Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1903] to Paxillin (phospho Y118) - BSA and Azide free
- Suitable for: Dot blot, WB
- Reacts with: Mouse, Rat, Human
製品名Anti-Paxillin (phospho Y118) antibody [EPR1903] - BSA and Azide free
Paxillin 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR1903] to Paxillin (phospho Y118) - BSA and Azide free
The antibody only detects Paxillin phosphorylated on Tyrosine 118.
アプリケーション適用あり: Dot blot, WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human Paxillin (phospho Y118). The exact sequence is proprietary.
Ab247906 is the carrier-free version of ab109547. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247906 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab247906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 65 kDa.|
機能Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
配列類似性Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains.
翻訳後修飾Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
細胞内局在Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
- Information by UniProt
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
All lanes : Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with pervanadate
Lysates/proteins at 10 µg per lane.
Predicted band size: 65 kDa
This data was developed using ab109547, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.