Application
Western blot
Sample
Human Cell lysate - whole cell (Breast)
Loading amount
20 µg
Specification
Breast
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Other product details
Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 488
Dilution
1/5000
Detection
Detection method
fluorescent
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 65 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 May 18 2009