Anti-p75 NGF Receptor 抗体 [NGFR5] (ab3125)

Reviews (5)Q&A (9)References (22)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)
  • Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)

Key features and details

  • Mouse monoclonal [NGFR5] to p75 NGF Receptor
  • Suitable for: ICC, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

APC PE PE/Cy7® PerCP/Cy5.5®

製品の概要

  • 製品名

    Anti-p75 NGF Receptor antibody [NGFR5]
    p75 NGF Receptor 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [NGFR5] to p75 NGF Receptor

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ICC, IHC-Pmore details
    適用なし: WB

  • 種交差性

    交差種: Rat, Human
    交差が予測される動物種: Rabbit, Cat, Baboon非交差種: Mouse

  • 免疫原

    Full length native protein (purified) corresponding to p75 NGF Receptor. Purified from A875 melanoma cell line.

  • エピトープ

    Amino acids 1 - 160.

  • ポジティブ･コントロール

    • IHC-P: Human testis tissue. ICC/IF: PC-12 cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3125の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
Use a concentration of 5 µg/ml.
IHC-P (3)
Use a concentration of 1 - 2 µg/ml.
特記事項
ICC
Use a concentration of 5 µg/ml.
IHC-P
Use a concentration of 1 - 2 µg/ml.
追加情報
Is unsuitable for WB.

ターゲット情報

  • 機能

    Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.

  • 配列類似性

    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.

  • ドメイン

    Death domain is responsible for interaction with RANBP9.
    The extracellular domain is responsible for interaction with NTRK1.

  • 翻訳後修飾

    N- and O-glycosylated.
    O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
    Phosphorylated on serine residues.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CD271 antibody
    • CD271 antigen antibody
    • Gp80 LNGFR antibody
    • Gp80-LNGFR antibody
    • Low affinity nerve growth factor receptor antibody
    • Low affinity neurotrophin receptor p75NTR antibody
    • Low-affinity nerve growth factor receptor antibody
    • Nerve growth factor receptor antibody
    • Nerve growth factor receptor TNFR superfamily member 16 antibody
    • NGF receptor antibody
    • Ngfr antibody
    • p75 ICD antibody
    • p75 Neurotrophin receptor antibody
    • p75 NTR antibody
    • p75(NTR) antibody
    • p75NTR antibody
    • TNFR Superfamily Member 16 antibody
    • TNFRSF16 antibody
    • TNR16_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 16 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue labelling p75 NGF Receptor with ab3125 at 2 µg/mL.

  • Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)
    Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)

    ICC/IF image of ab3125 stained PC-12 (Rat adrenal gland pheochromocytoma cell line) cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3125, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献 (22)

ab3125 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3125 は 22 報の論文で使用されています。

  • Zhang Y  et al. Combination of olfactory ensheathing cells and human umbilical cord mesenchymal stem cell-derived exosomes promotes sciatic nerve regeneration. Neural Regen Res 15:1903-1911 (2020). PubMed: 32246639
  • Castle MJ  et al. Postmortem Analysis in a Clinical Trial of AAV2-NGF Gene Therapy for Alzheimer's Disease Identifies a Need for Improved Vector Delivery. Hum Gene Ther 31:415-422 (2020). PubMed: 32126838
  • Brokhman I  et al. Dual embryonic origin of the mammalian enteric nervous system. Dev Biol 445:256-270 (2019). PubMed: 30472119
  • Du Y  et al. Intracellular Notch1 Signaling in Cancer-Associated Fibroblasts Dictates the Plasticity and Stemness of Melanoma Stem/Initiating Cells. Stem Cells 37:865-875 (2019). PubMed: 30941836
  • Rice FL  et al. The evolution and multi-molecular properties of NF1 cutaneous neurofibromas originating from C-fiber sensory endings and terminal Schwann cells at normal sites of sensory terminations in the skin. PLoS One 14:e0216527 (2019). PubMed: 31107888
View all Publications for this product

レビューと Q&A

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1-10 of 14 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-p75 NGF Receptor antibody [NGFR5]

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (neural precursor)
Permeabilization
No
Specification
neural precursor
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

投稿 Sep 13 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-p75 NGF Receptor antibody [NGFR5]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (Spermatogonial stem cells differentiating into gli)
Permeabilization
Yes - 0.1 % Triton-X
Specification
Spermatogonial stem cells differentiating into gli
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Dominic Schomberg

Verified customer

投稿 Apr 18 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p75 NGF Receptor antibody [NGFR5]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Bone Marrow)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Specification
Bone Marrow
Blocking step
Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF, 10% Formic Acid Decalcification
Read More

Premier Laboratory

Verified customer

投稿 Mar 29 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p75 NGF Receptor antibody [NGFR5]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Melanoma)
Specification
Melanoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH9.0
Permeabilization
Yes - 0.25%Triton X-100
Blocking step
DakoCytomation X0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Read More

Hongwei Shao

Verified customer

投稿 Nov 09 2011

Question

I was wondering if I could get a sample of two antibodies to test this method once before I purchase a large amount of antibody. The two antibodies for which I would like samples are as follows: Anti-Metallothionein antibody [UC1MT] (ab12228) Anti-p75 NGF Receptor antibody [NGFR5] (ab3125).

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Answer

We do not have any free samples or small samples of any of our antibodies but both ab12228 and ab3125 will be covered by our guarantee for one year if you plan on using them for flow cytometry with samples that are one of the species listed on the datasheets. (Human, ferret, and monkey are listed on the ab3125 datasheet and human, mouse, rat and several others on the ab12228 datasheet).

Note that metallothionein is cytoplasmic, so you will not be able to sort live cells. Antibody ab3125 anti-p75 NGF Receptor, on the other hand, recognizes an extracellular epitope, so you should be able to sort live cells.

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Question

Bonjour,
J'ai utilisé le kit ab64261 pour le NGF (6199) et le TrkA (37837).

pour les photo, nous avons un problème avec notre appareil. Pour le moment cela nous est impossible. C'est pour cela que je voulais vous envoyer les lames.

Salutations

Read More
Answer

Merci pour votre émail. Je suis désolée que nous pouvons par regarder les images du coup. Nous sommes en Angleterre, et je pense que ça va devenir compliqué pour envoyer les lames.

Je vais chercher pour trouver des images de mon coté et je vous tiens au courant. Est-ce que en attendant, vous pouvez me confirmerles pointssuivants:

1.)que ces bien des tissus d'origine humaine que vous avez marqué? Ou c'est des tissus d'origine de souris?

2.) C'est quoi comme sérum que vous avez utilisé pour bloquer les lames?

3.) A votre avis, le problème vient de quel réactif?

Je me réjouis de votre réponse. Veuillez s'il vous plait aussi me confirmer comment vous souhaitez procéder.

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Question

Hi there,I am currently working with Cd271 selected MSCs derived from bone marrow. My experiments now require a Cd271 antibody as well as a CD271 primer for qPCR. I cannot see these products on your website and therefore wondering if you still supply these. I was recommended to try this company by acolleaguebut I'm not having much luck finding these on your site.Any information would be greatly appreciated.

Read More
Answer

Thank you for your inquiry.

I am pleased to let you know that we currently have a number of CD271 antibodies (24 in total); we sell them under the alternative name 'p75-NGF receptor', such as the rabbit polyclonal antibody ab8874 and the mouse monoclonal ab8877 (as can be seen on each datasheet in the section 'Alternative names'):
Click here (or use the following: https://www.abcam.com/p75-NGF-Receptor-antibody-ab8874.html).
Click here (or use the following: https://www.abcam.com/p75-NGF-Receptor-antibody-ME204-ab8877.html).

These antibodies are guaranteed to work with mouse and human samples (amongst others) and in a number of applications. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase (www.abcam.com/Abpromise).

If you are looking for other specifications (e.g. other species or applications), I would be pleased to assist you further in finding the appropriate antibody.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews

We do not have many qPCR primer in our catalog. We have primer for common controls such as GAPDH (ab83588) available, but CD271/p75-NGF receptor is unfortunately not in this selection.

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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Question

Bonjour, J'ai effectué de nouveaux tests en augmentant la concentration. J'ai obtenu un marquage pour NGF et TrkA fort mais avec beaucoup de fond. La p75 est toujours faible, donc je vais la concentré plus. Pour repondre à votre question, j'ai bien reçu les 3 anticorps dans le même colis. Merci pour votre aide Meilleures salutations

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Answer

Merci pour ces précisions. N'hésitez pas à nous contacter de nouveau si vous rencontrez des difficultés.  

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Question

Bonjour, J'ai effectué les tests pour les 3 anticorps avec les paramètres que vous m'aviez conseillé. Je n'ai malheureusement pas de resultats très concluant. Je vais effectuer un test en concentrant plus les anticorps. Est ce que le tissu utilisé pour les les tests est le plus apropriez ? Merci d'avance pour votre aide. Meilleures salutations

Read More
Answer

Le tissu cérébral humain est en effet un tissu de choix pour l'expression de TrkA, NGF et le récepteur p75 NGF. Avez-vous reçu ces 3 anticorps en même temps (même colis de livraison)?  

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Question

Bonjour, nous avons bien reçu vos anticorps, merci. J'ai effectué un premier test qui n'a malheureusement pas fonctionné. Voici les paramètres: NGF (ab 6199): Pas de prétraitement, dilution a 1/500 incubé 30 minute, utilisations du système ENVISION/HRP de Dako pour anticorps rabbit polyclonal, utilisation PBS comme washbuffer. TRKa (ab 37837): Paramètres identique que ci-dessus, dilution à 1/100 P75 (ab 3125): Pré traitement à la chaleur de 3*5 minutes au Micro-ondes à 600w dans tampon citrate a pH 6, dilution à 1/200 incubé pendant 30 minutes, utilisation de système ENVISION/HRP de Dako pour Mouse monoclonal antibody, utiisaion de PBS. J'aimerais savoir si en utilisant de TBS plutôt que du PBS cela fonctionnerait mieux? Et si je dois procéder à un prétraitement pour le NGF et le TrkA ? Le test à été effectué sur du tissus cérébral humain. Merci d'avance pour votre réponse et meilleures salutations.

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Answer

Merci de nous avoir contactés. Je suis désolé d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab3125, ab6199, ab37837 en IHC-P. Notre politique est d'offrir tout d'abord la meilleure assistance technique. Si nous concluons que le produit que vous avez reçu ne fonctionne pas comme mentionné sur sa fiche technique et que ce produit a été commandé sous 6 mois, nous nous ferons un plaisir de vous offrir un remplacement gratuit ou un avoir. J'aimerais donc vous offrir quelques suggestions afin d'optimiser vos résultats. - Pour les 3 anticorps je recommanderais une incubation sur la nuit à 4°C (ou même à température ambiante) en prenant soin que les échantillons ne soient pas sujet à évaporation. Pour les dilutions des anticorps primaires, se reporter aux fiches techniques. - Pour les 3 anticorps je recommanderais également un démasquage de l'antigène à haute température en utilisant du tampon Tris EDTA pH 9,0. Pourriez-vous me communiquer quelques détails concernant la préparation des échantillons (agent et temps de fixation)? Les anticorps secondaires sont-il compatibles? Sont-ils bons? J'espère que ces informations vous aideront. Si les suggestions ne permettent pas d'améliorer vos résultats, n'hésitez pas à me recontacter pour plus d'assistance.

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1-10 of 14 Abreviews or Q&A

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