Anti-Paxillin (phospho Y118) 抗体 (ab4833)
Key features and details
- Rabbit polyclonal to Paxillin (phospho Y118)
- Suitable for: WB
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG
製品の概要
-
製品名
Anti-Paxillin (phospho Y118) antibody
Paxillin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Paxillin (phospho Y118) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human, African green monkey
交差が予測される動物種: Chicken
-
免疫原
Synthetic peptide (Human) derived from the region of human paxillin that contains tyrosine 118.
-
ポジティブ･コントロール
- WB: COS-7, A431, HeLa whole cell lysates
-
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin, and ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at tyrosine 31. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab4833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 68 kDa. |
ターゲット情報
-
機能
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). -
配列類似性
Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains. -
翻訳後修飾
Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. -
細胞内局在
Cytoplasm > cytoskeleton. Cell junction > focal adhesion. - Information by UniProt
-
参照データベース
- Entrez Gene: 395832 Chicken
- Entrez Gene: 5829 Human
- Entrez Gene: 19303 Mouse
- Omim: 602505 Human
- SwissProt: P49024 Chicken
- SwissProt: P49023 Human
- SwissProt: Q8VI36 Mouse
- Unigene: 446336 Human
see all -
別名
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
see all
画像
-
Western blot - Anti-Paxillin (phospho Y118) antibody (ab4833)All lanes : Anti-Paxillin (phospho Y118) antibody (ab4833) at 1/500 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 2 : COS-7 whole cell lysate treated with 100 uM Pervanadate for 1 hour
Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 4 : A431 whole cell lysate treated with 100 uM Pervanadate for 1 hour
Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 6 : HeLa whole cell lysate treated with 100 uM Pervanadate for 1 hour
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/5000 dilution
Predicted band size: 68 kDaA 66 kDa band corresponding to Paxillin pTyr118 was enhanced across treated cell lines. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.
-
Western blot - Anti-Paxillin (phospho Y118) antibody (ab4833)All lanes : Anti-Paxillin (phospho Y118) antibody (ab4833) at 0.75 µg/ml (3% BSA-TBST buffer)
All lanes : NMµMG (mouse mammary) cells transfected with EGFP-tagged paxillin
Secondary
All lanes : goat F(ab’)2 anti-rabbit IgG alkaline phosphatase
Predicted band size: 68 kDaPeptide Competition: Extracts were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC.
Prior incubation with:
1- no peptide,
2- the non-phosphopeptide corresponding to the immunogen
3- a generic phosphotyrosine containing peptide
4- the phosphopeptide immunogen
Bands were detected using the Tropix WesternStar method.
The data show that only the peptide corresponding to ab4833 blocks the antibody signal, thereby demonstrating the specificity of the antibody.
プロトコール
参考文献 (5)
ab4833 は 5 報の論文で使用されています。
- Schimmel L et al. c-Src controls stability of sprouting blood vessels in the developing retina independently of cell-cell adhesion through focal adhesion assembly. Development 147:N/A (2020). PubMed: 32108024
- Wang H et al. Inhibition of tumor vasculogenic mimicry and prolongation of host survival in highly aggressive gallbladder cancers by norcantharidin via blocking the ephrin type a receptor 2/focal adhesion kinase/paxillin signaling pathway. PLoS One 9:e96982 (2014). WB, ICC/IF ; Mouse . PubMed: 24811250
- Lange K et al. Combined lysophosphatidic acid/platelet-derived growth factor signaling triggers glioma cell migration in a tenascin-C microenvironment. Cancer Res 68:6942-52 (2008). WB ; Human . PubMed: 18757408
- Demo SD et al. Antitumor activity of PR-171, a novel irreversible inhibitor of the proteasome. Cancer Res 67:6383-91 (2007). PubMed: 17616698
- Lange K et al. Endothelin receptor type B counteracts tenascin-C-induced endothelin receptor type A-dependent focal adhesion and actin stress fiber disorganization. Cancer Res 67:6163-73 (2007). WB ; Human . PubMed: 17616673
