Anti-PBX1/PRL 抗体 (ab97994)
Key features and details
- Rabbit polyclonal to PBX1/PRL
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PBX1/PRL antibody
PBX1/PRL 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PBX1/PRL -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken, Zebrafish
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HL60; K562; MOLT4.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97994の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa). |
ターゲット情報
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機能
Binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development and differentiation. -
組織特異性
Expressed in all tissues except in cells of the B and T lineage. -
関連疾患
Note=A chromosomal aberration involving PBX1 is a cause of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with TCF3. E2A-PBX1 transforms cells by constitutively activating transcription of genes regulated by PBX1 or by other members of the PBX protein family. -
配列類似性
Belongs to the TALE/PBX homeobox family.
Contains 1 homeobox DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 395505 Chicken
- Entrez Gene: 5087 Human
- Entrez Gene: 18514 Mouse
- Entrez Gene: 304947 Rat
- Omim: 176310 Human
- SwissProt: P40424 Human
- SwissProt: P41778 Mouse
- Unigene: 557097 Human
see all -
別名
- DKFZp686B09108 antibody
- Homeo box protein PBX1 antibody
- Homeo box protein PRL antibody
see all
画像
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Western blot - Anti-PBX1/PRL antibody (ab97994)All lanes : Anti-PBX1/PRL antibody (ab97994) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 5 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with immunizing peptide at 1 µg/ml
Lane 7 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with immunizing peptide at 1 µg/ml
Lane 8 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate with immunizing peptide at 1 µg/ml
Lane 9 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate with immunizing peptide at 1 µg/ml
Lane 10 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate with immunizing peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-PBX1/PRL antibody (ab97994)ICC/IF image of ab97994 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97994, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 and Hek392 cells at 5µg/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (4)
ab97994 は 4 報の論文で使用されています。
- Liu N et al. MicroRNA-181 regulates the development of Ossification of Posterior longitudinal ligament via Epigenetic Modulation by targeting PBX1. Theranostics 10:7492-7509 (2020). PubMed: 32685001
- Li J et al. Dynamic changes in cis-regulatory occupancy by Six1 and its cooperative interactions with distinct cofactors drive lineage-specific gene expression programs during progressive differentiation of the auditory sensory epithelium. Nucleic Acids Res 48:2880-2896 (2020). PubMed: 31956913
- Zhang LX et al. uc.38 induces breast cancer cell apoptosis via PBX1. Am J Cancer Res 7:2438-2451 (2017). PubMed: 29312798
- Clarke-Harris R et al. PGC1a promoter methylation in blood at 5-7 years predicts adiposity from 9 to 14 years (EarlyBird 50). Diabetes 63:2528-37 (2014). Human . PubMed: 24622795
