Anti-PARP4 抗体 [P193-10] (ab24110)
Key features and details
- Mouse monoclonal [P193-10] to PARP4
- Suitable for: IHC-P, IHC-FoFr
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-PARP4 antibody [P193-10]
PARP4 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [P193-10] to PARP4 -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, IHC-FoFrmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide, corresponding to amino acids 408-611 of Human PARP4.
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ポジティブ・コントロール
- IHC-P: FFPE human kidney tissue sections.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.3
Preservative: 0.1% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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ポリ/モノ
モノクローナル -
クローン名
P193-10 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24110の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | ||
IHC-FoFr |
特記事項 |
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IHC-F: 1/10 - 1/20.
IHC-Fr: 1/10 - 1/20.
IHC-P: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. preferably with ECL
Predicted molecular weight: 193 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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組織特異性
Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas. -
配列類似性
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 1 VIT domain.
Contains 1 VWFA domain. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Also found in the nucleus, associated with mitotic spindles. - Information by UniProt
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参照データベース
- Entrez Gene: 143 Human
- Omim: 607519 Human
- SwissProt: Q9UKK3 Human
- Unigene: 744855 Human
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別名
- 193 kDa vault protein antibody
- 193kDa vault protein antibody
- ADP ribosyltransferase (NAD+ poly (ADP ribose) polymerase) like 1 antibody
see all
画像
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IHC image of PARP4 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24110, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab24110 は 3 報の論文で使用されています。
- Wu H et al. Single-cell intratumoral stemness analysis reveals the involvement of cell cycle and DNA damage repair in two different types of esophageal cancer. Oncol Rep 41:3201-3208 (2019). PubMed: 31002369
- Szaflarski W et al. Expression profiles of vault components MVP, TEP1 and vPARP and their correlation to other multidrug resistance proteins in ovarian cancer. Int J Oncol 43:513-20 (2013). PubMed: 23739867
- Li B et al. Conditional Akt activation promotes androgen-independent progression of prostate cancer. Carcinogenesis 28:572-83 (2007). PubMed: 17032658