Application
Western blot
Sample
Mouse Cell lysate - whole cell (neurospheres)
Loading amount
200 µg
Specification
neurospheres
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk/PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti Rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 50 kDa
Positive control
Control cell
Negative control
gestational age Down syndrome cell
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Tom Hu
Verified customer
投稿 Apr 19 2012