Key features and details
- Rabbit polyclonal to pan Cadherin
- Suitable for: ICC/IF
- Reacts with: Mouse, Chicken, Cow, Dog, Human, Xenopus laevis, Zebrafish
- Isotype: IgG
製品名Anti-pan Cadherin antibody
pan Cadherin 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to pan Cadherin
アプリケーション適用あり: ICC/IFmore details
種交差性交差種: Mouse, Chicken, Cow, Dog, Human, Xenopus laevis, Zebrafish
交差が予測される動物種: Rabbit, Bird, Fish, Amphibian
This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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一次抗体 備考This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.
Our Abpromise guarantee covers the use of ab6529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100. Fix cells with 2-4% paraformaldehyde for 10 minutes at room temperature, followed by 10-minute incubation with 0.2% Triton X-100 (see Gellersen et al 2007 and Kaur et al 2006).|
関連性Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis.
- Cadherin antibody
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Immunofluorescent imaging of human cells (U2OS) with ab6529 confirms the specificity of this antibody. Pan cadherin antibody recognises predominantly membranous signal with some fainter cytoplasmic staining corresponding to soluble cadherin. No nuclear staining is seen.
IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees C. Nuclei were stained with Hoechst stain.
ab6529 staining pan Cadherin in human bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed and blocked in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted, 1/200 and incubated with sample for 9 hour at 4°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, diluted 1/200 was used as secondary.
ab6529 staining pan Cadherin in murine liver cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.4% Triton X-100 and then blocked using 10% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 17 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 594 (red) used at a 1/1000 dilution. The other secondary antibody used was an Alexa Fluor® 488 (green) goat anti-mouse IgG for detection of a specific cytosolic protein.
ab6529 は 32 報の論文で使用されています。
- Bai Y et al. Structural basis for pharmacological modulation of the TRPC6 channel. Elife 9:N/A (2020). PubMed: 32149605
- Choi EK et al. Ferroportin disease mutations influence manganese accumulation and cytotoxicity. FASEB J 33:2228-2240 (2019). PubMed: 30247984
- Ip JPK et al. Major Vault Protein, a Candidate Gene in 16p11.2 Microdeletion Syndrome, Is Required for the Homeostatic Regulation of Visual Cortical Plasticity. J Neurosci 38:3890-3900 (2018). PubMed: 29540554
- Liu W et al. Ne-fatty acylation of multiple membrane-associated proteins by Shigella IcsB effector to modulate host function. Nat Microbiol 3:996-1009 (2018). PubMed: 30061757
- Choi EK et al. Functional analysis of SLC39A8 mutations and their implications for manganese deficiency and mitochondrial disorders. Sci Rep 8:3163 (2018). PubMed: 29453449