Application
ChIP
Sample
Human Cell lysate - nuclear (human cancer cell lines)
Specification
human cancer cell lines
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step
Real-time PCR
Positive control
stable Saos cell line with inducible overexpression of TAp73alpha and Tap73beta
Negative control
Saos parental cells and Saos cells overexpressing p53
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Additional data
PubMed ID
21459846: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MRS. Daniela Kramer
Verified customer
投稿 May 03 2011