Anti-PAX8 抗体 (ab13611)
Key features and details
- Goat polyclonal to PAX8
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PAX8 antibody
PAX8 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to PAX8 -
由来種
Goat -
特異性
This antibody is expected to recognise all five human isoforms (as represented by NP_003457; NP_039245; NP_039246; NP_039247; NP_054698). This antibody detects a strong band at approx 40kDa in Human Liver lysates. The identity of this band is unclear given the existence of 5 isoforms of PAX8 between 450 aa and 287 aa (54-34 kD). It has shown to be specific for PAX8 in experiments with nuclear extracts of PC Cl3 thyroid cells cultured for 7 days without stimulation from serum and thyrotropin (starved cells) and then stimulated with thyrotropin (TSH) for 24 h (TSH stimulated cells). A band representing PAX8 is seen in the stimulated cells at 60kD (although other non-specific background bands are also seen). We have no confirmation of which isoform this represents. -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Dog
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免疫原
Synthetic peptide corresponding to Human PAX8 aa 1-100 (N terminal).
Database link: Q06710 -
ポジティブ･コントロール
- IHC-P: Human thyroid tissue WB: Human kidney tissue lysate, HeLa cell lysate and Human thyroid cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13611の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 2 - 4 µg/ml.
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| WB | (1) |
Use a concentration of 1.5 - 4.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 48 kDa).
A 1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
IHC-P
Use a concentration of 2 - 4 µg/ml. |
|
WB
Use a concentration of 1.5 - 4.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 48 kDa). A 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells. -
組織特異性
Expressed in the excretory system, thyroid gland and Wilms tumors. -
関連疾患
Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue. -
配列類似性
Contains 1 paired domain. -
発生段階
In developing excretory system, during thyroid differentiation and in adult thyroid. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 403927 Dog
- Entrez Gene: 7849 Human
- Entrez Gene: 18510 Mouse
- Entrez Gene: 81819 Rat
- Omim: 167415 Human
- SwissProt: Q06710 Human
- SwissProt: Q00288 Mouse
- SwissProt: P51974 Rat
see all -
別名
- OTTHUMP00000158659 antibody
- OTTHUMP00000158660 antibody
- OTTHUMP00000203723 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab13611)
ab13611 (2µg/ml) staining of paraffin embedded Human Human Thyroid Gland following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining shows nuclear stains in the epithelial cells around the follicles.
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Western blot - Anti-PAX8 antibody (ab13611)All lanes : Anti-PAX8 antibody (ab13611) at 1 µg/ml
Lane 1 : Human Kidney tissue lysate (35µg protein in RIPA buffer).
Lane 2 : HeLa cell lysate (35µg protein in RIPA buffer).
Predicted band size: 48 kDaDetected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab13611)ab13611 at 3µg/ml staining PAX8 in human thyroid gland sections by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by microwave in Tris/EDTA buffer pH 9.0. The HRP-staining procedure was used for detection.
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Western blot - Anti-PAX8 antibody (ab13611)Nuclear extract of PC Cl3 thyroid cells (15
µ PAX8 is seen to be correctly regulated in panel A. Panel B shows an extended view of the same gel with other visible background bands.
The indicated PAX8 band is at 60 kD. (In some gels PAX8 runs at 55-58kD).
This image was kindly supplied as part of the review submitted by Dr Pilar Santisteban.
Nuclear extract of PC Cl3 thyroid cells (15 µg) cultured during 7 days without stimulation from serum and thyrotropin (starved cells) and then stimulated with thyrotropin (TSH) 0.5 mUI/ml during 24 h (TSH stimulated cells). PAX8 is seen to be correctly regulated in panel A. Panel B shows an extended view of the same gel with other visible background bands. The indicated PAX8 band is at
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab13611 は 8 報の論文で使用されています。
- Fan H et al. Epigenomic Reprogramming toward Mesenchymal-Epithelial Transition in Ovarian-Cancer-Associated Mesenchymal Stem Cells Drives Metastasis. Cell Rep 33:108473 (2020). PubMed: 33296650
- O'Brien LL et al. Transcriptional regulatory control of mammalian nephron progenitors revealed by multi-factor cistromic analysis and genetic studies. PLoS Genet 14:e1007181 (2018). PubMed: 29377931
- Arauchi A et al. Functional Thyroid Follicular Cells Differentiation from Human-Induced Pluripotent Stem Cells in Suspension Culture. Front Endocrinol (Lausanne) 8:103 (2017). PubMed: 28588551
- Zane M et al. Estrogen and thyroid cancer is a stem affair: A preliminary study. Biomed Pharmacother 85:399-411 (2017). Flow Cyt, ICC/IF . PubMed: 27899250
- Kang HM et al. Sox9-Positive Progenitor Cells Play a Key Role in Renal Tubule Epithelial Regeneration in Mice. Cell Rep 14:861-871 (2016). PubMed: 26776520
- Sicking EM et al. Subtotal ablation of parietal epithelial cells induces crescent formation. J Am Soc Nephrol 23:629-40 (2012). IHC-P ; Mouse . PubMed: 22282596
- Borgogni E et al. Elocalcitol inhibits inflammatory responses in human thyroid cells and T cells. Endocrinology 149:3626-34 (2008). PubMed: 18372324
- Crescioli C et al. Methimazole inhibits CXC chemokine ligand 10 secretion in human thyrocytes. J Endocrinol 195:145-55 (2007). PubMed: 17911406