Paclitaxel, Anticancer agent (ab120143)
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- 文献 (5)
製品の概要
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製品名Paclitaxel, Anticancer agent
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製品の詳細Anticancer agent
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別名
- Taxol
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生理活性の詳細
Anticancer agent. Inhibits depolymerization of microtubules, causing mitotic arrest in cancer cells, and apoptosis.
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精製度> 98%
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CAS 番号33069-62-4
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構造式
製品の特性
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体系名5β,20-Epoxy-1,2α,4,7β,10β,13α-hexahydroxytax-11-en-9-one 4,10-diacetate 2-benzoate 13-ester with (2R,3S)-N-benzoyl-3-phenylisoserine
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分子量853.91
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分子式C47H51NO14
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PubChem 登録番号36314
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保存方法Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
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溶解性Soluble in DMSO to 100 mM and in ethanol to 25 mM
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使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Note: Some researchers have found solubility issues in PBS. The recommended procedure (from researcher feedback) for successful solubilisation is as follows: Solubilise 10 mg of ab120143 in 1.25 mL ethanol absolute, mix well, add 1.25mL Cremophor, homogenise. Store at -20°C and after 24 h, defreeze quickly and solubilise in PBS, no crystallisation observed. Please note, Abcam has not yet tested this method.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記O=C(N[C@@H](c1ccccc1)[C@@H](O)C(=O)O[C@H]5C[C@@]6(O)[C@@H](OC(=O)c2ccccc2)[C@H]3[C@@](C)([C@@H](O)C[C@H]4OC[C@@]34OC(C)=O)C(=O)[C@H](OC(C)=O)C(=C5C)C6(C)C)c7ccccc7
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由来
Synthetic
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研究分野
画像
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Functional Studies - Paclitaxel, Anticancer agent (ab120143)Dong et al PLoS One. 2013; 8(12): e83519.E2 inhibits Paclitaxel induced androgen-independent prostate cancer cell death.
(A-D) 100 nM of E2 was added to the media of (A and B) LNCaP and (C and D) PC3 cells for 96h, followed by addition of Paclitaxel at the indicated concentrations for 24h. The cells were stained with Hoechst 33258 (5 µg/ml) to visualize nuclei and propidium iodide (PI) (0.2 µg/ml) to detect membrane damage (B and D). Cell death was quantified by scoring the number of PI positive cells relative to the total number cell nuclei in the same visual field (A and C). The values represent the mean ± S.E. of at least three independent experiments. * denotes p<0.05, ** denotes p<0.01, and *** denotes p<0.001.
E2 = 17-β-estradiol
(From Figure 1 of Dong et al).
参考文献
This product has been referenced in:
- Miller ML & Ojima I Chemistry and chemical biology of taxane anticancer agents. Chem Rec 1:195-211 (2001). Read more (PubMed: 11895119) »
- Wang TH et al. Paclitaxel-induced cell death: where the cell cycle and apoptosis come together. Cancer 88:2619-28 (2000). Read more (PubMed: 10861441) »
- Kuhn JG Pharmacology and pharmacokinetics of paclitaxel. Ann Pharmacother 28:S15-7 (1994). Read more (PubMed: 7915153) »
