Anti-PADI2 / PAD2 抗体 (ab16478)
Key features and details
- Rabbit polyclonal to PADI2 / PAD2
- Suitable for: IHC-P, ELISA, ICC/IF, Flow Cyt, WB
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PADI2 / PAD2 antibody
PADI2 / PAD2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PADI2 / PAD2 -
由来種
Rabbit -
特異性
ab16478 recognises a specific 75kDa band corresponding to PADI2, which is specifically blocked using the immunizing peptide in human colon, skeletal muscle and kidney lysates. There is a non-specific 18kDa band present in skeletal muscle lysates, which is attributed to cross-reactivity of the PADI2 antibody
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アプリケーション
適用あり: IHC-P, ELISA, ICC/IF, Flow Cyt, WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide corresponding to Human PADI2/ PAD2 aa 100-200 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab17091) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16478の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration. PubMed: 19085382
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| Flow Cyt | (1) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 76 kDa).
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| 特記事項 |
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IHC-P
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. PubMed: 19085382 |
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ICC/IF
Use a concentration of 5 µg/ml. |
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Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 76 kDa). |
ターゲット情報
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機能
Catalyzes the deimination of arginine residues of proteins. -
配列類似性
Belongs to the protein arginine deiminase family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 11240 Human
- Entrez Gene: 29511 Rat
- Omim: 607935 Human
- SwissProt: Q9Y2J8 Human
- SwissProt: P20717 Rat
- Unigene: 33455 Human
- Unigene: 98523 Rat
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別名
- KIAA0994 antibody
- OTTHUMP00000044625 antibody
- PAD 2 antibody
see all
画像
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Western blot - Anti-PADI2 / PAD2 antibody (ab16478)Anti-PADI2 / PAD2 antibody (ab16478) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab16478 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution. The 75-kDa band observed is consistent with what has been described in the literature (PMID:18668562; 20668670; 16723463).
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Immunocytochemistry/ Immunofluorescence - Anti-PADI2 / PAD2 antibody (ab16478)ICC/IF image of ab16478 stained human Hek293 cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16478, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab16478 は 14 報の論文で使用されています。
- Casanova V et al. Citrullination Alters the Antiviral and Immunomodulatory Activities of the Human Cathelicidin LL-37 During Rhinovirus Infection. Front Immunol 11:85 (2020). PubMed: 32117246
- Swarup V et al. Identification of Conserved Proteomic Networks in Neurodegenerative Dementia. Cell Rep 31:107807 (2020). PubMed: 32579933
- Gao BS et al. Peptidyl Arginine Deiminase, Type II (PADI2) Is Involved in Urothelial Bladder Cancer. Pathol Oncol Res N/A:N/A (2019). PubMed: 31267364
- García-Berrocoso T et al. Single Cell Immuno-Laser Microdissection Coupled to Label-Free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia. Mol Cell Proteomics 17:175-189 (2018). PubMed: 29133510
- Zhou Y et al. Spontaneous Secretion of the Citrullination Enzyme PAD2 and Cell Surface Exposure of PAD4 by Neutrophils. Front Immunol 8:1200 (2017). PubMed: 28993780