Anti-pan Keratin 抗体 [80] (ab8068)

Reviews (8)Q&A (10)References (23)

Key features and details

  • Mouse monoclonal [80] to pan Keratin
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-pan Keratin antibody [80]
    pan Keratin 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [80] to pan Keratin

  • 由来種

    Mouse

  • アプリケーション

    適用あり: Flow Cyt, WB, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Callus cytokeratins isolated from fresh human skin tissue

  • ポジティブ･コントロール

    • Human callus or keratinocytes

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.3
    Preservative: 0.07% Sodium azide
    Constituent: 1% Fetal calf serum
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    80

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/20.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
WB Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 関連性

    Cytokeratin is a family of basic and acidic proteins, present in dermal tissues. Each cytokeratin is formed by heterotetramers of different types of keratin, that span from 1 to 18. In keratinized epidermis, 50 kD keratin is present in the basal layer, while 56.5 kD keratin is present in suprabasal layers, where as 58 kD keratin is present in the basal and suprabasal layers, while 65 to 67 kD keratin is present in the cells above the basal layers.

  • 細胞内局在

    Cytoplasmic

  • 参照データベース

    • 別名

      • CK1 antibody
      • Cytokeratin 1 antibody
      • EHK1 antibody
      • K1 antibody
      • Keratin 1 antibody
      • KRT1 antibody
      • KRT1A antibody
      • KRTA antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)
      Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)
      ICC/IF image of ab8068 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)
      Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)This image is courtesy of an anonymous Abreview

      Ab8068 staining pan Keratin in Human glioblastoma cell line by ICC/IF (Immunocytochemistry/Immunofluorescence). The cells were fixed with paraformaldehyde; permeabilized with 0.1% Triton X 100 in PBS and blocked with 0.5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50 dilution in 0.5% BSA in PBS) for 16hours at 4°C. An Alexa Fluor® 488 anti-mouse was used as a secondary antibody at 1/400 dilution.

      See Abreview

    • Western blot - Anti-pan Keratin antibody [80] (ab8068)
      Western blot - Anti-pan Keratin antibody [80] (ab8068)
      Anti-pan Keratin antibody [80] (ab8068) at 1/250 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 65 kDa
      Observed band size: 65 kDa
      Additional bands at: 49 kDa, 58 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds
    • Flow Cytometry - Anti-pan Keratin antibody [80] (ab8068)
      Flow Cytometry - Anti-pan Keratin antibody [80] (ab8068)
      Overlay histogram showing A431 cells stained with ab8068 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8068, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)
      Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)Image courtesy of an anonymous Abreview.
      ab8068 staining pan Keratin in rat glioblastoma cell line C6 by Immunocytochemistry/ Immunofluorescence.
      Cells were fixed in paraformaldehyde, permeabilized using 0,1% Triton X 100 in PBS, blocked with 0.5% BSA for 30 minutes at room temperature and then incubated with ab8068 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a Cy3 conjugated goat anti-mouse polyclonal used at a 1/400 dilution. Nuclei are counterstained with DAPI.

      See Abreview

    参考文献 (23)

    ab8068 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab8068 は 23 報の論文で使用されています。

    • Hu J  et al. Effects of menstrual blood-derived stem cells on endometrial injury repair. Mol Med Rep 19:813-820 (2019). PubMed: 30569163
    • Chartoumpekis DV  et al. Nrf2 prevents Notch-induced insulin resistance and tumorigenesis in mice. JCI Insight 3:N/A (2018). PubMed: 29515034
    • Nie X  et al. mTOR acts as a pivotal signaling hub for neural crest cells during craniofacial development. PLoS Genet 14:e1007491 (2018). PubMed: 29975682
    • Madeo M  et al. Cancer exosomes induce tumor innervation. Nat Commun 9:4284 (2018). PubMed: 30327461
    • Zheng J  et al. Epithelial Cdc42 Deletion Induced Enamel Organ Defects and Cystogenesis. J Dent Res 97:1346-1354 (2018). PubMed: 29874522
    View all Publications for this product

    レビューと Q&A

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    1-10 of 18 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Keratin antibody [80]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Hair follicle)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH=6
    Permeabilization
    No
    Specification
    Hair follicle
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 07 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Keratin antibody [80]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Keratinocytes (HaCaT))
    Permeabilization
    No
    Specification
    Keratinocytes (HaCaT)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 07 2020

    Immunocytochemistry abreview for Anti-pan Keratin antibody [80]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cell (Keratinocytes (HaCaT))
    Permeabilization
    No
    Specification
    Keratinocytes (HaCaT)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: PBS°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 07 2020

    Question

    I was wondering whether the abcam 8068 pan Keratin antibody might also work for rabbit tissue. More specifically we would like to use this antibody to stain the cornea which is mainly positive for Keratin 3 and 12 (also for CK 14 and 15). Would this Ab be able to detect (Cyto)Keratin 3 and 12?

    Read More
    Answer

    We are not aware of any tests of rabbit samples with the pan-keratin antibody ab8068. The production and initial characterization of the antibody are described in the two 1984 papers at these links.


    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1900394/

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1900469/


    I think the designation of the antibody as "pan-keratin" is based on the following comment from the Discussion section of the Jan 1984 paper, but we have not seen data from tests of purified keratins 3, 12, or others, or data from studies of cornea, so we can't say for sure that the antibody will be capable of detecting those keratins.


    It is clear from our staining results on normal tissues and malignant epithelial tumors that Clone 80 is a very broadly reacting antibody directed against an antigenic determinant common to a number of cytokeratins present in almost all epithelial cells.

    Read More

    Immunohistochemistry (Frozen sections) abreview for Anti-pan Keratin antibody [80]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin (psoriasis))
    Permeabilization
    No
    Specification
    Skin (psoriasis)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Acetone
    Read More

    Herr Dr. Christian Ostalecki

    Verified customer

    投稿 May 24 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Keratin antibody [80]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human glioblastoma cell line)
    Specification
    human glioblastoma cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0,1% Triton X 100 in PBS
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 26 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Keratin antibody [80]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (glioblastoma cell line C6)
    Specification
    glioblastoma cell line C6
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0,1% Triton X 100 in PBS
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 26 2012

    Question

    I am looking to get some prepared control slides for the Anti-pan Keratin antibody [80] (ab8068) on your website. The overview of your antibody product suggests that the positive control is Human Callus or keratinocytes. I am looking to purchase prepared slides of the suggested control and was curious if you offered such a product or could point me in the right direction? Additionally, how much would these slides possibly cost?   Thank you for your time and assistance.    

    Read More
    Answer

    Thank you for contacting Abcam. I have looked through our catalogue to see if we have any prepared slides of human callus or keratinocytes. I have not been able to find anything that matches your requirements, but I have placed a link below that will show all of the prepared slides that we do have: www.abcam.com/index.html?pageconfig=catalog_byclass&intClassID=4186&strStartChar=A,B I also did a quick search of other companies to see if they had anything that matches your requirements but I could not find anything. Please let me know if there is anything else I can help with.  

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    Question

    I came across primary antibody ab51054. Has this antibody ever been tested for its cross-reactivity against pig??? Thank you so much for all your help. Sincerely,

    Read More
    Answer

    Thank you for your question. We do not have any information about cross reactivity with pig. Please do not hesitate to contact us in case of any additional questions or remarks.

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    Question

    Thank you so much for the quick reply. I have another question. For my particular experiment I am co-culturing porcine periodontal ligament epithelial cells with mouse fibroblast cells. I need a primary antibody to distinguish these two different cells from one another. (mouse fibroblast cells have green fluorescent protein so double immunostaining is not necessary). Could you please recommend me with a primary antibody (preferably host animal to be a rabbit please) for this co-culture experiment. (my main goal is to distinguish two cells from each other). Thank you so much for all your help. Sincerely,

    Read More
    Answer

    Thank you for getting back to me and for explaining a little bit more about your experiments. It may be worth applying antibodies which recognize pig albumin or serum (ab79960, ab50337, ab79957). Unfortunately, currently we do not have many antibodies specific for porcine proteins. The majority of our antibodies has been routinely tested in human, mouse and rat. You could use antibodies against mouse MHC (class I) to detect and distinguish mouse cells from porcine cells. We have different clonality antibodies against mouse MHC class I, please have a look and use our new search engine. You can also filter through the search results by different criteria (such as application, reactivity, clonality): https://www.abcam.com/ I hope this helps and if I can assist further, please do not hesitate to contact me.

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    1-10 of 18 Abreviews or Q&A

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