Anti-pan Cytokeratin 抗体 [PCK-26] (ab6401)
![Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)](https://www.abcam.co.jp/ps/products/6/ab6401/Images/ab6401-275027-anti-pan-cytokeratin-antibody-pck-26-western-blot.jpg "Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)")
Key features and details
- Mouse monoclonal [PCK-26] to pan Cytokeratin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-pan Cytokeratin antibody [PCK-26]
pan Cytokeratin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [PCK-26] to pan Cytokeratin -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Rabbit, Goat, Chicken, Cow, a wide range of other species
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免疫原
Full length native protein (purified) corresponding to Human pan Cytokeratin.
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特記事項
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Tissue culture supernatant -
特記事項（精製）
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
PCK-26 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6401の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (5) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 374454 Human
- Entrez Gene: 51350 Human
- SwissProt: Q01546 Human
- SwissProt: Q7Z794 Human
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別名
- pan ck antibody
- pan-ck antibody
- panck antibody
画像
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Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)Lane 1 : Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 2.5 µg/ml
Lane 2 : Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 5 µg/ml
Lane 3 : Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 10 µg/ml
All lanes : HeLa cell lysate
Secondary
All lanes : Peroxidase-conjugated goat anti-mouse IgGThis image was generated using the ascites version of the product.
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![Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)](https://www.abcam.co.jp/ps/products/6/ab6401/Images/ab6401-12295-anti-pan-cytokeratin-antibody-pck-26-western-blot.jpg)
Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)This image is courtesy of an Abreview submitted by Pawel MazurAll lanes: pan Cytokeratin antibody (ab6401) at 1/1000 dilution + whole cell lysate of Primary mouse pancreatic cancer cell lines (35 µg)
Secondary: An HRP-conjugated Sheep anti-mouse IgG polyclonal (1/5000) developed using the ECL technique
Performed under non-reducing conditions.
Blocking Step: 10% Milk for 1 hour at room temperatureThis image was generated using the ascites version of the product.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (35)
ab6401 は 35 報の論文で使用されています。
- Casey KM et al. Proliferative Typhlocolitis With Multinucleated Giant Cells: A Nonspecific Enteropathy in Immunodeficient Sentinel Mice. Vet Pathol 56:157-168 (2019). PubMed: 30222063
- Erickson-DiRenzo E et al. Methodology for the establishment of primary porcine vocal fold epithelial cell cultures. Laryngoscope 129:E355-E364 (2019). PubMed: 30848488
- Li Y et al. Human iPSC-Derived Natural Killer Cells Engineered with Chimeric Antigen Receptors Enhance Anti-tumor Activity. Cell Stem Cell 23:181-192.e5 (2018). PubMed: 30082067
- Meisel M et al. Microbial signals drive pre-leukaemic myeloproliferation in a Tet2-deficient host. Nature 557:580-584 (2018). PubMed: 29769727
- Hong HN et al. Cancer-associated fibroblasts promote gastric tumorigenesis through EphA2 activation in a ligand-independent manner. J Cancer Res Clin Oncol 144:1649-1663 (2018). PubMed: 29948146