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Anti-p75 NGF Receptor 抗体 [NORI138B] (ab271289)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)
  • Western blot - Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)
  • Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [NORI138B] to p75 NGF Receptor
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-p75 NGF Receptor antibody [NORI138B]
    p75 NGF Receptor 一次抗体 製品一覧

  • 製品の詳細

    Rat monoclonal [NORI138B] to p75 NGF Receptor

  • 由来種

    Rat

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • 免疫原

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • ポジティブ･コントロール

    • WB: Human brain, Neuro-2a, Mouse brain and Rat brain, PC-12 lysates. IHC-P: Human tonsil tissue.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-P
Human
WB
Mouse
Rat
Human
アプリケーション Abreviews 特記事項
WB
1/1000. Predicted molecular weight: 45 kDa.
IHC-P
1/1000.
特記事項
WB
1/1000. Predicted molecular weight: 45 kDa.
IHC-P
1/1000.

ターゲット情報

  • 機能

    Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.

  • 配列類似性

    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.

  • ドメイン

    Death domain is responsible for interaction with RANBP9.
    The extracellular domain is responsible for interaction with NTRK1.

  • 翻訳後修飾

    N- and O-glycosylated.
    O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
    Phosphorylated on serine residues.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CD271 antibody
    • CD271 antigen antibody
    • Gp80 LNGFR antibody
    • Gp80-LNGFR antibody
    • Low affinity nerve growth factor receptor antibody
    • Low affinity neurotrophin receptor p75NTR antibody
    • Low-affinity nerve growth factor receptor antibody
    • Nerve growth factor receptor antibody
    • Nerve growth factor receptor TNFR superfamily member 16 antibody
    • NGF receptor antibody
    • Ngfr antibody
    • p75 ICD antibody
    • p75 Neurotrophin receptor antibody
    • p75 NTR antibody
    • p75(NTR) antibody
    • p75NTR antibody
    • TNFR Superfamily Member 16 antibody
    • TNFRSF16 antibody
    • TNR16_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 16 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling p75 NGF with ab271289 at 1/1000 dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on the germinal center and basement membrane on the epithelium in the human tonsil. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0)

  • Western blot - Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)
    Western blot - Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)
    All lanes : Anti-p75 NGF Receptor antibody [NORI138B] (ab271289) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Rat brain tissue lysate
    Lane 5 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 45 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST 

    Exposure time: lane 1, 2 : 3 minutes lane 3-5 : 59 seconds

  • Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)
    Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab271289 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab271289 は論文での使用が確認できていません。

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