Anti-pan Cadherin 抗体 [EPR1792Y] - Intercellular Junction Marker (ab51034)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1792Y] to pan Cadherin - Intercellular Junction Marker
- Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker
pan Cadherin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1792Y] to pan Cadherin - Intercellular Junction Marker -
由来種
Rabbit -
特異性
The immunogen used for this product shares 100% homology with N-cadherin, 100% homology with R-cadherin, 93% homology to K-cadherin, 93% homology to P-cadherin, 93% homology to E-cadherin, and 73% homology to VE-cadherin. Cross-reactivities with these proteins have not been confirmed experimentally.
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アプリケーション
適用あり: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human pan Cadherin (C terminal). The exact sequence is proprietary.
Database link: P19022 -
ポジティブ・コントロール
- WB: C6 whole cell lysate. Mouse heart tissue lysate. Human, mouse, and rat brain tissue lysates; ICC: MCF7 cells; IHC-P: Rat, mouse and human cardiac muscle tissue; human and rat colon and mouse testis tissue; IP: Rat heart lysate; Flow Cyt (intra): HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
解離定数(KD 値)
KD = 6.10 x 10 -10 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1792Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 488 Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab195202)
- Alexa Fluor® 647 Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab195203)
- Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)
- Alexa Fluor® 568 Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab312753)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51034の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/20.
For unpurified use at 1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 100 kDa).
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IP |
1/20.
For unpurified use at 1/40. |
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IHC-P | (2) |
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100. |
ICC/IF | (2) |
1/100 - 1/250.
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特記事項 |
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Flow Cyt (Intra)
1/20. For unpurified use at 1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 100 kDa). |
IP
1/20. For unpurified use at 1/40. |
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100. |
ICC/IF
1/100 - 1/250. |
ターゲット情報
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関連性
Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis. -
参照データベース
- Entrez Gene: 1001 Human
- Entrez Gene: 1002 Human
- Entrez Gene: 1003 Human
- Entrez Gene: 1004 Human
- Entrez Gene: 1005 Human
- Entrez Gene: 1006 Human
- Entrez Gene: 1008 Human
- Entrez Gene: 1009 Human
see all -
別名
- Cadherin antibody
- CDH3 antibody
- CDHP antibody
see all
画像
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All lanes : Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034) at 1/50000 dilution (purified)
Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 2 : Human brain lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 100 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cardiac muscle tissue sections labeling pan Cadherin with purified ab51034 at 1/500 dilution (0.26 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunocytochemistry/Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cells labeling pan Cadherin with purified ab51034 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 was used as counterstain antibody.
Confocal image showing membranous staining in MCF7 cells.
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Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling pan Cadherin with purified ab51034 at 1/20 dilution (10µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Purified ab51034 at 1/20 dilution (0.6 µg) immunoprecipitating pan Cadherin in Rat heart lysate.
Lane 1 (input): Rat heart lysate 10 µg
Lane 2 (+): ab51034 + Rat heart lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51034 in Rat heart lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 140 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue sections labeling pan Cadherin with purified ab51034 at 1/500 dilution (0.26 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling pan Cadherin with purified ab51034 at 1/500 dilution (0.26 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemical analysis of Paraffin-embedded human colon tissue sections labeling pan Cadherin with unpurified ab51034 at 1/100. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).
Positive staining on human colon.
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Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034) at 1/50000 dilution (purified) + Mouse heart lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of Paraffin-embedded rat colon tissue sections labeling pan Cadherin with unpurified ab51034 at 1/100. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).
Positive staining on rat colon.
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Immunohistochemical analysis of Paraffin-embedded mouse testis tissue sections labeling pan Cadherin with unpurified ab51034 at 1/100. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).
Positive staining on mouse testis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab51034 は 16 報の論文で使用されています。
- Fu Q et al. Adverse Maternal Environment and Postweaning Western Diet Alter Hepatic CD36 Expression and Methylation Concurrently with Nonalcoholic Fatty Liver Disease in Mouse Offspring. J Nutr 151:3102-3112 (2021). PubMed: 34486661
- Bai S et al. Exosomal circ_DLGAP4 promotes diabetic kidney disease progression by sponging miR-143 and targeting ERBB3/NF-?B/MMP-2 axis. Cell Death Dis 11:1008 (2020). PubMed: 33230102
- Chen W et al. Blocking interleukin-6 trans-signaling protects against renal fibrosis by suppressing STAT3 activation. Theranostics 9:3980-3991 (2019). PubMed: 31281526
- Zhang C et al. Sulforaphene induces apoptosis and inhibits the invasion of esophageal cancer cells through MSK2/CREB/Bcl-2 and cadherin pathway in vivo and in vitro. Cancer Cell Int 19:342 (2019). PubMed: 31889894
- Chung TW et al. Antitumor effect of kurarinone and underlying mechanism in small cell lung carcinoma cells. Onco Targets Ther 12:6119-6131 (2019). PubMed: 31496721