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  1. Link

    par6-antibody-ab6022.pdf

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Epigenetics and Nuclear Signaling Cell cycle Cell Differentiation
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Anti-PAR6 抗体 (ab6022)

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Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR6 antibody (ab6022)

    Key features and details

    • Goat polyclonal to PAR6
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    こちらの製品もご検討ください

    タンパク質
    Product image
    Recombinant Human PAR6 protein (ab132012)
    二次抗体
    Product image
    Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    ノックアウト
    Product image
    Human PARD6A (PAR6) knockout HeLa cell line (ab265171)

    関連製品

    製品の概要

    • 製品名

      Anti-PAR6 antibody
      PAR6 一次抗体 製品一覧
    • 製品の詳細

      Goat polyclonal to PAR6
    • 由来種

      Goat
    • アプリケーション

      適用あり: IHC-Pmore details
    • 種交差性

      交差種: Human
    • 免疫原

      Synthetic peptide corresponding to Human PAR6 aa 300 to the C-terminus (C terminal). (NP_001032358.1)
      Database link: NP_058644.1

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • バッファー

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • 特記事項(精製)

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Epigenetics and Nuclear Signaling
      • Cell cycle
      • Cell Differentiation
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cell Adhesion
      • Tight Junctions
      • Cell Biology
      • Cell Cycle
      • Cell Division
      • Other cell division antibodies
      • Cancer
      • Cell cycle
      • Cell division
      • Stem Cells
      • Cancer Stem Cells
      • Intracellular

    関連製品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Recombinant Protein

      • Recombinant Human PAR6 protein (ab132012)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab6022の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    Use a concentration of 5 - 10 µg/ml.
    特記事項
    IHC-P
    Use a concentration of 5 - 10 µg/ml.

    ターゲット情報

    • 機能

      Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
    • 組織特異性

      Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.
    • 配列類似性

      Belongs to the PAR6 family.
      Contains 1 OPR domain.
      Contains 1 PDZ (DHR) domain.
      Contains 1 pseudo-CRIB domain.
    • ドメイン

      The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.
      The OPR domain mediates interactions with MAP2K5.
      The PDZ domain mediates the interaction with CRB3.
    • 細胞内局在

      Cytoplasm. Cell membrane. Cell projection > ruffle. Cell junction > tight junction. Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions.
    • Target information above from: UniProt accession Q9NPB6 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 50855 Human
      • Omim: 607484 Human
      • SwissProt: Q9NPB6 Human
      • Unigene: 112933 Human
      • 別名

        • 0710008C04Rik antibody
        • 2610010A15Rik antibody
        • Par 6 partitioning defective 6 C elegans homolog alpha antibody
        • Par 6 partitioning defective 6 homolog alpha antibody
        • Par 6 partitioning defective 6 homolog alpha C elegans antibody
        • PAR 6A antibody
        • PAR-6 alpha antibody
        • PAR-6 antibody
        • par-6 family cell polarity regulator alpha antibody
        • PAR-6A antibody
        • PAR6 antibody
        • PAR6A_HUMAN antibody
        • PAR6alpha antibody
        • PAR6C antibody
        • Pard6a antibody
        • Partitioning defective 6 homolog alpha antibody
        • partitioning-defective protein 6 antibody
        • partitioning-defective protein 6, C. elegans, homolog of, alpha antibody
        • Tax interacting protein 40 antibody
        • Tax interaction protein 40 antibody
        • TAX40 antibody
        • TIP 40 antibody
        • TIP-40 antibody
        • TIP40 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR6 antibody (ab6022)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR6 antibody (ab6022)
        Ab6022 (10ug/ml) staining human PAR6 in human pancreas by immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

        Ab6022 shows strong cytoplasmic staining in rare cells of intralobular ducts.

      プロトコール

      • Immunohistochemistry protocols

      Click here to view the general protocols

      データシートおよび資料

      • Datasheet download

        Download

      参考文献 (2)

      ab6022 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab6022 は 2 報の論文で使用されています。

      • Tapia R  et al. Enteropathogenic Escherichia coli (EPEC) Recruitment of PAR Polarity Protein Atypical PKC? to Pedestals and Cell-Cell Contacts Precedes Disruption of Tight Junctions in Intestinal Epithelial Cells. Int J Mol Sci 21:N/A (2020). PubMed: 31947656
      • Wu M  et al. Epicardial Spindle Orientation Controls Cell Entry into the Myocardium. Dev Cell 19:114-125 (2010). IHC-P ; Mouse . PubMed: 20643355

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-3 of 3 Abreviews or Q&A

      Question

      Dear,
      I'm a PhD student working at EMBL (European Molecular Biology Laboratory).
      I'm looking for an antibody anti-PAR6 (Partion Defective 6). I found in
      lecterature that abcam was selling an antibody able to recognize all the 3
      isoform of the protein catalog number ab45394. Unfortunately this product
      is not anymore available. It is something temporally or will be not sell
      anymore? I look the other antibody available but in the datasheet is not
      specificated if they react with all the 3 isoform of the protein. There is
      a specific product in substitution of ab45394?
      thank you in advance.
      Kinds Regards

      Read More

      Abcam community

      Verified customer

      Asked on Jun 06 2012

      Answer

      Thank you for your inquiry.

      Unfortunately, we only have one antibody in our catalog at the moment that is tested to work with mouse. The immunogen of does not show a high homology with mouse but the antibody was used successfully in the following publication:

      PubMed: 20643355, Figure 7 IHC.

      The antibody most likely binds to the last coupleof amino acids in the immunogen. This would also mean that all three isoform should be recognized.

      But this has not been experimentally tested and we do not know yetthat this antibody is good for mouse under all circumstances and applications in all mouse samples.

      I am sorry I did not have positive and straight forward information for you on this occasion and hope this information is nevertheless helpful to you.

      Read More

      Abcam Scientific Support

      Answered on Jun 06 2012

      Question

      Status of: #****** : received in late february #**** : Unfortunately ab45394 is now unpublished. studied species : Mouse application : IF, WB, IP

      Read More

      Abcam community

      Verified customer

      Asked on Nov 24 2011

      Answer

      Comme convenu, la commande numéro ****** (une unité gratuite de ab6022) a été expédiée aujourd'hui. Vous devriez recevoir le produit demain. J'espère que l'anti-PAR6 ab6022 fonctionnera chez la souris. Nous serions ravis si vous pouviez nous envoyer une Abreview avec votre feedback sur l'utilisation de ab6022 chez la souris. Le lien pour soumettre cette Abreview est : https://www.abcam.com/PAR6-antibody-ab6022-reviews.html N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

      Read More

      Abcam Scientific Support

      Answered on Nov 24 2011

      Question

      We have injected the homogenized tumor tissue isolated from human lung into the armpit of mice. After certain time the neoplasm is formed in the armpit. Now we want to know the nature of the neoplasm that is originally from human lung tumor or is a new tumor from mouse. We would like to check the nature of the neoplasm by IHC (florescence). Could you please tell us which antibody that can specifically recognize human tissue (tumor) or mouse tissue (tumor) ? We hope that antibody does not cross-react on human or mouse. Also, the host of antibody is not form mouse.

      Read More

      Abcam community

      Verified customer

      Asked on May 09 2007

      Answer

      Thank you for contacting us. I have heard of people using anti-vimentin clone V9, to detect human tissue implanted in mice. However, this is a mouse monoclonal and our version, ab8069 is unconjugated. We do not have a version conjugated to a fluorochrome, enzyme, or other detector. An anti-mouse IgG secondary may give some staining of endogenous IgG, as you are probably aware. For detecting mouse tissue without detecting human, consider ab15630, with is also available biotinylated as ab15631 and FITC-conjugated as ab15638: https://www.abcam.com/MHC-Class-II-antibody-ER-TR3-ab15630.html The ab15630 and ab15631 have been shown to work on frozen tissue, but will not work on paraffin-embedded tissue. Ab15638 has only been tested for flow cytometry so we canot guarantee it will work for IHC. Antibody ab6022 has not been tested on mouse tissue. An alignment of the immunogenic peptide with the mouse protein shows that it is 69% identical; it is difficult to predict from this if the antibody will or will not cross-react with the mouse protein. The alignment can be found at this address for the next 24 hours: http://www.ebi.ac.uk/cgi-bin/clustalw/result?tool=clustalw&jobid=clustalw-20070510-00025988&poll=yes I hope this helps; please do not hesitate to contact us if you have any questions.

      Read More

      Abcam Scientific Support

      Answered on May 10 2007

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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