Application
Western blot
Sample
Human Tissue lysate - whole (CSF)
Loading amount
50 µg
Specification
CSF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Anti rabbit HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 90 kDa
Positive control
Choroid plexus
Negative control
Serum
Additional data
Additional Notes
In A and B, red nuclei are staining with anti p73 deltaN antibody (Ab11997). In green, immunostaining with Ab14430.
Abbreviations: ChP, choroid plexus; CSF, cerebrospinal fluid. Numbers in C represents analyzed ages.
Abbreviations: ChP, choroid plexus; CSF, cerebrospinal fluid. Numbers in C represents analyzed ages.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Alfredo Cabrera
Verified customer
投稿 Dec 11 2006