Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Turtle Tissue sections (normal skin)
Specification
normal skin
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1mM EDTA pH8 30min
Permeabilization
No
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 27°C
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 27°C · Diluent: 1% BSA in PBS
Secondary antibody
Name
Non-Abcam antibody was used: EnVision+Dual Link System HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 19 2011