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  1. Link

    pax6-antibody-ab5790.pdf

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Anti-PAX6 抗体 (ab5790)

  • Datasheet
  • SDS
Reviews (22)Q&A (18)References (75)

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Abpromise

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Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab5790)
  • Western blot - Anti-PAX6 antibody (ab5790)
  • Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab5790)
  • Immunoprecipitation - Anti-PAX6 antibody (ab5790)
  • Western blot - Anti-PAX6 antibody (ab5790)
  • Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab5790)

Key features and details

  • Rabbit polyclonal to PAX6
  • Suitable for: IP, ICC/IF, WB
  • Reacts with: Mouse, Rat, Sheep, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-PAX6 antibody [EPR15858] (ab195045)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-PAX6 antibody
    PAX6 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to PAX6
  • 由来種

    Rabbit
  • 特異性

    This antibody does not detect bovine PAX6 by Western blot.

    We have had mixed results for use of this antibody in IHC-Fr. Thus, we are removing IHC-Fr as a guaranteed application and welcome any feedback from customers who have used this antibody in IHC-Fr.

  • アプリケーション

    適用あり: IP, ICC/IF, WBmore details
  • 種交差性

    交差種: Mouse, Rat, Sheep, Human
    交差が予測される動物種: Chicken, Xenopus laevis, Non human primates, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Mouse PAX6 aa 267-285.
    Sequence:

    REEKLRNQRRQASNTPSHI


    Database link: P63015
    (Peptide available as ab5895)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 一次抗体 備考

    PAX genes encode nuclear transcription factors which are regarded as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters’ anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas. All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • PAX
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Developmental Biology
    • Organogenesis
    • Gut development
    • Pancreas development

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunizing Peptide (Blocking)

    • PAX6 peptide (ab5895)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Rat brain cerebrum tissue lysate - total protein (ab4033)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

Our Abpromise guarantee covers the use of ab5790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 5 µg/ml.
ICC/IF 1/50.
WB 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 50.6 kDa).Can be blocked with PAX6 peptide (ab5895).

This antibody can be blocked with ab5895.

ターゲット情報

  • 機能

    Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.
  • 組織特異性

    Fetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form.
  • 関連疾患

    Defects in PAX6 are the cause of aniridia (AN) [MIM:106210]. A congenital, bilateral, panocular disorder characterized by complete absence of the iris or extreme iris hypoplasia. Aniridia is not just an isolated defect in iris development but it is associated with macular and optic nerve hypoplasia, cataract, corneal changes, nystagmus. Visual acuity is generally low but is unrelated to the degree of iris hypoplasia. Glaucoma is a secondary problem causing additional visual loss over time.
    Defects in PAX6 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly consists of a central corneal leukoma, absence of the posterior corneal stroma and Descemet membrane, and a variable degree of iris and lenticular attachments to the central aspect of the posterior cornea.
    Defects in PAX6 are a cause of foveal hypoplasia (FOVHYP) [MIM:136520]. Foveal hypoplasia can be isolated or associated with presenile cataract. Inheritance is autosomal dominant.
    Defects in PAX6 are a cause of keratitis hereditary (KERH) [MIM:148190]. An ocular disorder characterized by corneal opacification, recurrent stromal keratitis and vascularization.
    Defects in PAX6 are a cause of coloboma ocular (COLO) [MIM:120200]; also known as uveoretinal coloboma or coloboma of iris, choroid and retina. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Severe colobomatous malformations may cause as much as 10% of the childhood blindness. The clinical presentation of ocular coloboma is variable. Some individuals may present with minimal defects in the anterior iris leaf without other ocular defects. More complex malformations create a combination of iris, uveoretinal and/or optic nerve defects without or with microphthalmia or even anophthalmia.
    Defects in PAX6 are a cause of coloboma of optic nerve (COLON) [MIM:120430].
    Defects in PAX6 are a cause of bilateral optic nerve hypoplasia (BONH) [MIM:165550]; also known as bilateral optic nerve aplasia. A congenital anomaly in which the optic disc appears abnormally small. It may be an isolated finding or part of a spectrum of anatomic and functional abnormalities that includes partial or complete agenesis of the septum pellucidum, other midline brain defects, cerebral anomalies, pituitary dysfunction, and structural abnormalities of the pituitary.
    Defects in PAX6 are a cause of aniridia cerebellar ataxia and mental deficiency (ACAMD) [MIM:206700]; also known as Gillespie syndrome. A rare condition consisting of partial rudimentary iris, cerebellar impairment of the ability to perform coordinated voluntary movements, and mental retardation.
  • 配列類似性

    Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 paired domain.
  • 発生段階

    Expressed in the developing eye and brain.
  • 翻訳後修飾

    Ubiquitinated by TRIM11, leading to ubiquitination and proteasomal degradation.
  • 細胞内局在

    Nucleus.
  • Target information above from: UniProt accession P26367 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 5080 Human
    • Entrez Gene: 18508 Mouse
    • Entrez Gene: 25509 Rat
    • Entrez Gene: 30567 Zebrafish
    • Omim: 607108 Human
    • SwissProt: P26367 Human
    • SwissProt: P63015 Mouse
    • SwissProt: P63016 Rat
    • SwissProt: P55864 Xenopus laevis
    • SwissProt: P26630 Zebrafish
    • Unigene: 270303 Human
    • Unigene: 611376 Human
    • Unigene: 33870 Mouse
    • Unigene: 3608 Mouse
    • Unigene: 89724 Rat
    • Unigene: 647 Xenopus laevis
    • Unigene: 24244 Zebrafish
    see all
  • 別名

    • AN 2 antibody
    • AN antibody
    • AN2 antibody
    • Aniridia type II protein antibody
    • D11S812E antibody
    • FVH1 antibody
    • KIAA0552 antibody
    • Leucine zipper putative tumor suppressor 3 antibody
    • LZTS3 antibody
    • MGC17209 antibody
    • MGDA antibody
    • Oculorhombin antibody
    • Paired box 6 antibody
    • Paired box gene 6 (aniridia keratitis) antibody
    • Paired Box Gene 6 antibody
    • Paired box homeotic gene 6 antibody
    • Paired box protein Pax-6 antibody
    • Paired box protein Pax6 antibody
    • PAX 6 antibody
    • PAX6 antibody
    • PAX6_HUMAN antibody
    • ProSAP-interacting protein 1 antibody
    • PROSAPIP1 antibody
    • Sey antibody
    • WAGR antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab5790)
    Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab5790)

    Immunofluorescent analysis of PAX6 (green) in All-Trans-Retinoic Acid (ATRA) treated (10 µM for 24 hours) NCCIT cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with a PAX6 polyclonal antibody(ab5790) at a dilution of 1:50 for at least 1 hour at room temperature and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.

  • Western blot - Anti-PAX6 antibody (ab5790)
    Western blot - Anti-PAX6 antibody (ab5790)

    Western blot analysis of PAX6 was performed by loading 50ug of various Human, mouse and non-human primate whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a PAX6 polyclonal antibody (ab5790) at a dilution of 1:1000 overnight at 4°C on a rocking platform then washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:20000 for at least one hour. Chemiluminescent detection was performed.

  • Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab5790)
    Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab5790)Image from Letzen BS et al, PLoS One. 2010 May 5;5(5):e10480, Fig 1.
    ab5790 at a 1/20 dilution staining PAX6 (green) in cryosectioned human embryoid bodies by Immunocytochemistry/ Immunofluorescence.
  • Immunoprecipitation - Anti-PAX6 antibody (ab5790)
    Immunoprecipitation - Anti-PAX6 antibody (ab5790)

    Immunoprecipitation of PAX6 was performed on 293T cells. Antigen: antibody complexes were formed by incubating 500 µg whole cell lysate with 5 µg of PAX6 polyclonal antibody (ab5790) overnight on a rocking platform at 4°C. The immune complexes were captured on 50 µl Protein A/G Agarose, washed extensively and eluted with 5X Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel then transferred to a PVDF membraneand blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a PAX6 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4°C then washed in TBST and probed with detection reagent at a dilution of 1:1000 for at least one hour. Chemiluminescent detection was performed.

  • Western blot - Anti-PAX6 antibody (ab5790)
    Western blot - Anti-PAX6 antibody (ab5790)
    All lanes : Anti-PAX6 antibody (ab5790)

    Lane 1 : rat whole eye extract
    Lane 2 : ovine retinal extract

    Predicted band size: 50.6 kDa
    Observed band size: 47 kDa
    why is the actual band size different from the predicted?



    Shows a Western blot of PAX6 on ovine retinal extract using ab5790.
  • Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab5790)
    Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab5790)Image courtesy of Tom Hu by Abreview.
    ab5790 staining PAX6 in murine brain cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, blocked using 5% BSA for 1 hour at room temperature and then incubated with ab5790 at a 1/250 dilution for 4 hours at 4°C. The secondary used was a Cy2 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.

    See Abreview

プロトコール

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (75)

    ab5790 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab5790 は 75 報の論文で使用されています。

    • Guo W  et al. The circular RNA FAM169A functions as a competitive endogenous RNA and regulates intervertebral disc degeneration by targeting miR-583 and BTRC. Cell Death Dis 11:315 (2020). PubMed: 32366862
    • Bian B  et al. Exosomes derived from neural progenitor cells preserve photoreceptors during retinal degeneration by inactivating microglia. J Extracell Vesicles 9:1748931 (2020). PubMed: 32373289
    • Sekine K  et al. Robust detection of undifferentiated iPSC among differentiated cells. Sci Rep 10:10293 (2020). PubMed: 32581272
    • Grossert A  et al. Ketamine Increases Proliferation of Human iPSC-Derived Neuronal Progenitor Cells via Insulin-Like Growth Factor 2 and Independent of the NMDA Receptor. Cells 8:N/A (2019). PubMed: 31554266
    • Will L  et al. Dynein activating adaptor BICD2 controls radial migration of upper-layer cortical neurons in vivo. Acta Neuropathol Commun 7:162 (2019). PubMed: 31655624
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 40 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PAX6 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Monodelphis domestica Cell (Cortex)
    Permeabilization
    Yes - 0,1% Triton X-100 in PBS
    Specification
    Cortex
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Jelena Ban

    Verified customer

    投稿 May 29 2019

    Immunocytochemistry abreview for Anti-PAX6 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (neuroectoderm)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    neuroectoderm
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 04 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PAX6 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Dog Cell (neurosphere)
    Permeabilization
    Yes - 0.025% TritonX
    Specification
    neurosphere
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 May 04 2018

    Western blot abreview for Anti-PAX6 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (hTCEpi)
    Gel Running Conditions
    Reduced Denaturing (12.5)
    Loading amount
    30 µg
    Specification
    hTCEpi
    Blocking step
    TBS Blocking agent as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Mr. Thanos Papadimitropoulos

    Verified customer

    投稿 Mar 22 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PAX6 antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Xenopus laevis Tissue sections (Whole larvae head)
    Antigen retrieval step
    None
    Permeabilization
    Yes - Triton x-100
    Specification
    Whole larvae head
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 04 2015

    IHC - Wholemount abreview for Anti-PAX6 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Xenopus laevis Tissue (Larval Cornea)
    Specification
    Larval Cornea
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 04 2015

    Western blot abreview for Anti-PAX6 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Xenopus laevis Tissue lysate - whole (Eye)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    5 µg
    Specification
    Eye
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 04 2015

    Western blot abreview for Anti-PAX6 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (10%gel)
    Sample
    Human Cell lysate - whole cell (Human ESCs)
    Specification
    Human ESCs
    Treatment
    Insulin 10ug
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 May 30 2014

    Western blot abreview for Anti-PAX6 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10% Gel)
    Sample
    Mouse Tissue lysate - whole (mouse cerebellum)
    Specification
    mouse cerebellum
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 24 2014

    Question

    Thank you for your email, I’m sending you details of our protocols   For human embryoid bodies:   1 - Cells were fixed in paraformaldehyde, blocked with 5% BSA for 1 hour at room temperature (RT) and incubated with primary antibody at a 1/100 and 1/200 dilutions for O.N. at RT. The secondary used was AlexaFluor 594 Goat anti-Rabbit used at a 1/500 dilution for 1 hour at RT.   2 - Cells were fixed in Bouin’s Solution, blocked with 5% BSA for 1 hour at room temperature (RT) and incubated with primary antibody at a 1/100 and 1/200 dilutions for O.N. at RT. The secondary used was AlexaFluor 594 Goat anti-Rabbit used at a 1/500 dilution for 1 hour at RT.   For mouse neuronal stem cells:   We use the same conditions and also we used another blocker solution but didn’t work   We always saw a cytosolic mark   I’m sending you some pictures from neuronal stem cells, these cells express GFP (green) and were incubate with anti-PAX6 antibody (ab5790) (Red) in blue is DAPI   Thank you for your help  

    Read More

    Abcam community

    Verified customer

    Asked on Dec 07 2012

    Answer

    Thank you for providing these details.

    I am very sorry that this product has not been performing as expected. Having reviewed the protocol details and images I can see no reason which would explain this incorrect staining. I am very sorry for the inconvenience this has caused. I would be very happy to provide a free of charge replacement, whether with a new vial or new anti-Pax6 antibody for you. Alternatively, I can also provide a credit note/refund for this product. In order to proceed could you please provide the Abcam order conformation number for this product or the PO and date of purchase?

    I look forward to hearing how you would like to proceed. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Dec 07 2012

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