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遺伝子ノックアウト細胞株 検証済リコンビナント

Anti-PBK/SPK 抗体 [31/PBK] (ab280209)

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Western blot - Anti-PBK/SPK antibody [31/PBK] (ab280209)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [31/PBK] (ab280209)
  • Flow Cytometry (Intracellular) - Anti-PBK/SPK antibody [31/PBK] (ab280209)
  • Western blot - Anti-PBK/SPK antibody [31/PBK] (ab280209)
  • Western blot - Anti-PBK/SPK antibody [31/PBK] (ab280209)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [31/PBK] (ab280209)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [31/PBK] (ab280209)
  • Anti-PBK/SPK antibody [31/PBK] (ab280209)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [31/PBK] to PBK/SPK
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PBK/SPK antibody [31/PBK]
    PBK/SPK 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [31/PBK] to PBK/SPK

  • 由来種

    Mouse

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-Pmore details
    適用なし: ICC

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Wild-type HEK-293T whole cell lysate. HeLa, HepG2, RAW264.7, C6, PC-12 and U-87 MG whole cell lysate. Human, rat and mouse testis tissue lysate. IHC-P: Human tonsil tissue. Mouse and rat testis tissue. Flow Cyt (intra) (intra): HEK-239T cells.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab280209の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/1000.
WB
1/1000. Predicted molecular weight: 36 kDa.
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/1000.
WB
1/1000. Predicted molecular weight: 36 kDa.
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for ICC.

ターゲット情報

  • 機能

    Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.

  • 組織特異性

    Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.

  • 配列類似性

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Phosphorylated; in a cell-cycle dependent manner at mitosis.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Cancer/testis antigen 84 antibody
      • CT84 antibody
      • Epididymis luminal protein 164 antibody
      • FLJ14385 antibody
      • HEL164 antibody
      • Lymphokine activated killer T cell originated protein kinase antibody
      • Lymphokine-activated killer T-cell-originated protein kinase antibody
      • MAPKK like protein kinase antibody
      • MAPKK-like protein kinase antibody
      • Nori 3 antibody
      • Nori-3 antibody
      • Nori3 antibody
      • PBK antibody
      • PDZ binding kinase antibody
      • PDZ-binding kinase antibody
      • Serine/threonine protein kinase antibody
      • Spermatogenesis related protein kinase antibody
      • Spermatogenesis-related protein kinase antibody
      • SPK antibody
      • T LAK cell originated protein kinase antibody
      • T-LAK cell-originated protein kinase antibody
      • TOPK antibody
      • TOPK_HUMAN antibody
      see all

    画像

    • Western blot - Anti-PBK/SPK antibody [31/PBK] (ab280209)
      Western blot - Anti-PBK/SPK antibody [31/PBK] (ab280209)
      All lanes : Anti-PBK/SPK antibody [31/PBK] (ab280209) at 1/1000 dilution

      Lane 1 : Wild-type HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
      Lane 2 : PBK knockout HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
      Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
      Lane 4 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG H&L (IRDye® 800CW) (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (ab216777) at 1/10000 dilution

      Predicted band size: 36 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab280209 observed at 36 kDa. Red - loading control ab52901(Rabbit monoclonal [EP1331Y] to beta Tubulin) observed at 52 kDa.

      Lanes 1-2: ab280209 Anti-PBK antibody was shown to react with in HEK-293T cells in Western blot. Loss of signal was observed when PBK knockout sample was used. Wild-type and PBK knockout samples were subjected to SDS-PAGE.

      ab280209 and Anti-beta Tubulin antibody [EP1331Y] (ab52901) were incubated at 4° overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680CW) (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800RD) (ab216772) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

      Blocking/Dilution buffer: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [31/PBK] (ab280209)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [31/PBK] (ab280209)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PBK/PSK with ab280209 at 1/500 (1.946 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Positive staining in germinal center of human tonsil.

      The section was incubated with ab280209 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    • Flow Cytometry (Intracellular) - Anti-PBK/SPK antibody [31/PBK] (ab280209)
      Flow Cytometry (Intracellular) - Anti-PBK/SPK antibody [31/PBK] (ab280209)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling PBK/SPK with ab280209 at 1/1000 dilution (0.1 µg) (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

    • Western blot - Anti-PBK/SPK antibody [31/PBK] (ab280209)
      Western blot - Anti-PBK/SPK antibody [31/PBK] (ab280209)
      All lanes : Anti-PBK/SPK antibody [31/PBK] (ab280209) at 1/1000 dilution

      Lane 1 : Human testis tissue lysate
      Lane 2 : Human heart tissue lysate
      Lane 3 : Mouse testis tissue lysate
      Lane 4 : Mouse brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

      Predicted band size: 36 kDa



      Negative control: Human heart, mouse brain (PMID: 27898655).

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:23547718).

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-PBK/SPK antibody [31/PBK] (ab280209)
      Western blot - Anti-PBK/SPK antibody [31/PBK] (ab280209)
      All lanes : Anti-PBK/SPK antibody [31/PBK] (ab280209) at 1/1000 dilution

      Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
      Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
      Lane 4 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
      Lane 5 : C6 (rat glial tumor glial cell), whole cell lysate
      Lane 6 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
      Lane 7 : Rat testis tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

      Predicted band size: 36 kDa



      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:23547718).

      Exposure time: lane 1: 37 secs; lane 2-7: 15 secs.

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [31/PBK] (ab280209)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [31/PBK] (ab280209)

      Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling PBK/PSK with ab280209 at 1/500 (1.946 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Positive staining in mouse testis.

      The section was incubated with ab280209 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [31/PBK] (ab280209)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [31/PBK] (ab280209)

      Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling PBK/PSK with ab280209 at 1/500 (1.946 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Positive staining in rat testis.

      The section was incubated with ab280209 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    • Anti-PBK/SPK antibody [31/PBK] (ab280209)
      Anti-PBK/SPK antibody [31/PBK] (ab280209)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab280209 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab280209 は論文での使用が確認できていません。

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