a cleaved form of PC1/3 due to the presence of a 27 amino acid signal peptide and an 83 amino acid propeptide.
参考文献
This product has been referenced in:
- Irwin N et al. Chemical cholecystokinin receptor activation protects against obesity-diabetes in high fat fed mice and has sustainable beneficial effects in genetic ob/ob mice. Biochem Pharmacol : (2012). Read more (PubMed: 23085436) »
- Gagnon J et al. Expression of PCSK1 (PC1/3), PCSK2 (PC2) and PCSK3 (furin) in mouse small intestine. Regul Pept 152:54-60 (2009). Read more (PubMed: 18706454) »
レビューと Q&A
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