Anti-p75 NGF Receptor 抗体 [MC-192] (ab6172)

Reviews (2)Q&A (6)References (10)
Immunocytochemistry - Anti-p75 NGF Receptor antibody [MC-192] (ab6172)

    Key features and details

    • Mouse monoclonal [MC-192] to p75 NGF Receptor
    • Suitable for: ICC
    • Reacts with: Rat
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-p75 NGF Receptor antibody [MC-192]
      p75 NGF Receptor 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [MC-192] to p75 NGF Receptor

    • 由来種

      Mouse

    • アプリケーション

      適用あり: ICCmore details

    • 種交差性

      交差種: Rat

    • 免疫原

      Rat PC-12 cells.

    • ポジティブ･コントロール

      • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC12.

    • 特記事項


      A well characterised and versatile monoclonal antibody, clone number MC-192, that is of central importance in the study of LANGF (p75-NGF) receptor.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab6172 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    ICC Use at an assay dependent concentration.

    ターゲット情報

    • 機能

      Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.

    • 配列類似性

      Contains 1 death domain.
      Contains 4 TNFR-Cys repeats.

    • ドメイン

      Death domain is responsible for interaction with RANBP9.
      The extracellular domain is responsible for interaction with NTRK1.

    • 翻訳後修飾

      N- and O-glycosylated.
      O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
      Phosphorylated on serine residues.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • CD271 antibody
        • CD271 antigen antibody
        • Gp80 LNGFR antibody
        • Gp80-LNGFR antibody
        • Low affinity nerve growth factor receptor antibody
        • Low affinity neurotrophin receptor p75NTR antibody
        • Low-affinity nerve growth factor receptor antibody
        • Nerve growth factor receptor antibody
        • Nerve growth factor receptor TNFR superfamily member 16 antibody
        • NGF receptor antibody
        • Ngfr antibody
        • p75 ICD antibody
        • p75 Neurotrophin receptor antibody
        • p75 NTR antibody
        • p75(NTR) antibody
        • p75NTR antibody
        • TNFR Superfamily Member 16 antibody
        • TNFRSF16 antibody
        • TNR16_HUMAN antibody
        • Tumor necrosis factor receptor superfamily member 16 antibody
        see all

      画像

      • Immunocytochemistry - Anti-p75 NGF Receptor antibody [MC-192] (ab6172)
        Immunocytochemistry - Anti-p75 NGF Receptor antibody [MC-192] (ab6172)

        ab6172 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab6172 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      参考文献 (10)

      ab6172 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab6172 は 10 報の論文で使用されています。

      • Niapour N  et al. An efficient system for selection and culture of Schwann cells from adult rat peripheral nerves. Cytotechnology 68:629-36 (2016). PubMed: 25680695
      • Nelson AR  et al. Noradrenergic sympathetic sprouting and cholinergic reinnervation maintains non-amyloidogenic processing of AßPP. J Alzheimers Dis 38:867-79 (2014). IHC ; Rat . PubMed: 24081376
      • Barres BA Designing and troubleshooting immunopanning protocols for purifying neural cells. Cold Spring Harb Protoc 2014:1342-7 (2014). PubMed: 25447277
      • Eleftheriadou I  et al. Specific retrograde transduction of spinal motor neurons using lentiviral vectors targeted to presynaptic NMJ receptors. Mol Ther 22:1285-98 (2014). PubMed: 24670531
      • Masaeli E  et al. Fabrication, characterization and cellular compatibility of poly(hydroxy alkanoate) composite nanofibrous scaffolds for nerve tissue engineering. PLoS One 8:e57157 (2013). IF . PubMed: 23468923
      • Graber DJ & Harris BT Purification and culture of spinal motor neurons from rat embryos. Cold Spring Harb Protoc 2013:319-26 (2013). PubMed: 23547162
      • Song W  et al. Mutant SOD1G93A triggers mitochondrial fragmentation in spinal cord motor neurons: neuroprotection by SIRT3 and PGC-1a. Neurobiol Dis 51:72-81 (2013). PubMed: 22819776
      • Niapour A  et al. Cotransplantation of human embryonic stem cell-derived neural progenitors and schwann cells in a rat spinal cord contusion injury model elicits a distinct neurogenesis and functional recovery. Cell Transplant 21:827-43 (2012). PubMed: 21944670
      • Niapour A  et al. Novel method to obtain highly enriched cultures of adult rat Schwann cells. Biotechnol Lett 32:781-6 (2010). ICC/IF ; Rat . PubMed: 20213527
      • Chandler CE  et al. A monoclonal antibody modulates the interaction of nerve growth factor with PC12 cells. J Biol Chem 259:6882-9 (1984). PubMed: 6327698

      レビューと Q&A

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      1-8 of 8 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p75 NGF Receptor antibody [MC-192]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      Serum as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate sodium
      Sample
      Cat Tissue sections (brain stem)
      Specification
      brain stem
      Permeabilization
      Yes - 0.3% triton
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Sep 12 2014

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p75 NGF Receptor antibody [MC-192]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate sodium
      Sample
      Rat Tissue sections (brainstem slice)
      Specification
      brainstem slice
      Permeabilization
      Yes - 0.3% triton
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Sep 12 2014

      Question

      I have a question about the mouse monoclonal antibody to rat p75NGF Receptor [MC-192] (ab6172).

      Could I please ask you for a reference where it is determined whether this antibody recognizes human P75NGFR?

      Read More
      Answer

      Thank you very much for your interest in ab6172.

      To our knowledge, ab6172 has not been tested in human. Therefore, I can offer a discount off a future purchase if you buy ab6172 now, test it inhuman and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

      If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me know that you would like to proceed and test ab6172 in human. I will then send a discount code. This code must be issued before purchasing ab6172 so please wait for my reply before ordering.

      2. Purchase ab6172 either by phone, fax, or online (www.abcam.com).

      3. Test it in human.

      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
      Please remember that submission of the Abreview is sufficient for the discount code to become active.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab6172 turns out to be unsuitable for human, you will still receive the discount on your next purchase after your Abreview has been submitted.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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      Question

      I purchased a vial of LANGF (ab6172). However, while trying to run the staining following your recommendations (1-2 micro grams per ml), I noticed that there is no concentration given for the vial. Thus it is difficult to run the experiment. No-one in the UK or US seem to know the concentration which is a little disturbing and I have been advised to e-mail you. I would be grateful if you could let me know the concentration as soon as impossible as we are working to a deadline and our first batch of slides are now defunct.

      Read More
      Answer

      Thank you for your enquiry. I apologise for any confusion that has been caused with product Ab6172. There is a concentration value cited on the product data sheet, as 1.0mg/ml. You should have received a copy of this data sheet upon receipt of the product. I apologise if this was not the case, it is possible that the data sheet has only been recently updated with this information. Good luck with your research, if you need any further clarification please do not hesitate to get back in touch with me.

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      Question

      Can this antibody be used for human cells?

      Read More
      Answer

      Dear Dr Hilker I am afraid that this antibody only recognises rat Low Affinity Nerve Growth Factor receptor. Regards Michael

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      Question

      would it be possible to obtain this antibody biotinilated. We will by a very big amount of it. What could be the price? Thanks

      Read More
      Answer

      Thank you for your enquiry. This antibody is not currently available biotinylated, however we do offer a conjugation service for £150 pounds sterling or $225 US dollars. We would need to biotinylate at least 1mg antibody; the price listed on our web site (£187.50 pounds sterling or $282 US dollars) is for 0.1mg unlabelled antibody, so the total price of 1mg biotinylated antibody would be £2025 pounds sterling or $3045 US dollars.

      Read More

      Question

      Could this antibody be used for FACS analysis of PC12 cells?

      Read More
      Answer

      Yes, this antibody is ideal for FACS of PC12 cells.

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      Question

      Could this antibody be used formalin fixed and paraffin section ?

      Read More
      Answer

      Yes, the antibody is excellent in these applications.

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