Anti-p75 NGF Receptor 抗体 [NGFR5] (ab3125)
Key features and details
- Mouse monoclonal [NGFR5] to p75 NGF Receptor
- Suitable for: ICC, IHC-P
- Reacts with: Rat
- Isotype: IgG1
製品の概要
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製品名
Anti-p75 NGF Receptor antibody [NGFR5]
p75 NGF Receptor 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [NGFR5] to p75 NGF Receptor -
由来種
Mouse -
アプリケーション
適用あり: ICC, IHC-Pmore details
適用なし: WB -
種交差性
交差種: Rat
交差が予測される動物種: Rabbit, Cat, Baboon非交差種: Mouse
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免疫原
NGFR from A875 melanoma cells.
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エピトープ
Amino acids 1 - 160. -
ポジティブ・コントロール
- IHC: Human testis tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Constituents: 0.0268% PBS, 0.2% BSA -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
NGFR5 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
- PE Anti-p75 NGF Receptor antibody [NGFR5], prediluted (ab157333)
- APC Anti-p75 NGF Receptor antibody [NGFR5], prediluted (ab176522)
- Anti-p75 NGF Receptor antibody [NGFR5] - BSA and Azide free (ab212682)
- PE/Cy7® Anti-p75 NGF Receptor antibody [NGFR5] (ab234270)
- PerCP/Cy5.5® Anti-p75 NGF Receptor antibody [NGFR5] (ab234446)
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3125の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use a concentration of 5 µg/ml.
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IHC-P | (3) |
Use a concentration of 1 - 2 µg/ml.
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特記事項 |
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ICC
Use a concentration of 5 µg/ml. |
IHC-P
Use a concentration of 1 - 2 µg/ml. |
ターゲット情報
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機能
Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. -
配列類似性
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
ドメイン
Death domain is responsible for interaction with RANBP9.
The extracellular domain is responsible for interaction with NTRK1. -
翻訳後修飾
N- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
Phosphorylated on serine residues. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 101101519 Cat
- Entrez Gene: 100349095 Rabbit
- Entrez Gene: 24596 Rat
- SwissProt: P07174 Rat
- Unigene: 10980 Rat
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別名
- CD271 antibody
- CD271 antigen antibody
- Gp80 LNGFR antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue labelling p75 NGF Receptor with ab3125 at 2 µg/mL.
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ICC/IF image of ab3125 stained PC-12 (Rat adrenal gland pheochromocytoma cell line) cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3125, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (19)
ab3125 は 19 報の論文で使用されています。
- Du Y et al. Intracellular Notch1 Signaling in Cancer-Associated Fibroblasts Dictates the Plasticity and Stemness of Melanoma Stem/Initiating Cells. Stem Cells 37:865-875 (2019). PubMed: 30941836
- Rice FL et al. The evolution and multi-molecular properties of NF1 cutaneous neurofibromas originating from C-fiber sensory endings and terminal Schwann cells at normal sites of sensory terminations in the skin. PLoS One 14:e0216527 (2019). PubMed: 31107888
- Foerster Y et al. Relevance of Neurotrophin Receptors CD271 and TrkC for Prognosis, Migration, and Proliferation in Head and Neck Squamous Cell Carcinoma. Cells 8:N/A (2019). PubMed: 31569361
- Brokhman I et al. Dual embryonic origin of the mammalian enteric nervous system. Dev Biol 445:256-270 (2019). PubMed: 30472119
- Li Y et al. ROCK Inhibitor Y27632 Induced Morphological Shift and Enhanced Neurite Outgrowth-Promoting Property of Olfactory Ensheathing Cells via YAP-Dependent Up-Regulation of L1-CAM. Front Cell Neurosci 12:489 (2018). PubMed: 30618636