Anti-p55/DCAF1 抗体 (ab1766)

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Key features and details

  • Rabbit polyclonal to p55/DCAF1
  • Suitable for: ICC/IF, IP
  • Reacts with: Drosophila melanogaster
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-p55/DCAF1 antibody
    p55/DCAF1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to p55/DCAF1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, IPmore details

  • 種交差性

    交差種: Drosophila melanogaster
    交差が予測される動物種: Chicken, Xenopus laevis, Zebrafish

  • 免疫原

    Synthetic peptide:

    CNVYNDEEPEIPASELETNTA

    , corresponding to amino acids 411-430 of Drosophilia melanogaster CAF 1 (DCAF 1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項


    Complex that assembles histone octamers onto replicating DNA in vitro. Caf-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones h3 and h4 to replicating DNA; histones H2a/H2b can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P150 and p60 form complexes with newly synthesized histones h3 and acetylated H4 in cell extracts (by similarity). P55 associates with acetyltransferases and deacetylases in cell extracts and is also a component of the nucleosome remodeling factor complex (NURF), a protein complex consisting of four polypeptides that facilitates the perturbation of chromatin structure in vitro in an ATP-dependent manner. This subunit may assist targeting of protein complexes to chromatin.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.02% Sodium azide
  • Concentration information loading...

  • 精製度

    Whole antiserum

  • 一次抗体 備考

    Complex that assembles histone octamers onto replicating DNA in vitro. Caf-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones h3 and h4 to replicating DNA; histones H2a/H2b can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P150 and p60 form complexes with newly synthesized histones h3 and acetylated H4 in cell extracts (by similarity). P55 associates with acetyltransferases and deacetylases in cell extracts and is also a component of the nucleosome remodeling factor complex (NURF), a protein complex consisting of four polypeptides that facilitates the perturbation of chromatin structure in vitro in an ATP-dependent manner. This subunit may assist targeting of protein complexes to chromatin.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    p55/DCAF1 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair.

  • 細胞内局在

    Nuclear

  • 参照データベース

    • 別名

      • Caf 1 antibody
      • CAF1 p55 subunit antibody
      • chaf1b antibody
      • Chromatin assembly factor 1 p55 subunit antibody
      • Chromatin assembly factor 1 p60 subunit antibody
      • chromatin assembly factor 1 subunit antibody
      • dCAF1 antibody
      • NURF 55 antibody
      • p55 antibody
      • Probable histone binding protein Caf1 antibody
      see all

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献

    ab1766 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1766 は 4 報の論文で使用されています。

    • Abascal F  et al. Subfunctionalization via adaptive evolution influenced by genomic context: the case of histone chaperones ASF1a and ASF1b. Mol Biol Evol 30:1853-66 (2013). PubMed: 23645555
    • Lee H  et al. A dual role for the dREAM/MMB complex in the regulation of differentiation-specific E2F/RB target genes. Mol Cell Biol 32:2110-20 (2012). IP, WB ; Drosophila melanogaster . PubMed: 22451490
    • Lee H  et al. Drosophila RB proteins repress differentiation-specific genes via two different mechanisms. Mol Cell Biol 30:2563-77 (2010). WB ; Drosophila melanogaster . PubMed: 20176807
    • Groth A  et al. Human Asf1 regulates the flow of S phase histones during replicational stress. Mol Cell 17:301-11 (2005). PubMed: 15664198

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    ChIP abreview for Anti-p55/DCAF1 antibody

     Good
    Abreviews
    abreview image
    Application
    ChIP
    Detection step
    Real-time PCR
    Sample
    Fruit fly (Drosophila melanogaster) Cell lysate - nuclear (Kc167 cell line)
    Specification
    Kc167 cell line
    Negative control
    Coding sequences ChIP.
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Positive control
    Gene A enhancer region ChIP.
    Read More

    Dr. Kat Millen

    Verified customer

    投稿 Jul 25 2013

    Western blot abreview for Anti-p55/DCAF1 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Non-reduced Denaturing (4% gel)
    Sample
    Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (DmD8 and Kc167 cell lines)
    Specification
    DmD8 and Kc167 cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More

    Dr. Kat Millen

    Verified customer

    投稿 Jul 17 2013

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