Anti-PAF-R 抗体 (ab104162)
Key features and details
- Rabbit polyclonal to PAF-R
- Suitable for: ICC, IP, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-PAF-R antibody -
製品の詳細
Rabbit polyclonal to PAF-R -
由来種
Rabbit -
アプリケーション
適用あり: ICC, IP, WB, Flow Cytmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Pig, Rhesus monkey
-
免疫原
Synthetic peptide corresponding to Human PAF-R aa 1-17 (N terminal) conjugated to keyhole limpet haemocyanin.
Sequence:MEPHDSSHMDSEFRYTL
(Peptide available asab107706) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.05% BSA, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104162の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| WB |
1/200. Predicted molecular weight: 39 kDa.Can be blocked with PAF-R peptide (ab107706).
|
|
| Flow Cyt |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
ICC
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
WB
1/200. Predicted molecular weight: 39 kDa.Can be blocked with PAF-R peptide (ab107706). |
|
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
-
機能
Receptor for platelet activating factor, a chemotactic phospholipid mediator that possesses potent inflammatory, smooth-muscle contractile and hypotensive activity. Seems to mediate its action via a G protein that activates a phosphatidylinositol-calcium second messenger system. -
組織特異性
Expressed in the placenta, lung, left and right heart ventricles, heart atrium, leukocytes and differentiated HL-60 granulocytes. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 5724 Human
- Entrez Gene: 19204 Mouse
- Entrez Gene: 58949 Rat
- Omim: 173393 Human
- SwissProt: P25105 Human
- SwissProt: Q62035 Mouse
- SwissProt: Q9XSD4 Pig
- SwissProt: P46002 Rat
see all -
別名
- OTTHUMP00000004041 antibody
- OTTMUSP00000010350 antibody
- PAF R antibody
see all
画像
-
Western blot - Anti-PAF-R antibody (ab104162)All lanes : Anti-PAF-R antibody (ab104162) at 1 µg/ml
Lane 1 : Molecular weight markers
Lane 2 : A549 membrane fraction at 30 µg
Secondary
All lanes : Anti-rabbit IgG (HRP) conjugate at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (8)
ab104162 は 8 報の論文で使用されています。
- Yang J et al. Neuronal extracellular vesicle derived miR-98 prevents salvageable neurons from microglial phagocytosis in acute ischemic stroke. Cell Death Dis 12:23 (2021). PubMed: 33414461
- Sudaryatma PE et al. Bovine Respiratory Syncytial Virus Enhances the Adherence of Pasteurella multocida to Bovine Lower Respiratory Tract Epithelial Cells by Upregulating the Platelet-Activating Factor Receptor. Front Microbiol 11:1676 (2020). PubMed: 32849350
- Bhalla M et al. A1 adenosine receptor signaling reduces Streptococcus pneumoniae adherence to pulmonary epithelial cells by targeting expression of platelet-activating factor receptor. Cell Microbiol 22:e13141 (2020). PubMed: 31709673
- Liu G et al. Platelet activating factor receptor regulates colitis-induced pulmonary inflammation through the NLRP3 inflammasome. Mucosal Immunol 12:862-873 (2019). PubMed: 30976089
- Liang H et al. LncRNA PFL contributes to cardiac fibrosis by acting as a competing endogenous RNA of let-7d. Theranostics 8:1180-1194 (2018). PubMed: 29464008
- Patel PS & Kearney JF CD36 and Platelet-Activating Factor Receptor Promote House Dust Mite Allergy Development. J Immunol 199:1184-1195 (2017). PubMed: 28667161
- Yu Y et al. The expression of platelet-activating factor receptor modulates the cisplatin sensitivity of ovarian cancer cells: a novel target for combination therapy. Br J Cancer N/A:N/A (2014). WB . PubMed: 24921917
- Yuan D et al. Enhanced permeability responses to inflammation in streptozotocin-induced diabetic rat venules: Rho-mediated alterations of actin cytoskeleton and VE-cadherin. Am J Physiol Heart Circ Physiol 307:H44-53 (2014). PubMed: 24778164