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Anti-PAX5 抗体 (ab15164)

Reviews (2)Q&A (2)References (5)
Immunoprecipitation - Anti-PAX5 antibody (ab15164)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody (ab15164)

Key features and details

  • Rabbit polyclonal to PAX5
  • Suitable for: IHC-P, IP
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-PAX5 antibody [EPR3730(2)] (ab109443)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-PAX5 antibody
    PAX5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to PAX5

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, IPmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human PAX5 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q02548

  • 特記事項

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab15164の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/50.
IP
Use a concentration of 5 µg/ml.
特記事項
IHC-P
1/50.
IP
Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能

    May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.

  • 関連疾患

    A chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.
    Leukemia, acute lymphoblastic, 3

  • 配列類似性

    Contains 1 paired domain.

  • 発生段階

    Expressed at early B-cell differentiation, in the developing CNS and in adult testis.

  • 翻訳後修飾

    O-glycosylated.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • B cell lineage specific activator antibody
      • B cell lineage specific activator protein antibody
      • B cell specific activator protein antibody
      • B cell specific transcription factor antibody
      • B-cell-specific transcription factor antibody
      • BSAP antibody
      • EBB-1 antibody
      • KLP antibody
      • Paired box 5 antibody
      • Paired box gene 5 (B cell lineage specific activator protein) antibody
      • Paired box gene 5 (B cell lineage specific activator) antibody
      • Paired box gene 5 antibody
      • Paired box homeotic gene 5 antibody
      • Paired box protein Pax 5 antibody
      • Paired box protein Pax-5 antibody
      • Paired domain gene 5 antibody
      • PAX 5 antibody
      • PAX5 antibody
      • PAX5_HUMAN antibody
      • Transcription factor PAX 5 antibody
      see all

    画像

    • Immunoprecipitation - Anti-PAX5 antibody (ab15164)
      Immunoprecipitation - Anti-PAX5 antibody (ab15164)

      PAX5 was immunoprecipitated using 0.5mg Ramos whole cell extract, 5µg of Rabbit polyclonal to PAX5 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

      The antibody was incubated under agitation with Protein G beads for 10min, Ramos whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab15164.

      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

      Band: 42kDa; PAX5

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody (ab15164)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody (ab15164)
      ab15164 staining PAX5 in human tonsil by Immunohistochemistry (FFPE-sections).

    参考文献 (5)

    ab15164 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab15164 は 5 報の論文で使用されています。

    • Moroki T  et al. Databases for technical aspects of immunohistochemistry: 2021 update. J Toxicol Pathol 34:161-180 (2021). PubMed: 33976473
    • Autry RJ  et al. Integrative genomic analyses reveal mechanisms of glucocorticoid resistance in acute lymphoblastic leukemia. Nat Cancer 1:329-344 (2020). PubMed: 32885175
    • Furukawa S  et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017). IHC ; Dog . PubMed: 28190929
    • Perearnau A  et al. p27Kip1, PCAF and PAX5 cooperate in the transcriptional regulation of specific target genes. Nucleic Acids Res 45:5086-5099 (2017). PubMed: 28158851
    • Katsushima K  et al. Contribution of MicroRNA-1275 to Claudin11 Protein Suppression via a Polycomb-mediated Silencing Mechanism in Human Glioma Stem-like Cells. J Biol Chem 287:27396-406 (2012). WB ; Human . PubMed: 22736761

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-PAX5 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (hematopoietic human cell lines)
    Loading amount
    10 µg
    Specification
    hematopoietic human cell lines
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Mrs. Grazia Fazio

    Verified customer

    投稿 Jul 23 2010

    Western blot abreview for Anti-PAX5 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse pre-B lymphocytes)
    Loading amount
    5 µg
    Specification
    mouse pre-B lymphocytes
    Gel Running Conditions
    Reduced Denaturing (15%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Mrs. Grazia Fazio

    Verified customer

    投稿 Jul 23 2010

    Question

    Pax5 und Mum1
    Kunde sucht polyclonale AK um eine Amplifizierung zu erreichen. Facs

    Read More
    Answer

    Vielen Dank für Ihren Anruf gestern.

    Ich habe für Sie unsere anti Pax5 Antikörper durchgeschaut und muss Ihnen leider mitteilen, dass auch alle polyklonalen mittels Immunogen-Peptiden hergestellt wurden. Diese werden demnach höchstwahrscheinlich auch nur einmal pro Zielprotein binden und keine Amplifikation liefern.

    Meines Wissens ist PE ein sehr "helles" Fluorochrom und sollte zum Sortieren gut geeignet sein, obwohl es schnell ausbleicht.

    Da Sie Zielproteine im Kern färben, könnte die schwache Färbung daran liegen, dass der sekundäre nicht optimal bindet, da einfach nicht genug Platz ist. Vielleicht kann man das durch Antikörper- Fragmente wie F(ab)2 verbessern.

    Bei anti Mum1 Antikörpern haben wir einen, der mit einem Proteinfragment hergestellt worden ist. Aber dass dieser Antikörper an multiplen Stellen bindet und somit das Signal amplifiziert kann ich Ihnen nicht garantieren, da es ja oft ein dominantes Epitop gibt:

    ab78312 (Anti-MUM1 antibody)

    https://www.abcam.com/MUM1-antibody-ab78312.html (or use the following: https://www.abcam.com/MUM1-antibody-ab78312.html).

    Es tut mir leid, dass ich keine positiven Nachrichten für Sie habe und hoffe, diese Information ist dennoch hilfreich.

    Read More

    Question

    The customer would like to know if the antibody will recognise her protein fragment whic is aa306-391.

    Read More
    Answer

    I can confirm that the immunogen sequence is within this amino acid segment and therefore the antibody will recognise your fragment,

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com