Anti-PAK1 + PAK2 + PAK3 (phospho T423) 抗体 (ab2477)
Key features and details
- Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho T423)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody
PAK1 + PAK2 + PAK3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho T423) -
由来種
Rabbit -
特異性
This phospho specific polyclonal antibody is specific for phosphorylated Threonine 423 of Human PAK 1 + 2 + 3. Reactivity with non-phosphorylated PAK 1 + 2 + 3 is less than 1% by ELISA. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human PAK1 + PAK2 + PAK3 (phospho T423) conjugated to keyhole limpet haemocyanin. Corresponding to amino acids 413-433 of Human PAK 1, 408-428 of human PAK 2 and 411-431 of human PAK 3.
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特記事項
P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of threonine 423 (threonine 402 for PAK 2 and threonine 421 for PAK 3), catalyzed by Cdc42, is required for activation of PAK.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. -
一次抗体 備考
P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of threonine 423 (threonine 402 for PAK 2 and threonine 421 for PAK 3), catalyzed by Cdc42, is required for activation of PAK. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2477の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/2000. Detects a band of approximately 65 kDa.
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| 特記事項 |
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WB
1/500 - 1/2000. Detects a band of approximately 65 kDa. |
ターゲット情報
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関連性
In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death. -
細胞内局在
Cytoplasm. Recruited to focal adhesions upon activation -
参照データベース
- Entrez Gene: 5058 Human
- Entrez Gene: 5062 Human
- Entrez Gene: 5063 Human
- Omim: 300142 Human
- Omim: 602590 Human
- Omim: 605022 Human
- SwissProt: O75914 Human
- SwissProt: Q13153 Human
see all -
別名
- Alpha PAK antibody
- Beta PAK antibody
- bPAK antibody
see all
画像
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Western blot - Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody (ab2477)
Western blot probing with PAK1 antibody (ab2477). The major band is at 65 kDa and weaker bands can be detected at 90 and 100 kDa. This picture was kindly submitted by Sue Thomas as part of her review.
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Dot Blot - Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody (ab2477)Dot Blot analysis labeling phosphorylated and non-phosphorylated forms of the immunizing peptide with ab2477 at 1/1000 dilution, incubated overnight at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1/40000 dilution for 45 min at room temperature. Block: Blocking Buffer for Fluorescent Western Blotting for 60 min at RT.
Load: 200 ng, 66.7 ng, 22.2 ng, 7.4 ng, or 2.5 ng.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab2477 は 7 報の論文で使用されています。
- Albrecht LV et al. GSK3 Inhibits Macropinocytosis and Lysosomal Activity through the Wnt Destruction Complex Machinery. Cell Rep 32:107973 (2020). PubMed: 32726636
- Baharani A et al. A Progressive Loss of phosphoSer138-Profilin Aligns with Symptomatic Course in the R6/2 Mouse Model of Huntington's Disease: Possible Sex-Dependent Signaling. Cell Mol Neurobiol N/A:N/A (2020). PubMed: 33108594
- Singh NK et al. p115 RhoGEF activates the Rac1 GTPase signaling cascade in MCP1 chemokine-induced vascular smooth muscle cell migration and proliferation. J Biol Chem 292:14080-14091 (2017). PubMed: 28655771
- Kumar R et al. A new role for cofilin in retinal neovascularization. J Cell Sci 129:1234-49 (2016). PubMed: 26857814
- Singh NK et al. Disruption of p21-activated kinase 1 gene diminishes atherosclerosis in apolipoprotein E-deficient mice. Nat Commun 6:7450 (2015). PubMed: 26104863
- Liu JS et al. ß-elemene enhances the radiosensitivity of gastric cancer cells by inhibiting Pak1 activation. World J Gastroenterol 21:9945-56 (2015). PubMed: 26379399
- Gadepalli R et al. Novel Role for p21-activated Kinase 2 in Thrombin-induced Monocyte Migration. J Biol Chem 288:30815-31 (2013). WB . PubMed: 24025335