Anti-p55/DCAF1 抗体 - ChIP Grade (ab1766)
製品の概要
-
製品名
Anti-p55/DCAF1 antibody - ChIP Grade
p55/DCAF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to p55/DCAF1 - ChIP Grade -
由来種
Rabbit -
アプリケーション
適用あり: ChIP, ICC/IF, IP, WBmore details -
種交差性
交差種: Drosophila melanogaster
交差が予測される動物種: Chicken, Xenopus laevis, Zebrafish
-
免疫原
Synthetic peptide:
CNVYNDEEPEIPASELETNTA
, corresponding to amino acids 411-430 of Drosophilia melanogaster CAF 1 (DCAF 1). -
特記事項
Complex that assembles histone octamers onto replicating DNA in vitro. Caf-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones h3 and h4 to replicating DNA; histones H2a/H2b can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P150 and p60 form complexes with newly synthesized histones h3 and acetylated H4 in cell extracts (by similarity). P55 associates with acetyltransferases and deacetylases in cell extracts and is also a component of the nucleosome remodeling factor complex (NURF), a protein complex consisting of four polypeptides that facilitates the perturbation of chromatin structure in vitro in an ATP-dependent manner. This subunit may assist targeting of protein complexes to chromatin.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Whole antiserum -
一次抗体 備考
Complex that assembles histone octamers onto replicating DNA in vitro. Caf-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones h3 and h4 to replicating DNA; histones H2a/H2b can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P150 and p60 form complexes with newly synthesized histones h3 and acetylated H4 in cell extracts (by similarity). P55 associates with acetyltransferases and deacetylases in cell extracts and is also a component of the nucleosome remodeling factor complex (NURF), a protein complex consisting of four polypeptides that facilitates the perturbation of chromatin structure in vitro in an ATP-dependent manner. This subunit may assist targeting of protein complexes to chromatin. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab1766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ChIP | 1/450. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| WB | 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
ターゲット情報
-
関連性
p55/DCAF1 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 41836 Drosophila melanogaster
- Entrez Gene: 779086 Xenopus laevis
- SwissProt: Q24572 Drosophila melanogaster
- SwissProt: Q07CZ6 Xenopus laevis
-
別名
- Caf 1 antibody
- CAF1 p55 subunit antibody
- chaf1b antibody
see all
画像
-
ChIP - Anti-p55/DCAF1 antibody - ChIP Grade (ab1766)This image is courtesy of an Abreview submitted by Dr Kat MillenChromatin was obtained from Kc167 cells fixed with formaldehyde for 10 min. The ChIP was performed with 30-40 µg of chromatin, ab1766 at 1/450 dilution (incubated with samples for 16h at 4°C) and 20µl of Protein G Agarose beads per ChIP. The immunoprecipitated DNA was quantified by real time PCR (SYBR) . Gene A's enhancer region locus (positive control) shows enrichment when compared with it's coding sequence locus (negative control).
-
Western blot - Anti-p55/DCAF1 antibody - ChIP Grade (ab1766)This image is courtesy of an Abreview submitted by Dr Kat MillenAll lanes : Anti-p55/DCAF1 antibody - ChIP Grade (ab1766) at 1/2000 dilution
Lane 1 : Kc167 cell lysate
Lane 2 : DmD8 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L)-HRP Conjugate at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 1 minute
プロトコール
データシートおよび資料
参考文献
ab1766 は 4 報の論文で使用されています。
- Abascal F et al. Subfunctionalization via adaptive evolution influenced by genomic context: the case of histone chaperones ASF1a and ASF1b. Mol Biol Evol 30:1853-66 (2013). PubMed: 23645555
- Lee H et al. A dual role for the dREAM/MMB complex in the regulation of differentiation-specific E2F/RB target genes. Mol Cell Biol 32:2110-20 (2012). IP, WB ; Drosophila melanogaster . PubMed: 22451490
- Lee H et al. Drosophila RB proteins repress differentiation-specific genes via two different mechanisms. Mol Cell Biol 30:2563-77 (2010). WB ; Drosophila melanogaster . PubMed: 20176807
- Groth A et al. Human Asf1 regulates the flow of S phase histones during replicational stress. Mol Cell 17:301-11 (2005). PubMed: 15664198
