Anti-p73 抗体 (ab14430)
Key features and details
- Rabbit polyclonal to p73
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-p73 antibody
p73 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to p73 -
由来種
Rabbit -
特異性
The antibody is not expected to react with deltaNp73. -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Chimpanzee, Rhesus monkey, Gorilla, African green monkey, Orangutan
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免疫原
Synthetic peptide corresponding to Human p73 aa 1-62. The epitope is found in isoforms alpha, beta, gamma, delta, epsilon, zeta, and kappa of human p73.
Database link: O15350 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antibody was purified using an epitope specific to p73 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14430の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use a concentration of 0.1 µg/ml.
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| WB | (2) |
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 69 kDa.
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| 特記事項 |
|---|
|
IP
Use a concentration of 0.1 µg/ml. |
|
WB
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 69 kDa. |
ターゲット情報
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機能
Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. -
組織特異性
Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue. -
配列類似性
Belongs to the p53 family.
Contains 1 SAM (sterile alpha motif) domain. -
ドメイン
Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain.
The WW-binding motif mediates interaction with WWOX. -
翻訳後修飾
Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity.
Higher levels of phosphorylation seen in the brain from patients with Huntington disease.
Ubiquitinated; leading to its degradation by the proteasome. -
細胞内局在
Nucleus. Accumulates in the nucleus in response to DNA damage. - Information by UniProt
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参照データベース
- Entrez Gene: 7161 Human
- Entrez Gene: 22062 Mouse
- Omim: 601990 Human
- SwissProt: O15350 Human
- SwissProt: Q9JJP2 Mouse
- Unigene: 697294 Human
- Unigene: 706990 Human
- Unigene: 78015 Mouse
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別名
- p53 like transcription factor antibody
- p53 related protein antibody
- p53-like transcription factor antibody
see all
画像
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Western blot - Anti-p73 antibody (ab14430)All lanes : Anti-p73 antibody (ab14430) at 0.1 µg/ml
Lane 1 : HEK293 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : RKO cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 69 kDa
Exposure time: 75 seconds -
Immunoprecipitation - Anti-p73 antibody (ab14430)Immunoprecipitation of p73 with ab14430 at 0.1 µg/mL.
Lane 1 and 2: Different lots of ab14430 + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.Lane 2: IgG control + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Detection: Chemiluminescence
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (19)
ab14430 は 19 報の論文で使用されています。
- Iegiani G et al. CENPE Inhibition Leads to Mitotic Catastrophe and DNA Damage in Medulloblastoma Cells. Cancers (Basel) 13:N/A (2021). PubMed: 33804489
- Cai BH et al. Synergistic activation of the NEU4 promoter by p73 and AP2 in colon cancer cells. Sci Rep 9:950 (2019). PubMed: 30700826
- Sharif T et al. TAp73 Modifies Metabolism and Positively Regulates Growth of Cancer Stem-Like Cells in a Redox-Sensitive Manner. Clin Cancer Res 25:2001-2017 (2019). PubMed: 30593514
- Yao Z et al. DNA Methylation Activates TP73 Expression in Hepatocellular Carcinoma and Gastrointestinal Cancer. Sci Rep 9:19367 (2019). PubMed: 31852961
- Pallavicini G et al. Inactivation of Citron Kinase Inhibits Medulloblastoma Progression by Inducing Apoptosis and Cell Senescence. Cancer Res 78:4599-4612 (2018). PubMed: 29921697