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  1. Link

    pan-keratin-antibody-80-ab8068.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules Cytokeratin
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Anti-pan Keratin 抗体 [80] (ab8068)

  • Datasheet
  • SDS
Reviews (12)Q&A (10)References (32)

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Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)
  • Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)
  • Western blot - Anti-pan Keratin antibody [80] (ab8068)
  • Flow Cytometry - Anti-pan Keratin antibody [80] (ab8068)

Key features and details

  • Mouse monoclonal [80] to pan Keratin
  • Suitable for: Flow Cyt, IHC-Fr, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

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関連製品

製品の概要

  • 製品名

    Anti-pan Keratin antibody [80]
    pan Keratin 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [80] to pan Keratin
  • 由来種

    Mouse
  • アプリケーション

    適用あり: Flow Cyt, IHC-Fr, IHC-P, WB, ICC/IFmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Full length native protein (purified) corresponding to Human pan Keratin. Callus cytokeratins isolated from fresh human skin tissue

  • ポジティブ・コントロール

    • Human callus or keratinocytes
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.3
    Preservative: 0.07% Sodium azide
    Constituent: 1% Fetal calf serum
  • Concentration information loading...
  • 精製度

    Tissue culture supernatant
  • ポリ/モノ

    モノクローナル
  • クローン名

    80
  • アイソタイプ

    IgG1
  • 研究分野

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Cytokeratin

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab8068の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
1/20.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-Fr (2)
Use at an assay dependent concentration.

Stains all types of keratin containing cells (epithelia) in frozen sections of various tissues, with the exception of myoepithelial cells.

IHC-P (3)
Use at an assay dependent concentration.

For paraffin embedded tissue a TUF pretreatment is recommended.

WB (1)
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
ICC/IF (5)
Use a concentration of 1 µg/ml.
特記事項
Flow Cyt
1/20.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-Fr
Use at an assay dependent concentration.

Stains all types of keratin containing cells (epithelia) in frozen sections of various tissues, with the exception of myoepithelial cells.

IHC-P
Use at an assay dependent concentration.

For paraffin embedded tissue a TUF pretreatment is recommended.

WB
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
ICC/IF
Use a concentration of 1 µg/ml.

ターゲット情報

  • 関連性

    Cytokeratin is a family of basic and acidic proteins, present in dermal tissues. Each cytokeratin is formed by heterotetramers of different types of keratin, that span from 1 to 18. In keratinized epidermis, 50 kD keratin is present in the basal layer, while 56.5 kD keratin is present in suprabasal layers, where as 58 kD keratin is present in the basal and suprabasal layers, while 65 to 67 kD keratin is present in the cells above the basal layers.
  • 細胞内局在

    Cytoplasmic
  • 参照データベース

    • Entrez Gene: 3848 Human
    • Omim: 139350 Human
    • SwissProt: P04264 Human
    • SwissProt: P04104 Mouse
    • 別名

      • CK1 antibody
      • Cytokeratin 1 antibody
      • EHK1 antibody
      • K1 antibody
      • Keratin 1 antibody
      • KRT1 antibody
      • KRT1A antibody
      • KRTA antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)
      Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)
      ICC/IF image of ab8068 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)
      Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)This image is courtesy of an anonymous Abreview

      Ab8068 staining pan Keratin in Human glioblastoma cell line by ICC/IF (Immunocytochemistry/Immunofluorescence). The cells were fixed with paraformaldehyde; permeabilized with 0.1% Triton X 100 in PBS and blocked with 0.5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50 dilution in 0.5% BSA in PBS) for 16hours at 4°C. An Alexa Fluor® 488 anti-mouse was used as a secondary antibody at 1/400 dilution.

      See Abreview

    • Western blot - Anti-pan Keratin antibody [80] (ab8068)
      Western blot - Anti-pan Keratin antibody [80] (ab8068)
      Anti-pan Keratin antibody [80] (ab8068) at 1/250 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 65 kDa
      Observed band size: 65 kDa
      Additional bands at: 49 kDa, 58 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds
    • Flow Cytometry - Anti-pan Keratin antibody [80] (ab8068)
      Flow Cytometry - Anti-pan Keratin antibody [80] (ab8068)
      Overlay histogram showing A431 cells stained with ab8068 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8068, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    プロトコール

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (32)

    ab8068 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab8068 は 32 報の論文で使用されています。

    • Shan G  et al. Downregulated exosomal microRNA-148b-3p in cancer associated fibroblasts enhance chemosensitivity of bladder cancer cells by downregulating the Wnt/ß-catenin pathway and upregulating PTEN. Cell Oncol (Dordr) 44:45-59 (2021). PubMed: 33423167
    • Lu C  et al. Tumor necrosis factor receptor type 1-associated death domain (TRADD) regulates epithelial-mesenchymal transition (EMT), M1/M2 macrophage polarization and ectopic endometrial cysts formation in endometriosis. Ann Transl Med 9:148 (2021). PubMed: 33569450
    • Li J  et al. WJ-MSCs intervention may relieve intrauterine adhesions in female rats via TGF-β1-mediated Rho/ROCK signaling inhibition. Mol Med Rep 23:N/A (2021). PubMed: 33179074
    • Franco-Barraza J  et al. Engineering clinically-relevant human fibroblastic cell-derived extracellular matrices. Methods Cell Biol 156:109-160 (2020). PubMed: 32222216
    • Cao J  et al. Estrogen attenuates TGF-ß1-induced EMT in intrauterine adhesion by activating Wnt/ß-catenin signaling pathway. Braz J Med Biol Res 53:e9794 (2020). PubMed: 32638833
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 22 Abreviews or Q&A

    Western blot abreview for Anti-pan Keratin antibody [80]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (A549 (pulmonary epithelial cells))
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    A549 (pulmonary epithelial cells)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 17 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Keratin antibody [80]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Keratinocytes)
    Permeabilization
    No
    Specification
    Keratinocytes
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 15 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Keratin antibody [80]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Epithelial cells)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Specification
    Epithelial cells
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 22 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-pan Keratin antibody [80]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Cervix)
    Permeabilization
    No
    Specification
    Cervix
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 08 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Keratin antibody [80]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Hair follicle)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH=6
    Permeabilization
    No
    Specification
    Hair follicle
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 07 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Keratin antibody [80]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Keratinocytes (HaCaT))
    Permeabilization
    No
    Specification
    Keratinocytes (HaCaT)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 07 2020

    Immunocytochemistry abreview for Anti-pan Keratin antibody [80]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cell (Keratinocytes (HaCaT))
    Permeabilization
    No
    Specification
    Keratinocytes (HaCaT)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: PBS°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 07 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-pan Keratin antibody [80]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin (psoriasis))
    Permeabilization
    No
    Specification
    Skin (psoriasis)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Acetone
    Read More

    HERR DR. Christian Ostalecki

    Verified customer

    投稿 May 24 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Keratin antibody [80]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human glioblastoma cell line)
    Specification
    human glioblastoma cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0,1% Triton X 100 in PBS
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 26 2012

    Question

    I was wondering whether the abcam 8068 pan Keratin antibody might also work for rabbit tissue. More specifically we would like to use this antibody to stain the cornea which is mainly positive for Keratin 3 and 12 (also for CK 14 and 15). Would this Ab be able to detect (Cyto)Keratin 3 and 12?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 06 2018

    Answer

    We are not aware of any tests of rabbit samples with the pan-keratin antibody ab8068. The production and initial characterization of the antibody are described in the two 1984 papers at these links.


    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1900394/

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1900469/


    I think the designation of the antibody as "pan-keratin" is based on the following comment from the Discussion section of the Jan 1984 paper, but we have not seen data from tests of purified keratins 3, 12, or others, or data from studies of cornea, so we can't say for sure that the antibody will be capable of detecting those keratins.


    It is clear from our staining results on normal tissues and malignant epithelial tumors that Clone 80 is a very broadly reacting antibody directed against an antigenic determinant common to a number of cytokeratins present in almost all epithelial cells.

    Read More

    Abcam Scientific Support

    Answered on Aug 06 2018

    1-10 of 22 Abreviews or Q&A

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