Key features and details
- Goat polyclonal to PAR6
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab6022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 5 - 10 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
組織特異性Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.
配列類似性Belongs to the PAR6 family.
Contains 1 OPR domain.
Contains 1 PDZ (DHR) domain.
Contains 1 pseudo-CRIB domain.
ドメインThe pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.
The OPR domain mediates interactions with MAP2K5.
The PDZ domain mediates the interaction with CRB3.
細胞内局在Cytoplasm. Cell membrane. Cell projection > ruffle. Cell junction > tight junction. Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions.
- Information by UniProt
- 0710008C04Rik antibody
- 2610010A15Rik antibody
- Par 6 partitioning defective 6 C elegans homolog alpha antibody
Ab6022 (10ug/ml) staining human PAR6 in human pancreas by immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Ab6022 shows strong cytoplasmic staining in rare cells of intralobular ducts.
ab6022 at 5ug/ml staining PAR6 in human heart tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
ab6022 は 1 報の論文で使用されています。
- Wu M et al. Epicardial Spindle Orientation Controls Cell Entry into the Myocardium. Dev Cell 19:114-125 (2010). IHC-P ; Mouse . PubMed: 20643355