Anti-P97/DAP5 抗体 (ab97302)
Key features and details
- Rabbit polyclonal to P97/DAP5
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-P97/DAP5 antibody
P97/DAP5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to P97/DAP5 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Cow -
免疫原
Recombinant fragment corresponding to Human P97/DAP5 aa 1-204.
Database link: P78344 -
ポジティブ・コントロール
- WB: A431 whole cell lysate. Mouse brain whole cell lysate. IHC-P: Cal27 xenograft, human skeletal muscle. Mouse hind brain tissue. IF/ICC: HeLa cell line.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97302の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
|
|
WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 102 kDa.
|
IHC-P |
1/100 - 1/1000.
|
特記事項 |
---|
ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/3000. Predicted molecular weight: 102 kDa. |
IHC-P
1/100 - 1/1000. |
ターゲット情報
-
機能
Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases. -
組織特異性
Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney. -
配列類似性
Belongs to the eIF4G family.
Contains 1 MI domain.
Contains 1 MIF4G domain.
Contains 1 W2 domain. -
翻訳後修飾
Phosphorylation; hyperphosphorylated during mitosis. - Information by UniProt
-
参照データベース
- Entrez Gene: 286870 Cow
- Entrez Gene: 1982 Human
- Entrez Gene: 13690 Mouse
- Entrez Gene: 361628 Rat
- Omim: 602325 Human
- SwissProt: P78344 Human
- SwissProt: Q62448 Mouse
- SwissProt: P79398 Rabbit
see all -
別名
- DAP-5 antibody
- DAP5 antibody
- Death associated protein 5 antibody
see all
画像
-
Anti-P97/DAP5 antibody (ab97302) at 1/500 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 102 kDa5% SDS PAGE
-
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft using ab97302 at 1/100 dilution
-
HeLa cells stained for EIF4G2 (green) using ab97302 at 1/500 dilution in ICC/IF.
-
Paraffin embedded mouse hind brain tissue stained for DAP5 using ab97302 at 1/500 dilution in immunohistochemical analysis.
-
Anti-P97/DAP5 antibody (ab97302) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 102 kDa
7.5% SDS PAGE -
ICC/IF image of ab97302 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97302, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Paraffin embedded human skeletal muscle tissue stained for DAP5 using ab97302 at 10 μg/ml in immunohistochemical analysis.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab97302 は 1 報の論文で使用されています。
- Li S et al. MiR-144-3p-mediated dysregulation of EIF4G2 contributes to the development of hepatocellular carcinoma through the ERK pathway. J Exp Clin Cancer Res 40:53 (2021). PubMed: 33526055