Anti-pan Cytokeratin 抗体 [PCK-26] (ab6401)
Key features and details
- Mouse monoclonal [PCK-26] to pan Cytokeratin
- Suitable for: WB
- Reacts with: Mouse, Sheep, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-pan Cytokeratin antibody [PCK-26]
pan Cytokeratin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [PCK-26] to pan Cytokeratin -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Sheep, Human
交差が予測される動物種: Rat, Rabbit, Goat, Chicken, Cow, a wide range of other species
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免疫原
Full length native protein (purified) corresponding to Human pan Cytokeratin.
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特記事項
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Tissue culture supernatant -
特記事項（精製）
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
PCK-26 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab6401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. |
ターゲット情報
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関連性
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 374454 Human
- Entrez Gene: 51350 Human
- SwissProt: Q01546 Human
- SwissProt: Q7Z794 Human
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別名
- pan ck antibody
- pan-ck antibody
- panck antibody
画像
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![Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)](https://www.abcam.co.jp/ps/products/6/ab6401/Images/ab6401-275027-anti-pan-cytokeratin-antibody-pck-26-western-blot.jpg)
Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)Lane 1 : Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 2.5 µg/ml
Lane 2 : Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 5 µg/ml
Lane 3 : Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 10 µg/ml
All lanes : HeLa cell lysate
Secondary
All lanes : Peroxidase-conjugated goat anti-mouse IgGThis image was generated using the ascites version of the product.
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![Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)](https://www.abcam.co.jp/ps/products/6/ab6401/Images/ab6401-12295-anti-pan-cytokeratin-antibody-pck-26-western-blot.jpg)
Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)This image is courtesy of an Abreview submitted by Pawel MazurAll lanes: pan Cytokeratin antibody (ab6401) at 1/1000 dilution + whole cell lysate of Primary mouse pancreatic cancer cell lines (35 µg)
Secondary: An HRP-conjugated Sheep anti-mouse IgG polyclonal (1/5000) developed using the ECL technique
Performed under non-reducing conditions.
Blocking Step: 10% Milk for 1 hour at room temperatureThis image was generated using the ascites version of the product.
プロトコール
参考文献 (32)
ab6401 は 32 報の論文で使用されています。
- Hong HN et al. Cancer-associated fibroblasts promote gastric tumorigenesis through EphA2 activation in a ligand-independent manner. J Cancer Res Clin Oncol 144:1649-1663 (2018). PubMed: 29948146
- Natesan S et al. Peroxisome proliferator-activated receptor-a agonist and all-trans retinoic acid induce epithelial differentiation of subcutaneous adipose-derived stem cells from debrided burn skin. J Cell Biochem N/A:N/A (2018). PubMed: 30556162
- Meisel M et al. Microbial signals drive pre-leukaemic myeloproliferation in a Tet2-deficient host. Nature 557:580-584 (2018). PubMed: 29769727
- He Z et al. Epithelial-derived IL-33 promotes intestinal tumorigenesis in Apc (Min/+) mice. Sci Rep 7:5520 (2017). Flow Cyt . PubMed: 28710436
- Wei M et al. Malignant ascites-derived exosomes promote proliferation and induce carcinoma-associated fibroblasts transition in peritoneal mesothelial cells. Oncotarget 8:42262-42271 (2017). PubMed: 28178689
