Application
Western blot
Sample
Rat Tissue lysate - whole (liver)
Loading amount
20 µg
Specification
liver
Treatment
AEE788 50mg/kg 24h and 1week
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/250
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%milk
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Dilution
1/5000
Detection
Detection method
ECL
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 70 kDa
Additional data
Additional Notes
Although the company recommends to use BSA as blocking buffer, we are used to use milk as blocking buffer. It works in this case.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 19 2010