Anti-p55/DCAF1 抗体 - ChIP Grade (ab1766)

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製品の概要

  • 製品名

    Anti-p55/DCAF1 antibody - ChIP Grade
    p55/DCAF1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to p55/DCAF1 - ChIP Grade

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ChIP, ICC/IF, IP, WBmore details

  • 種交差性

    交差種: Drosophila melanogaster
    交差が予測される動物種: Chicken, Xenopus laevis, Zebrafish

  • 免疫原

    Synthetic peptide:

    CNVYNDEEPEIPASELETNTA

    , corresponding to amino acids 411-430 of Drosophilia melanogaster CAF 1 (DCAF 1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項


    Complex that assembles histone octamers onto replicating DNA in vitro. Caf-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones h3 and h4 to replicating DNA; histones H2a/H2b can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P150 and p60 form complexes with newly synthesized histones h3 and acetylated H4 in cell extracts (by similarity). P55 associates with acetyltransferases and deacetylases in cell extracts and is also a component of the nucleosome remodeling factor complex (NURF), a protein complex consisting of four polypeptides that facilitates the perturbation of chromatin structure in vitro in an ATP-dependent manner. This subunit may assist targeting of protein complexes to chromatin.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.02% Sodium azide

  • 精製度

    Whole antiserum

  • 一次抗体 備考

    Complex that assembles histone octamers onto replicating DNA in vitro. Caf-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones h3 and h4 to replicating DNA; histones H2a/H2b can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P150 and p60 form complexes with newly synthesized histones h3 and acetylated H4 in cell extracts (by similarity). P55 associates with acetyltransferases and deacetylases in cell extracts and is also a component of the nucleosome remodeling factor complex (NURF), a protein complex consisting of four polypeptides that facilitates the perturbation of chromatin structure in vitro in an ATP-dependent manner. This subunit may assist targeting of protein complexes to chromatin.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP 1/450.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

ターゲット情報

  • 関連性

    p55/DCAF1 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair.

  • 細胞内局在

    Nuclear

  • 参照データベース

    • 別名

      • Caf 1 antibody
      • CAF1 p55 subunit antibody
      • chaf1b antibody
      • Chromatin assembly factor 1 p55 subunit antibody
      • Chromatin assembly factor 1 p60 subunit antibody
      • chromatin assembly factor 1 subunit antibody
      • dCAF1 antibody
      • NURF 55 antibody
      • p55 antibody
      • Probable histone binding protein Caf1 antibody
      see all

    画像

    • ChIP - Anti-p55/DCAF1 antibody - ChIP Grade (ab1766)
      ChIP - Anti-p55/DCAF1 antibody - ChIP Grade (ab1766)This image is courtesy of an Abreview submitted by Dr Kat Millen

      Chromatin was obtained from Kc167 cells fixed with formaldehyde for 10 min. The ChIP was performed with 30-40 µg of chromatin, ab1766 at 1/450 dilution (incubated with samples for 16h at 4°C) and 20µl of Protein G Agarose beads per ChIP. The immunoprecipitated DNA was quantified by real time PCR (SYBR) . Gene A's enhancer region locus (positive control) shows enrichment when compared with it's coding sequence locus (negative control).

      See Abreview

    • Western blot - Anti-p55/DCAF1 antibody - ChIP Grade (ab1766)
      Western blot - Anti-p55/DCAF1 antibody - ChIP Grade (ab1766)This image is courtesy of an Abreview submitted by Dr Kat Millen
      All lanes : Anti-p55/DCAF1 antibody - ChIP Grade (ab1766) at 1/2000 dilution

      Lane 1 : Kc167 cell lysate
      Lane 2 : DmD8 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (H+L)-HRP Conjugate at 1/2000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 55 kDa
      Observed band size: 55 kDa


      Exposure time: 1 minute

      See Abreview

    参考文献

    This product has been referenced in:

    • Abascal F  et al. Subfunctionalization via adaptive evolution influenced by genomic context: the case of histone chaperones ASF1a and ASF1b. Mol Biol Evol 30:1853-66 (2013). Read more (PubMed: 23645555) »
    • Lee H  et al. A dual role for the dREAM/MMB complex in the regulation of differentiation-specific E2F/RB target genes. Mol Cell Biol 32:2110-20 (2012). IP, WB ; Drosophila melanogaster . Read more (PubMed: 22451490) »
    See all 4 Publications for this product

    ab1766 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    ChIP abreview for Anti-p55/DCAF1 antibody

     Good
    Abreviews
    abreview image
    Application
    ChIP
    Detection step
    Real-time PCR
    Sample
    Fruit fly (Drosophila melanogaster) Cell lysate - nuclear (Kc167 cell line)
    Specification
    Kc167 cell line
    Negative control
    Coding sequences ChIP.
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Positive control
    Gene A enhancer region ChIP.
    Read More

    Dr. Kat Millen

    Verified customer

    投稿 Jul 25 2013

    Western blot abreview for Anti-p55/DCAF1 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Non-reduced Denaturing (4% gel)
    Sample
    Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (DmD8 and Kc167 cell lines)
    Specification
    DmD8 and Kc167 cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More

    Dr. Kat Millen

    Verified customer

    投稿 Jul 17 2013

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