Anti-Patched / PTCH1 抗体 (ab109096)
Key features and details
- Goat polyclonal to Patched / PTCH1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Patched / PTCH1 antibody
Patched / PTCH1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Patched / PTCH1 -
由来種
Goat -
特異性
ab109096 is expected to recognise all reported isoforms. Variants (NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1 encode the same isoform. -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Chicken, Xenopus laevis
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免疫原
Synthetic peptide corresponding to Human Patched/ PTCH1 aa 1400 to the C-terminus (C terminal).
Database link: Q13635 -
ポジティブ･コントロール
- Human Colon tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab109096 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109096の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
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| IHC-P | (1) |
Use a concentration of 8 - 0 µg/ml.
Steamed antigen retrieval with citrate buffer pH 6. |
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 10 µg/ml. |
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IHC-P
Use a concentration of 8 - 0 µg/ml. Steamed antigen retrieval with citrate buffer pH 6. |
ターゲット情報
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機能
Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. -
組織特異性
In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin. -
関連疾患
Defects in PTCH1 are probably the cause of basal cell nevus syndrome (BCNS) [MIM:109400]; also known as Gorlin syndrome or Gorlin-Goltz syndrome. BCNS is an autosomal dominant disease characterized by nevoid basal cell carcinomas (NBCCS) and developmental abnormalities such as rib and craniofacial alterations, polydactyly, syndactyly, and spina bifida. In addition, the patients suffer from a multitude of tumors like basal cell carcinomas (BCC), fibromas of the ovaries and heart, cysts of the skin, jaws and mesentery, as well as medulloblastomas and meningiomas. PTCH1 is also mutated in squamous cell carcinoma (SCC). Could also be associated with large body size observed in BCNS patients.
Defects in PTCH1 are a cause of sporadic basal cell carcinoma (BCC) [MIM:605462].
Defects in PTCH1 are the cause of holoprosencephaly type 7 (HPE7) [MIM:610828]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. -
配列類似性
Belongs to the patched family.
Contains 1 SSD (sterol-sensing) domain. -
発生段階
In the embryo, found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud. -
翻訳後修飾
Glycosylation is necessary for SHH binding. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 395806 Chicken
- Entrez Gene: 5727 Human
- Entrez Gene: 89830 Rat
- Omim: 601309 Human
- SwissProt: Q90693 Chicken
- SwissProt: Q13635 Human
- SwissProt: Q86XG7 Human
- Unigene: 494538 Human
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別名
- A230106A15Rik antibody
- BCNS antibody
- FLJ26746 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Patched / PTCH1 antibody (ab109096)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cortex tissue labelling Patched / PTCH1 with ab109096 at 8 µg/mL.
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Immunocytochemistry/ Immunofluorescence - Anti-Patched / PTCH1 antibody (ab109096)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr at 10 ug/mL followed by Alexa Fluor 488 secondary antibody at 2 ug/mL, showing Golgi/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG at 10 ug/mL followed by Alexa Fluor 488 secondary antibody at 2 ug/mL.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab109096 は 3 報の論文で使用されています。
- Pan Y et al. Dysfunction of Shh signaling activates autophagy to inhibit trophoblast motility in recurrent miscarriage. Exp Mol Med 53:52-66 (2021). PubMed: 33390589
- Tang S et al. Regulation of Ptch1 by miR-342-5p and FoxO3 Induced Autophagy Involved in Renal Fibrosis. Front Bioeng Biotechnol 8:583318 (2020). PubMed: 33251198
- Tao H et al. Epigenetic aberrations of miR-369-5p and DNMT3A control Patched1 signal pathway in cardiac fibrosis. Toxicology 410:182-192 (2018). PubMed: 30114436