Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (cultured hippocampal astrocytes)
Specification
cultured hippocampal astrocytes
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1%
Other product details
Dilution
1/100
Incubation time
2 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
primary hippocampal astrocytes were maintained for 2 days in culture in the presence of 10 % serum, fixed in 4% paraformaldehyde for 15 min at room temperature, and permeabilized with 0.25% Triton in PBS for 5 min. Primary antibodies and secondary antibodies were applied at room temperature in PBS containing 1%BSA.
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投稿 Mar 08 2007