Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (neurons of human cerebral organoid)
Permeabilization
Yes - Triton X-100
Specification
neurons of human cerebral organoid
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 37°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 4% Donkey serum with 0.1% Triton in PBS
Dilution
1/300
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Goat anti- rabbit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Radhika Menon
Verified customer
投稿 Jul 12 2017