Name: Anti-PAP antibody [EPR4066]
Brand: RabMAb
SKU: ab108984
Rating: 4 (1 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4066] to PAP
Suitable for: WB, IP, IHC-P
Reacts with: Human

Carrier Free

製品名

Anti-PAP antibody [EPR4066]
PAP 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR4066] to PAP
由来種

Rabbit
アプリケーション

適用あり: WB, IP, IHC-Pmore details
適用なし: Flow Cyt
種交差性

交差種: Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Prostate cancer cell lysate. IHC: human prostatic and human prostatic adenocarcinoma tissues. IP: human prostate lysate.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
解離定数（K_D 値）

K_D = 1.41 x 10 ^-10 M
Learn more about K_D
バッファー

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR4066
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant human PAP protein (Active) (ab219224)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab108984の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB		1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IP		1/10 - 1/100.
IHC-P	(1)	1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended.

特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended.

追加情報

Is unsuitable for Flow Cyt.

配列類似性

Belongs to the histidine acid phosphatase family.
細胞内局在

Secreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles.
Target information above from: UniProt accession P15309 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 55 Human
- Omim: 171790 Human
- SwissProt: P15309 Human
- Unigene: 433060 Human
別名
- 5' NT antibody
- 5' nucleotidase antibody
- 5'-NT antibody
- 5'-nucleotidase antibody
- Acid phosphatase prostate antibody
- ACP 3 antibody
- ACP3 antibody
- acpP antibody
- Ecto 5' nucleotidase antibody
- Ecto-5'-nucleotidase antibody
- PAP antibody
- PPAP_HUMAN antibody
- Prostatic acid phosphatase antibody
- Prostatic acid phosphotase antibody
- Thiamine monophosphatase antibody
- TMPase antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

Immunohistochemical staining of paraffin-embedded human prostatic tissue using anti-PAP (ab108984).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-PAP antibody [EPR4066] (ab108984)

Anti-PAP antibody [EPR4066] (ab108984) at 1/1000 dilution + Prostate cancer cell lysate at 10 µg

Predicted band size: 45 kDa
Immunoprecipitation - Anti-PAP antibody [EPR4066] (ab108984)

PAP was immunoprecipitated from 0.35 mg Human prostate whole cell lysate 10 µg with 108984 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Human prostate whole cell lysate 10 µg

Lane 2: ab108984 IP in Human prostate whole cell lysate

Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab108984 in Human prostate whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

Immunohistochemical staining of paraffin-embedded human prostatic adenocarcinoma tissue using anti-PAP (ab108984).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

ab108984 showing negative staining in Skeletal muscle tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

ab108984 showing negative staining in Colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

ab108984 showing negative staining in Normal tonsil tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

ab108984 showing negative staining in Normal placenta tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

ab108984 showing positive staining in Prostatic carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

ab108984 showing negative staining in Breast carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

ab108984 showing negative staining in Cervical carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] (ab108984)

ab108984 showing negative staining in Normal breast tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
OI-RD Scanning - Anti-PAP antibody [EPR4066] (ab108984)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-PAP antibody [EPR4066] (ab108984)

Immunoprecipitation protocols
Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab108984 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108984 は 3 報の論文で使用されています。

Liu T et al. FSIP1 binds HER2 directly to regulate breast cancer growth and invasiveness. Proc Natl Acad Sci U S A 114:7683-7688 (2017). PubMed: 28674022
Wei J et al. Rapid progression of mixed neuroendocrine carcinoma-acinar adenocarcinoma of the prostate: A case report. Oncol Lett 12:1019-1022 (2016). IHC-P ; Human . PubMed: 27446387
Ge R et al. DNA methyl transferase 1 reduces expression of SRD5A2 in the aging adult prostate. Am J Pathol 185:870-82 (2015). PubMed: 25700986

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Prostate Cancer)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: EDTA based buffer pH 9.0

Permeabilization

Specification

Prostate Cancer

Blocking step

Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 23°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

投稿 Nov 13 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

解離定数（KD 値）

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (3)

レビューと Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Prostatic Acid Phosphatase antibody [EPR4066]

解離定数（K_D 値）