PAK1 overexpression 293T lysate (whole cell) (ab94241)
製品の概要
-
製品名
PAK1 overexpression 293T lysate (whole cell)
PAK1 ライゼート 製品一覧 -
特記事項
ab94241 is a 293T cell transfected lysate in which Human PAK1 has been transiently over-expressed using a pCMV-PAK1 Full length plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
-
アプリケーション
適用あり: WBmore details
製品の特性
-
Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
バッファー
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
研究分野
-
背景
Function: The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CRIB domain. Contains 1 protein kinase domain. PTM: Autophosphorylated when activated by CDC42/p21 and RAC1.
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94241の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent dilution.
|
特記事項 |
---|
WB
Use at an assay dependent dilution. |
画像
-
ab94241 at 15μg/lane on an SDS-PAGE gel.
-
All lanes : Anti-PAK1 antibody (ab58263) at 1/500 dilution
Lane 1 :PAK1 overexpression 293T lysate (whole cell) (ab94241)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab94241 は論文での使用が確認できていません。