Anti-pan Cytokeratin 抗体 [PCK-26] (ab6401)

Reviews (19)Q&A (8)References (35)
Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)
  • Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)

Key features and details

  • Mouse monoclonal [PCK-26] to pan Cytokeratin
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG1

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Cytokeratin 18 antibody [EPR17347] (ab181597)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-pan Cytokeratin antibody [PCK-26]
    pan Cytokeratin 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [PCK-26] to pan Cytokeratin

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Rat, Rabbit, Goat, Chicken, Cow, a wide range of other species

  • 免疫原

    Full length native protein (purified) corresponding to Human pan Cytokeratin.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • 特記事項（精製）

    Purified from TCS.

  • ポリ/モノ

    モノクローナル

  • クローン名

    PCK-26

  • ミエローマ

    unknown

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    unknown

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6401の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (5)
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.

  • 細胞内局在

    Cytoplasmic

  • 参照データベース

    • 別名

      • pan ck antibody
      • pan-ck antibody
      • panck antibody

    画像

    • Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)
      Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)
      Lane 1 : Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 2.5 µg/ml
      Lane 2 : Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 5 µg/ml
      Lane 3 : Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 10 µg/ml

      All lanes : HeLa cell lysate

      Secondary
      All lanes : Peroxidase-conjugated goat anti-mouse IgG


      This image was generated using the ascites version of the product.

    • Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)
      Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)This image is courtesy of an Abreview submitted by Pawel Mazur

      All lanes: pan Cytokeratin antibody (ab6401) at 1/1000 dilution + whole cell lysate of Primary mouse pancreatic cancer cell lines (35 µg)

      Secondary: An HRP-conjugated Sheep anti-mouse IgG polyclonal (1/5000) developed using the ECL technique

      Performed under non-reducing conditions.

      Blocking Step: 10% Milk for 1 hour at room temperature

      This image was generated using the ascites version of the product.

      See Abreview

    参考文献 (35)

    ab6401 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6401 は 35 報の論文で使用されています。

    • Casey KM  et al. Proliferative Typhlocolitis With Multinucleated Giant Cells: A Nonspecific Enteropathy in Immunodeficient Sentinel Mice. Vet Pathol 56:157-168 (2019). PubMed: 30222063
    • Erickson-DiRenzo E  et al. Methodology for the establishment of primary porcine vocal fold epithelial cell cultures. Laryngoscope 129:E355-E364 (2019). PubMed: 30848488
    • Li Y  et al. Human iPSC-Derived Natural Killer Cells Engineered with Chimeric Antigen Receptors Enhance Anti-tumor Activity. Cell Stem Cell 23:181-192.e5 (2018). PubMed: 30082067
    • Meisel M  et al. Microbial signals drive pre-leukaemic myeloproliferation in a Tet2-deficient host. Nature 557:580-584 (2018). PubMed: 29769727
    • Hong HN  et al. Cancer-associated fibroblasts promote gastric tumorigenesis through EphA2 activation in a ligand-independent manner. J Cancer Res Clin Oncol 144:1649-1663 (2018). PubMed: 29948146
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    1-10 of 27 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [PCK-26]

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Synovial Tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6 & TRIS pH 9
    Specification
    Synovial Tissue
    Blocking step
    10% Donkey serum + 3% BSA as blocking agent as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative
    Formaldehyde
    Read More

    Dr. Dylan Windell

    Verified customer

    投稿 Apr 23 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [PCK-26]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Mouse Primary Tumor (Mammary Gland))
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate Buffer
    Permeabilization
    No
    Specification
    Mouse Primary Tumor (Mammary Gland)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 26°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Francesco Elia Marino

    Verified customer

    投稿 Sep 25 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [PCK-26]

     Good
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Normal kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    No
    Specification
    Normal kidney
    Blocking step
    (agent) for 15 minute(s) · Concentration: 3% · Temperature: 27°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 24 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [PCK-26]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6.0
    Sample
    Pig Tissue sections (Skin)
    Specification
    Skin
    Permeabilization
    Yes - 0.05% Tween 20
    Fixative
    10% Formalin
    Read More

    Mr. Jesse Wu

    Verified customer

    投稿 Oct 30 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [PCK-26]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6.0
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Permeabilization
    Yes - 0.05% Tween 20
    Fixative
    10% Formalin
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 29 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [PCK-26]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6.0
    Sample
    Rabbit Tissue sections (Skin)
    Specification
    Skin
    Permeabilization
    Yes - 0.05% Tween 20
    Fixative
    10% Formalin
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 29 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [PCK-26]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Mouse lung)
    Specification
    Mouse lung
    Fixative
    Formaldehyde
    Antigen retrieval step
    None
    Permeabilization
    Yes - Trion X-100
    Blocking step
    2% horse serum plus mouse Fab IgG fragment as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 28 2013

    Question

    I need specification (data) sheets, with specific protein concentrations per lot#, for various products that I used by Abcam. This information must be include in my file to be archived at work. ab16667 - Lot# GR59808-2 ab6401 - Lot# GR1281-5 I found generic data sheets online but I need the documented protein concentration for the actual lot numbers I used. Somebody misplaced the original paperwork that came with the products.

    Read More
    Answer

    Thank you for contacting us.
    Attached are the two Certificates of Compliance you have requested.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    To Whom It May Concern:

    I would like to ask you for anti-pan Cytokeratin antibody product: What is the different between anti-pan cytokeratin antibody(PCK-26) code # ab6401 and anti-pan cytokeratin antibody (PCK-26) code # ab11214. ?. I also would like to know more information for your product, anti-pan Cytokeratin antibody (AE1+AE3) prediluted code # ab961. Thank you.

    Read More
    Answer

    Thank you for contacting us.

    The antibodies ab6401 and ab11214 are from the same clone [PCK-26], so they are the same antibody with similar binding characteristics. The difference is that ab6401 is not purified and is not conjugated while ab11214 is purified and conjugated to FITC.

    The mouse monoclonal [AE1+AE3] to pan Cytokeratin, prediluted, ab961 is already diluted and ready to use for cell and tissue staining right out of the vial. You receive 6 ml of this pre-diluted antibody.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Hi Technical,

    Our customer has bought 2 antibodies ab6401 and ab1416. He wants to know whether these two antibodies can be expressed by Epidermal Keratinocytes (as they are epithelial cells as well) or no?


    The other question is what is the antibody that we can use which is not expressed by epithelial cells (Epidermal Keratinocytes)?

    We look forward to your soonest reply.

    Thank you and best regards

    Read More
    Answer

    Thank you for contacting us.

    I have found some references which support that the pan Cytokeratin and E Cadherin would be expressed by epidermal keratinocytes:

    http://jmm.sgmjournals.org/content/56/7/971.full

    http://www.univet.es/_bibliography/development_characterization_of_a_canine_skin.pdf

    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC275417/

    http://jcb.rupress.org/content/117/2/415

    If your customer is interested in using an antibody to differentiate between these cells and other epithelial cells I would suggest that they do a review of the literature to see which markers have been used in their field of research for these purposes. If they have a particular target they are interested in please do let me know and I will see if I can suggest a suitable antibody.

    I hope this information has been of help. If you require any further information, please do not hesitate to contact us again.

    Read More

    1-10 of 27 Abreviews or Q&A

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