PAP pen (ab2601)

Reviews (4)Q&A (5)References (4)
PAP pen (ab2601)

    製品の概要

    • 製品名

      PAP pen

    • 特記事項

      The PAP pen is a special marking pen that provides a thin film-like green-tinged hydrophobic barrier when a circle is drawn around a specimen on a slide.

      This water repellent barrier keeps staining reagents localized on the tissue sections, prevents mixing of reagents when differentially staining two sections on the same slide, and allows use of less reagents per section.

      Insoluble in alcohol and acetone, PAP Pen markings can be optionally removed with xylene after the staining procedure is complete.

      PAP Pen is good for approximately 500 uses.

      The formulation of PAP pen has been optimised to increase water repellence and tolerance against alcohol and surface active agent, and is now a lighter colour.

    製品の特性

    関連製品

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      • PAP pen (ab2601)
        PAP pen (ab2601)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (4)

      ab2601 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab2601 は 4 報の論文で使用されています。

      • Ledowski T  et al. Effects of muscle relaxants on ischaemia damage in skeletal muscle. Sci Rep 8:5794 (2018). PubMed: 29643396
      • Duffy MF  et al. Lewy body-like alpha-synuclein inclusions trigger reactive microgliosis prior to nigral degeneration. J Neuroinflammation 15:129 (2018). PubMed: 29716614
      • Deng Y  et al. Improved i.p. drug delivery with bioadhesive nanoparticles. Proc Natl Acad Sci U S A 113:11453-11458 (2016). PubMed: 27663731
      • Aydin K  et al. Effect of epidermal growth factor receptor status on the outcomes of patients with metastatic gastric cancer: A pilot study. Oncol Lett 7:255-259 (2014). PubMed: 24348859

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      1-9 of 9 Abreviews or Q&A

      super pen

       Excellent Excellent 5/5 (Ease of Use)
      Abreviews
      The pen works well. It`s easy to use and dries quickly. The barrier is precise and reliable - even after 24h incubation. It is resistant to buffer but can be removed with xylene.The pen is a great help in the IHC when there are multiple tissue samples on the same slide. And you save a lot of reagent, because you can frame the samples as small as possible.

      Abcam user community

      Verified customer

      投稿 Mar 06 2018

      high quality PAP pen

       Excellent Excellent 5/5 (Ease of Use)
      Abreviews
      abreview image
      This PAP pen is very easy to use. It dries quickly. It is strong enough to create and maintain a boundary between tissue sections. I usually stain secondary antibody control on the same slide with primary antibody, so I divided the section with the PAP pen. After overnight incubation with antibody, the boundary still functional and there was no mixing between adjacent drop of solutions. Picture was taken after overnight incubation.

      Abcam user community

      Verified customer

      投稿 Dec 02 2016

      PAP pen

       Good Good 4/5 (Ease of Use)
      Abreviews
      abreview image
      PAP pen dries quickly and forms a hydrophobic layer that is not washed off by 0.1% Triton X-100 in PBS. The only concern I have is that the barrier is often difficult to dry after washing, so extra care must be taken to avoid mixing reagents between adjacent sections.

      Mr. Bryan Niedenberger

      Verified customer

      投稿 Aug 31 2016

      Question

      What is the thickness of the pen.

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      Answer

      The tip of the Pap pen is about 3 mm thick and tapers off to about 1 mm.

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      Barrier pen

       Excellent Excellent 5/5 (Ease of Use)
      Abreviews
      Precise and reliable barrier pen. After application it dries rather quick and after that there's no leakage. Found it very useful for IHC when I had to isolate from each other few tissue samples on the same slide. It resists incubation in buffers with Tween-20. Soluble in Xylene.

      Mr. Vasily Gnyubkin

      Verified customer

      投稿 May 02 2014

      Question

      Can the PAP pen be used at temperatures around 200°C?

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      Answer

      The PAP pen can be used at temperatures as high as 48°C (120°F). When working with temperatures higher than 48°C, the protective barrier may be compromised and loses its water-repellant properties.

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      Question

      I have a customer who is interested in AB2601 PAP PEN, it is indicated that each unit is good for approximately 500 uses. The customer wants to know what is the volume in the per unit ? I am looking forward to your reply. .

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      Answer

      Thank you for your enquiry. We have not calculated the volume of liquid used per unit, however I can confirm that one unit is equivalent to drawing with the pen along the four sides of a standard IHC slide, such that a hydrophobic barrier is created to ensure the tissue remains moist upon application of antibody. I hope this information will help your customer.

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      Question

      Is the PAP pen incompatible with solutions containing 50% formamide? I used a PAP pen to draw a barrier around tissue sections on glass slides, then overlaid the sections with a hybridization solution containing 50% formamide and 50% standard saline citrate (aqueous NaCl and sodium citrate). After an overnight incubation at 42 degrees in a humid chamber, some of the PAP barriers appeared to have partially disappeared--dissolved? The hybridization solution had flowed over, and there were floating green flecks (like bits of the barrier). I redrew the barrier before doing additional incubations in aqueous solutions, but the redrawn barriers seemed to have trouble adhering to the slides. I've used PAP pens before with aqueous solutions, with good results. So I wonder if the problem is the formamide.

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      Answer

      Thank you for your enquiry. The PAP pen has been used for IHC only. You should wipe the section in a "square- coverslip" fashion if you wish to use it for ISH. (At least the size of the cover slip.) The line that is drawn must be very dry before placing a small amount of probe on the tissue. I am unsure whether formamide is a xylene solvent, however we do not believe that the PAP pen will withstand formamide;if you use the pen on the outside of the cover slip that may work. Alternatively you could try using barrier slides? Good luck with your research.

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      Question

      Can the tissue section be encircled with the PAP-pen when the surface is wet?

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      Answer

      No, we recommend wiping the tissue dry before applying the pen to ensure the barrier does not spread across the tissue.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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