Application
Flow Cytometry
Sample
Human Cell (Peripheral blood mononuclear cells)
Specification
Peripheral blood mononuclear cells
Preparation
Cell harvesting/tissue preparation method: Normal human peripheral blood monocytes cultured with M-CSF. Cells were harvested on day 3, non-specific binding was blocked with human Ig, staining was performed on permeabilized cells
Sample buffer: flow cytometery staining buffer (PBS 0.1% sodium azide 1% BSA) after permeabilization
Sample buffer: flow cytometery staining buffer (PBS 0.1% sodium azide 1% BSA) after permeabilization
Fixation
Caltag Fix Perm Kit
Permeabilization
Yes - Caltag Fix Perm Kit
Gating Strategy
Myeloid gate
Other product details
Dilution
1/10
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: PBS 0.1% sodium azide 1% BSA
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG1 alexa488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Isotype matched control: Purified Rabbit IgG1
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Frances Santiago-Schwarz
Verified customer
投稿 Jul 01 2009