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リコンビナントRabMAb

Anti-p60 CAF1/MPP7 抗体 [EPR6105] (ab109442)

Submit a review Submit a question References (5)
Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
  • Flow Cytometry (Intracellular) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
  • Immunoprecipitation - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
  • Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6105] to p60 CAF1/MPP7
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-p60 CAF1/MPP7 antibody [EPR6105]
    p60 CAF1/MPP7 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR6105] to p60 CAF1/MPP7

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IP, IHC-Pmore details

  • 種交差性

    交差種: Human
    非交差種: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Jurkat, HeLa, 293T, and Caco2 cell lysates; IHC: Human colon and tonsil tissues. Flow Cyt: HeLa cells. IP: HeLa.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR6105

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109442の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
WB
1/10000 - 1/50000. Predicted molecular weight: 61 kDa.
IP
1/10 - 1/100.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
WB
1/10000 - 1/50000. Predicted molecular weight: 61 kDa.
IP
1/10 - 1/100.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex.

  • 配列類似性

    Belongs to the WD repeat HIR1 family.
    Contains 7 WD repeats.

  • 発生段階

    Active complex is found in G1, S and G2 phases.

  • 翻訳後修飾

    Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.

  • 細胞内局在

    Nucleus. Cytoplasm. DNA replication foci. Cytoplasmic in M phase.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CAF 1 subunit B antibody
      • CAF 1A antibody
      • CAF I 60 kDa subunit antibody
      • CAF IP60 antibody
      • CAF-1 antibody
      • CAF-1 subunit B antibody
      • CAF-I 60 kDa subunit antibody
      • CAF-I p60 antibody
      • CAF1 antibody
      • CAF1A antibody
      • CAF1B_HUMAN antibody
      • CAF1P60 antibody
      • CHAF 1B antibody
      • CHAF1B antibody
      • Chromatin assembly factor 1 subunit B antibody
      • Chromatin assembly factor I antibody
      • Chromatin assembly factor I p60 subunit antibody
      • Human chromatin assembly factor-I p60 subunit antibody
      • M phase phosphoprotein 7 antibody
      • M-phase phosphoprotein 7 antibody
      • MPHOSPH7 antibody
      • MPP 7 antibody
      • MPP7 antibody
      • p60 subunit antibody
      see all

    画像

    • Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
      Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
      All lanes : Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Caco2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 61 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?

    • Flow Cytometry (Intracellular) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
      Flow Cytometry (Intracellular) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

      Overlay histogram showing HeLa cells stained with ab109442 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109442, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

      ab109442 at 1/250 dilution staining p60 CAF1/MPP7 in paraffin-embedded Human colon tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

      ab109442 at 1/250 dilution staining p60 CAF1/MPP7 in paraffin-embedded Human tonsil tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
      Immunoprecipitation - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

      p60 CAF1/MPP7 

      was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with 109442 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg

      Lane 2: ab109442 IP in HeLa whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab109442 in HeLa whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

       

    • Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
      Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

    参考文献 (5)

    ab109442 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab109442 は 5 報の論文で使用されています。

    • Tachiwana H  et al. Chromatin structure-dependent histone incorporation revealed by a genome-wide deposition assay. Elife 10:N/A (2021). PubMed: 33970102
    • Shen Y  et al. Multi-Omics Integrative Analysis Uncovers Molecular Subtypes and mRNAs as Therapeutic Targets for Liver Cancer. Front Med (Lausanne) 8:654635 (2021). PubMed: 34109194
    • Yang S  et al. CAF-1/p150 promotes cell proliferation, migration, invasion and predicts a poor prognosis in patients with cervical cancer. Oncol Lett 20:2338-2346 (2020). PubMed: 32782551
    • Duan Y  et al. CHAF1B promotes proliferation and reduces apoptosis in 95-D lung cancer cells and predicts a poor prognosis in non-small cell lung cancer. Oncol Rep 41:2518-2528 (2019). PubMed: 30720130
    • Piquet S  et al. The Histone Chaperone FACT Coordinates H2A.X-Dependent Signaling and Repair of DNA Damage. Mol Cell 72:888-901.e7 (2018). PubMed: 30344095

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