Key features and details
- Rabbit polyclonal to PC1/3
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
PC1/3 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to PC1/3
アプリケーション適用あり: WBmore details
交差が予測される動物種: Rat, Cow, Human
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Concentration information loading...
精製度Immunogen affinity purified
一次抗体 備考The subtilisin-like Prohormone Convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm.
Our Abpromise guarantee covers the use of ab3532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.4 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 80 kDa).|
機能Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.
関連疾患Defects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency) [MIM:600955]. PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction It is due to impaired processing of prohormones.
配列類似性Belongs to the peptidase S8 family. Furin subfamily.
細胞内局在Cytoplasmic vesicle > secretory vesicle. Localized in the secretion granules.
- Information by UniProt
- BDP antibody
- NEC 1 antibody
- NEC1 antibody
Western blot detection of PC1/3 from mouse pituitary extract using ab3532.
Anti-PC1/3 antibody (ab3532) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 87 + 68 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
PC1/3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The band observed at 68 kDa could potentially be a cleaved form of PC1/3 due to the presence of a 27 amino acid signal peptide and an 83 amino acid propeptide.
ab3532 は 2 報の論文で使用されています。
- Irwin N et al. Chemical cholecystokinin receptor activation protects against obesity-diabetes in high fat fed mice and has sustainable beneficial effects in genetic ob/ob mice. Biochem Pharmacol : (2012). PubMed: 23085436
- Gagnon J et al. Expression of PCSK1 (PC1/3), PCSK2 (PC2) and PCSK3 (furin) in mouse small intestine. Regul Pept 152:54-60 (2009). PubMed: 18706454