Anti-pan Cadherin 抗体 (ab16505)
Key features and details
- Rabbit polyclonal to pan Cadherin
- Suitable for: ICC, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-pan Cadherin antibody
pan Cadherin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to pan Cadherin -
由来種
Rabbit -
アプリケーション
適用あり: ICC, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Cow, Xenopus laevis, Zebrafish
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免疫原
Synthetic peptide corresponding to Human pan Cadherin aa 850 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab17098) -
ポジティブ･コントロール
- ICC/IF: U2OS; HeLa cells. WB: Mouse Heart; Mouse Muscle; Human Heart; Rat Heart. IHC-P: Human Liver.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16505の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use a concentration of 2 µg/ml.
A diffuse signal is seen throughout the cells if higher concentrations are used (5-10µg/ml). We have had reports that the antibody works less well in this application in murine (3T3) cells. |
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| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 135 kDa (predicted molecular weight: 100 kDa).
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| IHC-P | (2) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
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ICC
Use a concentration of 2 µg/ml. A diffuse signal is seen throughout the cells if higher concentrations are used (5-10µg/ml). We have had reports that the antibody works less well in this application in murine (3T3) cells. |
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 135 kDa (predicted molecular weight: 100 kDa). |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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関連性
Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis. -
参照データベース
- Entrez Gene: 1001 Human
- Entrez Gene: 1002 Human
- Entrez Gene: 1003 Human
- Entrez Gene: 1004 Human
- Entrez Gene: 1005 Human
- Entrez Gene: 1006 Human
- Entrez Gene: 1008 Human
- Entrez Gene: 1009 Human
see all -
別名
- Cadherin antibody
- CDH3 antibody
- CDHP antibody
see all
画像
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Immunocytochemistry - Anti-pan Cadherin antibody (ab16505)
ab16505 staining pan Cadherin in U2OS cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab16505 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Western blot - Anti-pan Cadherin antibody (ab16505)All lanes : Anti-pan Cadherin antibody (ab16505) at 1 µg/ml
Lane 1 : Human heart lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 100 kDa
Observed band size: 125-140 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 65 kDa, 75 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody (ab16505)IHC image of pan Cadherin staining in human liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16505, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Western blot - Anti-pan Cadherin antibody (ab16505)All lanes : Anti-pan Cadherin antibody (ab16505) at 1 µg/ml
Lane 1 : Mouse heart
Lane 2 : HeLa cell lysate
Lane 3 : 3T3 cell lysate
Lane 4 : Mouse muscle
Lane 5 : Human heart
Lane 6 : Mouse heart with Human pan Cadherin peptide (ab17098) at 1 µg/ml
Lane 7 : HeLa cell lysate with Human pan Cadherin peptide (ab17098) at 1 µg/ml
Lane 8 : 3T3 cell lysate with Human pan Cadherin peptide (ab17098) at 1 µg/ml
Lane 9 : Mouse muscle with Human pan Cadherin peptide (ab17098) at 1 µg/ml
Lane 10 : Human heart with Human pan Cadherin peptide (ab17098) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit conjugated to Alexafluor 680 at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 100 kDa -
Immunocytochemistry - Anti-pan Cadherin antibody (ab16505)This image is courtesy of Rosmaria Mangiacasale & Patrizia Lavia, University La SapienzaHeLa cells fixed in methanol and stained with ab16505 (2µg/ml). The cells were fixed in 100% methanol for 6 minutes at -20°C, then washed once in PBS. The 2 images show the cells stained with different secondary antibodies, Donkey anti Rabbit FITC (image A) and Donkey anti Rabbit Cy3 (image B). In each case ab16505 stains the plasma membrane. In image A ab16505 is stained green and in image B ab16505 is stained red. In both images the DNA is stained with DAPI (blue).
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Immunocytochemistry - Anti-pan Cadherin antibody (ab16505)Image from Kiss K et al., PLoS One. 2012;7(5):e37378. Epub 2012 May 24. Fig 1.; doi:10.1371/journal.pone.0037378; May 24, 2012, PLoS ONE 7(5): e37378.Immunofluorescence analysis of HeLa cells, staining pan Cadherin (red) with ab16505.
Cells were fixed with paraformaldehyde, permeabilized in methanol and blocked for 1 hour at room temperature in DPBS containing 2 mg/mL BSA, 1% fish gelatin, 0.1% Triton-X 100 and 5% goat serum. Cells were then incubated for 1 hour at room temperature with the primary antibody diluted in blocking buffer. An AlexaFluor®-conjugated anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (58)
ab16505 は 58 報の論文で使用されています。
- Zheng K et al. CircRNA PIP5K1A promotes the progression of glioma through upregulation of the TCF12/PI3K/AKT pathway by sponging miR-515-5p. Cancer Cell Int 21:27 (2021). PubMed: 33413401
- Deng Y et al. Estradiol/GPER affects the integrity of mammary duct-like structures in vitro. Sci Rep 10:1386 (2020). PubMed: 31992771
- Diofano F et al. Genetic compensation prevents myopathy and heart failure in an in vivo model of Bag3 deficiency. PLoS Genet 16:e1009088 (2020). PubMed: 33137814
- Han L et al. Lamin B2 Levels Regulate Polyploidization of Cardiomyocyte Nuclei and Myocardial Regeneration. Dev Cell 53:42-59.e11 (2020). PubMed: 32109383
- Bu X et al. 1% Isoflurane and 1.2 µg/ml of Propofol: A Combination of Anesthetics That Causes the Least Damage to Hypoxic Neurons. Front Aging Neurosci 12:591938 (2020). PubMed: 33304268