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  1. Link

    parathyroid-hormone-antibody-d11-ab14498.pdf

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Signal Transduction Growth Factors/Hormones Hormones
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Anti-Parathyroid Hormone 抗体 [D1.1] (ab14498)

  • Datasheet
Reviews (1)Q&A (5)

Product price, shipping and contact information

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Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Mouse monoclonal [D1.1] to Parathyroid Hormone
  • Suitable for: IHC-P, ELISA, IHC-Fr
  • Reacts with: Dog, Human
  • Isotype: IgG1

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Parathyroid Hormone antibody [SP151] (ab130759)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Parathyroid Hormone antibody [D1.1]
    Parathyroid Hormone 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [D1.1] to Parathyroid Hormone
  • 由来種

    Mouse
  • アプリケーション

    適用あり: IHC-P, ELISA, IHC-Frmore details
  • 種交差性

    交差種: Dog, Human
    交差が予測される動物種: Cow, Cynomolgus monkey
  • 免疫原

    Synthetic peptide:

    KKEDNVLVESHEKSLGEADKADVNVLTKAKSQ

    , corresponding to amino acids 53-84 of Human Parathyroid Hormone.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    pH: 7.40
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Protein G purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    D1.1
  • アイソタイプ

    IgG1
  • 研究分野

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Parathyroid Hormone protein (ab51234)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab14498の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (1)
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

Use at a concentration of 500 ng/ml.

IHC-Fr
Use at an assay dependent concentration.

ILMA: Use at a concentration of 500 ng/ml.

特記事項
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

Use at a concentration of 500 ng/ml.

IHC-Fr
Use at an assay dependent concentration.

ILMA: Use at a concentration of 500 ng/ml.

ターゲット情報

  • 機能

    PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
  • 関連疾患

    Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
  • 配列類似性

    Belongs to the parathyroid hormone family.
  • 細胞内局在

    Secreted.
  • Target information above from: UniProt accession P01270 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 280903 Cow
    • Entrez Gene: 403986 Dog
    • Entrez Gene: 5741 Human
    • Omim: 168450 Human
    • SwissProt: P01268 Cow
    • SwissProt: P52212 Dog
    • SwissProt: P01270 Human
    • Unigene: 37045 Human
    • 別名

      • hPTH antibody
      • Parathormone antibody
      • Parathyrin antibody
      • Parathyroid hormone 1 antibody
      • Parathyroid hormone antibody
      • Prepro PTH antibody
      • Preproparathyroid hormone antibody
      • PTH antibody
      • PTH1 antibody
      • PTH1 receptor antibody
      • PTH1R antibody
      • PTHR antibody
      • PTHR1 antibody
      • PTHY_HUMAN antibody
      see all

    プロトコール

    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (0)

    ab14498 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab14498 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Parathyroid Hormone antibody [D1.1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (parathyroid)
    Specification
    parathyroid
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Target Retrieval Solution (DAKO) pH 6.0, Microwave 2 x 10 min/700 watt
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 23 2011

    Question

    As we discussed, please see the attached Nanodrop data regarding the 3 replacement vials you just sent.

    Please let me know if you have any questions.

    Thanks!


    Best Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 03 2012

    Answer

    Thank you very much for your call today and for forwarding this data.

    I'll keep you updated of the situation and let you know if I find out any more information. Please see below for information about your credit note and refund.

    Your credit note ID is ***.

    I'm sorry for all of the trouble with thisantibody. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund.

    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require furtherassistance.

    Read More

    Abcam Scientific Support

    Answered on Aug 03 2012

    Question

    Thanks for following up, please see my end user’s response to your QC process.

    The Bradford assay is a total protein assay, a spectrophotometer to measure protein A280/260 is the proper way to determine the IgG content. Clearly their antibody has other protein contaminants if it pass the Bradford assay but shows only 20% of the IgG content on spectrophotometer. We are paying on the basis of IgG content not total protein.

    Best Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 01 2012

    Answer

    Thank you very much for forwarding the comments on to me.

    I'm not sure how the IgG concentration can be specifically measured with a spectrophotometer if there were other proteins in the vial. All proteins (containing aromatic residues) will absorb at 280nm. The only method I'm aware of for measuring IgG concentration in a solution with other proteins (such as serum or tissue culture supernatant) would be by ELISAagainst an IgG standard curve. I've discussed this with my colleagues, and we're wondering if the user has a reference for this method, or could he/she describe the protocol?

    The antibody has been protein G purified and no proteins are added, so we expect it to be quite pure andintend to provide100 ug of IgG per vial. I see that the 3 new vials were delivered today, so if theend user does wish to test these vials then please keep me updated of the results. If the measurements are also coming up short or if the user prefers not to test them in light of the QC information, please let me know and we can arrange a credit or refund.

    I apologize again for all of the inconvenience, and if there is anything else that we can do for you please let me know.

    Read More

    Abcam Scientific Support

    Answered on Aug 01 2012

    Question

    Two vials only contained 20 ug each (should have been 100 ug). How is the mass per vial QC'ed for each new lot?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 31 2012

    Answer

    Thank you again for all of your patience regarding this issue.

    I've heard back from the lab, and each new lot of antibody is tested after production (i.e. before lyophilization) using a Bradford assay and then is tested again after lyophilizaiton.

    Please keep me updated once the new vials are received,and let me know if there is anything else that we can do for you. Have a great day.

    Read More

    Abcam Scientific Support

    Answered on Jul 31 2012

    Question

    Two vials only contained 20 ug each (should have been 100 ug). How is the mass per vial QC'ed for each new lot?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 24 2012

    Answer

    Thanks again for all of your patience regarding this issue. I've set up the replacement order, and these vials will arrive on the order *** sometime next week. I will let you know as soon as I have more information from the lab about the QC process for the new lot.

    Please let me know if there is anything else that we can do for you in the meantime. Have a nice day!

    Read More

    Abcam Scientific Support

    Answered on Jul 24 2012

    Question

    Each vial only contained 0.2 mg/mL after reconsitution with 100 uL as instructed. Should have been 1 mg/mL. Concentration measured by calibrated Nanodrop.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 16 2012

    Answer

    Thank you for your call today and for letting us know about the trouble with ab14498.

    I apologize that I wasn't clear about when I would get back to you. I'll be working with the lab to determine the cause of this problem and fix it, but I'm also sending a vial from a new lot on the order *** which will arrive tomorrow. Please keep me updated about the mass in this vial, and if it is correct then I will send out two more vials from the same lot.

    I look forward to hearing from you. Thank you for your patience, and I am sorry for this inconvenience. If there is anything else that we can do for you please let me know. Have a nice day.

    Read More

    Abcam Scientific Support

    Answered on Jul 16 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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