Anti-Paxillin 抗体 (ab2264)
製品の概要
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製品名
Anti-Paxillin antibody
Paxillin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Paxillin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Fr, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken
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免疫原
Synthetic peptide corresponding to Mouse Paxillin aa 1-100.
(Peptide available asab13754) -
ポジティブ･コントロール
- This antibody gave a positive signal in NIH3T3 whole cell lysate.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab2264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 68 kDa. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 22629443 | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | 1/100. |
ターゲット情報
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機能
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). -
配列類似性
Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains. -
翻訳後修飾
Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. -
細胞内局在
Cytoplasm > cytoskeleton. Cell junction > focal adhesion. - Information by UniProt
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参照データベース
- Entrez Gene: 395832 Chicken
- Entrez Gene: 5829 Human
- Entrez Gene: 19303 Mouse
- Entrez Gene: 360820 Rat
- Omim: 602505 Human
- SwissProt: P49024 Chicken
- SwissProt: P49023 Human
- SwissProt: Q8VI36 Mouse
see all -
別名
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody (ab2264)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.ab2264 staining human U2OS, mouse MEF, and rat RIE1 cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton-100 [PBS] prior to blocking in 1% BSA for 15 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C. The secondary antibody was an Alexa Fluor® 647 conjugated donkey anti-rabbit, diluted 1/1000.
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Western blot - Anti-Paxillin antibody (ab2264)Anti-Paxillin antibody (ab2264) at 1/500 dilution + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
ab2264 detects a band at 68 kDa in NIH 3T3 lysate, as well as an additional band at 43kDa. An NCBI BLAST search of the peptide sequence identified an N terminal fragment of the Paxillin molecule with a primary molecular weight of 36 kDa. We believe the 43 kDa band observed may correspond to this fragment. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Paxillin antibody (ab2264)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.ab2264 staining human and mouse pancreas and rat testis tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in sequential (prediluted) peroxidase and protein block for 20 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 45 minutes at 20°C. A HRP-conjugated goat anti-rabbit anitbody was used as the secondary.
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Immunohistochemistry (Frozen sections) - Anti-Paxillin antibody (ab2264)Image from Phillips HM et al., PLoS One. 2012;7(5):e37685. Epub 2012 May 21. Fig 8.; doi:10.1371/journal.pone.0037685; May 21, 2012, PLoS ONE 7(5): e37685.Immunohistochemical analysis of frozen sections of frontonasal processes from wild-type (control) or RockDN;Wnt1-cre mutant (RockDN) mouse embyros; Paxillin was stained with ab2264.
プロトコール
参考文献
This product has been referenced in:
- Neganova I et al. Endothelial Differentiation G Protein-Coupled Receptor 5 Plays an Important Role in Induction and Maintenance of Pluripotency. Stem Cells 37:318-331 (2019). Read more (PubMed: 30512203) »
- Luan L et al. HOXD10 silencing suppresses human fibroblast-like synoviocyte migration in rheumatoid arthritis via downregulation of the p38/JNK pathway. Exp Ther Med 16:1621-1628 (2018). Read more (PubMed: 30186380) »
