Anti-PAEP/Glycodelin 抗体 [EPR23251-87] - BSA and Azide free (ab270524)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAEP/Glycodelin antibody [EPR23251-87] - BSA and Azide free (ab270524)](https://www.abcam.co.jp/ps/products/270/ab270524/Images/ab270524-381959-AntiPAEP-Glycodelin-antibody-EPR2325187-ihc-human-endometrium-tissue.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAEP/Glycodelin antibody [EPR23251-87] - BSA and Azide free (ab270524)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23251-87] to PAEP/Glycodelin - BSA and Azide free
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
製品の概要
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製品名
Anti-PAEP/Glycodelin antibody [EPR23251-87] - BSA and Azide free
PAEP/Glycodelin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23251-87] to PAEP/Glycodelin - BSA and Azide free -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P Human
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免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: K562 cell lysate and human placenta lysate. IHC-P: Human endometrium tissue. Flow: K562 cells.
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特記事項
ab270524 is the carrier-free version of ab270454. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab270524 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23251-87 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| Flow Cyt |
Human
|
| IHC-P |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
|
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Flow Cyt |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
Flow Cyt
Use at an assay dependent concentration. |
ターゲット情報
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機能
This protein is, quantitatively, the main protein synthesized and secreted in the endometrium from mid-luteal phase of the menstrual cycle and during the first semester of pregnancy. -
配列類似性
Belongs to the calycin superfamily. Lipocalin family. -
翻訳後修飾
At least two differentially glycosylated forms are found. Glycodelin-A (GdA) (amniotic fluid) with contraceptive and immunosuppressive activities and glycodelin-S (Gds) (seminal plasma) whose role is not yet known. The gender-specific glycosylation may serve to regulate key process involved in human reproduction. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 5047 Human
- Omim: 173310 Human
- SwissProt: P09466 Human
- Unigene: 532325 Human
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別名
- Alpha uterine protein antibody
- gD antibody
- GdA antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAEP/Glycodelin antibody [EPR23251-87] - BSA and Azide free (ab270524)
Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling PAEP/Glycodelin with ab270454 at 1/500 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in glandular epithelial cells of human endometrium is observed. The section was incubated with ab270454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270454).
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![Flow Cytometry - Anti-PAEP/Glycodelin antibody [EPR23251-87] - BSA and Azide free (ab270524)](https://www.abcam.co.jp/ps/products/270/ab270524/Images/ab270524-381960-AntiPAEP-Glycodelin-antibody-EPR2325187-flow-562-cells.jpg)
Flow Cytometry - Anti-PAEP/Glycodelin antibody [EPR23251-87] - BSA and Azide free (ab270524)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized K-562 (Human chronic myelogenous leukemia lymphoblast) cells treated with 2ng/ml TPA for 32h, then together with 300ng/ml BFA for another 16h (Red) / Untreated control (Green) labeling PAEP/Glycodelin with ab270454 at 1/500 dilution (0.1μg) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. The expression level of PAEP/Glycodelin is induced in TPA treated K-562 cell (PMID:8025274).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270454).
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![Anti-PAEP/Glycodelin antibody [EPR23251-87] - BSA and Azide free (ab270524)](https://www.abcam.co.jp/ps/products/270/ab270524/Images/ab270524-3-benefits-of-recombinant-antibodies.png)
Anti-PAEP/Glycodelin antibody [EPR23251-87] - BSA and Azide free (ab270524)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
Certificate of Compliance
参考文献 (0)
ab270524 は論文での使用が確認できていません。
