Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PAK1 (phospho S144) antibody [EP656Y]

Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (normal tissue array (including breast/colon/spleen)
Specification
normal tissue array (including breast/colon/spleen
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10%

Other product details

Dilution
1/400
Incubation time
1 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Mouse anti rabbit HRP
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
All procedures done at room temperature apart from antigen retrieval.

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Verified customer

投稿 May 19 2009

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