アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

私たちはウェブサイトをできるだけ使いやすくするために、クッキーを使用しています。

クッキー・ポリシーでは、使用するクッキーをオプトアウトする方法について説明しています。

クッキーの設定を変更しないままでいる場合、このポリシーに同意しているとみなされます。

続ける 続ける

お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • 診断と治療のソリューション
    カスタム・ソリューション & パートナーシップ

    あなたの診断法や治療法を発展させるための、カスタム抗体開発およびコマーシャル・パートナーシップ

    私たちと共にカスタム・ソリューションを創造する

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  • ブログ
    アブカム・ブログ

    最新の情報はこちら.

    • キャンペーン
    • 製品情報
    • 技術情報
  1. Link

    p53-phospho-s15-antibody-ab1431.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Cell Cycle Cell Cycle Inhibitors p53
Share by email

Anti-p53 (phospho S15) 抗体 (ab1431)

  • Datasheet
  • SDS
Reviews (11)Q&A (8)References (98)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-p53 (phospho S15) antibody (ab1431)
  • Western blot - Anti-p53 (phospho S15) antibody (ab1431)
  • Immunocytochemistry - Anti-p53 (phospho S15) antibody (ab1431)
  • Immunocytochemistry - Anti-p53 (phospho S15) antibody (ab1431)
  • Immunocytochemistry - Anti-p53 (phospho S15) antibody (ab1431)

Key features and details

  • Rabbit polyclonal to p53 (phospho S15)
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
タンパク質
Product image
Recombinant Human p53 protein  (ab43615)

関連製品

製品の概要

  • 製品名

    Anti-p53 (phospho S15) antibody
    p53 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to p53 (phospho S15)
  • 由来種

    Rabbit
  • 特異性

    Recognizes endogenous levels of p53 (pS15) protein.
  • アプリケーション

    適用あり: ICC, WBmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human p53 (intracellular). Synthetic phospho-peptide surrounding amino acid Ser15 of human p53
    Database link: P04637

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 精製度

    Affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • p53
    • Cell Biology
    • Apoptosis
    • Intracellular
    • p53 Pathway
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • p53
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • p53
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • p53 pathway
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • p53 pathway

関連製品

  • Assay kits

    • p53 Transcription Factor Assay Kit (Colorimetric) (ab207225)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (ab157396)
  • Recombinant Protein

    • Recombinant Human p53 protein (ab43615)
  • Related Products

    • HeLa whole cell lysate (ab150035)

アプリケーション

Our Abpromise guarantee covers the use of ab1431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/100 - 1/500.
WB 1/500 - 1/1000. Predicted molecular weight: 53 kDa.

ターゲット情報

  • 機能

    Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.
  • 組織特異性

    Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.
  • 関連疾患

    Note=TP53 is found in increased amounts in a wide variety of transformed cells. TP53 is frequently mutated or inactivated in about 60% of cancers. TP53 defects are found in Barrett metaplasia a condition in which the normally stratified squamous epithelium of the lower esophagus is replaced by a metaplastic columnar epithelium. The condition develops as a complication in approximately 10% of patients with chronic gastroesophageal reflux disease and predisposes to the development of esophageal adenocarcinoma.
    Defects in TP53 are a cause of esophageal cancer (ESCR) [MIM:133239].
    Defects in TP53 are a cause of Li-Fraumeni syndrome (LFS) [MIM:151623]. LFS is an autosomal dominant familial cancer syndrome that in its classic form is defined by the existence of a proband affected by a sarcoma before 45 years with a first degree relative affected by any tumor before 45 years and another first degree relative with any tumor before 45 years or a sarcoma at any age. Other clinical definitions for LFS have been proposed (PubMed:8118819 and PubMed:8718514) and called Li-Fraumeni like syndrome (LFL). In these families affected relatives develop a diverse set of malignancies at unusually early ages. Four types of cancers account for 80% of tumors occurring in TP53 germline mutation carriers: breast cancers, soft tissue and bone sarcomas, brain tumors (astrocytomas) and adrenocortical carcinomas. Less frequent tumors include choroid plexus carcinoma or papilloma before the age of 15, rhabdomyosarcoma before the age of 5, leukemia, Wilms tumor, malignant phyllodes tumor, colorectal and gastric cancers.
    Defects in TP53 are involved in head and neck squamous cell carcinomas (HNSCC) [MIM:275355]; also known as squamous cell carcinoma of the head and neck.
    Defects in TP53 are a cause of lung cancer (LNCR) [MIM:211980].
    Defects in TP53 are a cause of choroid plexus papilloma (CPLPA) [MIM:260500]. Choroid plexus papilloma is a slow-growing benign tumor of the choroid plexus that often invades the leptomeninges. In children it is usually in a lateral ventricle but in adults it is more often in the fourth ventricle. Hydrocephalus is common, either from obstruction or from tumor secretion of cerebrospinal fluid. If it undergoes malignant transformation it is called a choroid plexus carcinoma. Primary choroid plexus tumors are rare and usually occur in early childhood.
    Defects in TP53 are a cause of adrenocortical carcinoma (ADCC) [MIM:202300]. ADCC is a rare childhood tumor of the adrenal cortex. It occurs with increased frequency in patients with the Beckwith-Wiedemann syndrome and is a component tumor in Li-Fraumeni syndrome.
  • 配列類似性

    Belongs to the p53 family.
  • ドメイン

    The nuclear export signal acts as a transcriptional repression domain. The TADI and TADII motifs (residues 17 to 25 and 48 to 56) correspond both to 9aaTAD motifs which are transactivation domains present in a large number of yeast and animal transcription factors.
  • 翻訳後修飾

    Acetylated. Acetylation of Lys-382 by CREBBP enhances transcriptional activity. Deacetylation of Lys-382 by SIRT1 impairs its ability to induce proapoptotic program and modulate cell senescence.
    Phosphorylation on Ser residues mediates transcriptional activation. Phosphorylated by HIPK1 (By similarity). Phosphorylation at Ser-9 by HIPK4 increases repression activity on BIRC5 promoter. Phosphorylated on Thr-18 by VRK1. Phosphorylated on Ser-20 by CHEK2 in response to DNA damage, which prevents ubiquitination by MDM2. Phosphorylated on Thr-55 by TAF1, which promotes MDM2-mediated degradation. Phosphorylated on Ser-46 by HIPK2 upon UV irradiation. Phosphorylation on Ser-46 is required for acetylation by CREBBP. Phosphorylated on Ser-392 following UV but not gamma irradiation. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated on Ser-15 upon ultraviolet irradiation; which is enhanced by interaction with BANP.
    Dephosphorylated by PP2A-PPP2R5C holoenzyme at Thr-55. SV40 small T antigen inhibits the dephosphorylation by the AC form of PP2A.
    May be O-glycosylated in the C-terminal basic region. Studied in EB-1 cell line.
    Ubiquitinated by MDM2 and SYVN1, which leads to proteasomal degradation. Ubiquitinated by RFWD3, which works in cooperation with MDM2 and may catalyze the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. Ubiquitinated by MKRN1 at Lys-291 and Lys-292, which leads to proteasomal degradation. Deubiquitinated by USP10, leading to its stabilization. Ubiquitinated by TRIM24, which leads to proteasomal degradation. Ubiquitination by TOPORS induces degradation. Deubiquitination by USP7, leading to stabilization. Isoform 4 is monoubiquitinated in an MDM2-independent manner.
    Monomethylated at Lys-372 by SETD7, leading to stabilization and increased transcriptional activation. Monomethylated at Lys-370 by SMYD2, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity. Lys-372 monomethylation prevents interaction with SMYD2 and subsequent monomethylation at Lys-370. Dimethylated at Lys-373 by EHMT1 and EHMT2. Monomethylated at Lys-382 by SETD8, promoting interaction with L3MBTL1 and leading to repress transcriptional activity. Demethylation of dimethylated Lys-370 by KDM1A prevents interaction with TP53BP1 and represses TP53-mediated transcriptional activation.
    Sumoylated by SUMO1.
  • 細胞内局在

    Cytoplasm; Cytoplasm. Nucleus. Nucleus > PML body. Endoplasmic reticulum. Interaction with BANP promotes nuclear localization. Recruited into PML bodies together with CHEK2; Nucleus. Cytoplasm. Localized in both nucleus and cytoplasm in most cells. In some cells, forms foci in the nucleus that are different from nucleoli; Nucleus. Cytoplasm. Localized in the nucleus in most cells but found in the cytoplasm in some cells; Nucleus. Cytoplasm. Localized mainly in the nucleus with minor staining in the cytoplasm; Nucleus. Cytoplasm. Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4 and Nucleus. Cytoplasm. Predominantly nuclear but translocates to the cytoplasm following cell stress.
  • Target information above from: UniProt accession P04637 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 7157 Human
    • Entrez Gene: 22059 Mouse
    • Entrez Gene: 24842 Rat
    • Omim: 191170 Human
    • SwissProt: P04637 Human
    • SwissProt: P02340 Mouse
    • SwissProt: P10361 Rat
    • Unigene: 654481 Human
    • Unigene: 222 Mouse
    • Unigene: 54443 Rat
    see all
  • 別名

    • Antigen NY-CO-13 antibody
    • BCC7 antibody
    • Cellular tumor antigen p53 antibody
    • FLJ92943 antibody
    • LFS1 antibody
    • Mutant tumor protein 53 antibody
    • p53 antibody
    • p53 tumor suppressor antibody
    • P53_HUMAN antibody
    • Phosphoprotein p53 antibody
    • Tp53 antibody
    • Transformation related protein 53 antibody
    • TRP53 antibody
    • tumor antigen p55 antibody
    • Tumor protein 53 antibody
    • Tumor protein p53 antibody
    • Tumor suppressor p53 antibody
    see all

画像

  • Western blot - Anti-p53 (phospho S15) antibody (ab1431)
    Western blot - Anti-p53 (phospho S15) antibody (ab1431)
    All lanes : Anti-p53 (phospho S15) antibody (ab1431) at 1 µg/ml

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells at 20 µg
    Lane 2 : HEK-293T cells incubated with etoposide at 20 µg
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) cells at 20 µg
    Lane 4 : MCF7 cells incubated 6 hours with camptothecin at 20 µg
    Lane 5 : MCF7 cells incubated 16 hours with camptothecin at 20 µg
    Lane 6 : MCF7 cells incubated 24 hours with camptothecin at 20 µg
    Lane 7 : HEK-293T incubated with etoposide at 7.5 µg
    Lane 8 : Lambda phosphatase (400 times-diluted)-treated extract of HEK-293T incubated with etoposide at 7.5 µg
    Lane 9 : Lambda phosphatase (100 times-diluted)-treated extract of HEK-293T incubated with etoposide at 7.5 µg
    Lane 10 : Lambda phosphatase (25 times-diluted)-treated extract of HEK-293T incubated with etoposide at 7.5 µg

    Secondary
    All lanes : Goat anti rabbit IgG(H&L)-HRP at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa


    Exposure time: 2 minutes


    SDS PAGE performed under reducing conditions (100mM DTT, Sample heated at 50°C).

    Blocking: 5% Milk + PBS for 3 hours at RT.

    Primary antibody: 5% BSA + PBS overnight at 4 C.

    Secondary antibody: 5% Milk + PBS for 2 hour at RT.

     

  • Western blot - Anti-p53 (phospho S15) antibody (ab1431)
    Western blot - Anti-p53 (phospho S15) antibody (ab1431)
    All lanes : Anti-p53 (phospho S15) antibody (ab1431)

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (ab157396)

    Predicted band size: 53 kDa

  • Immunocytochemistry - Anti-p53 (phospho S15) antibody (ab1431)
    Immunocytochemistry - Anti-p53 (phospho S15) antibody (ab1431)This image is a courtesy of Anonymous Abreview

    ab1431 staining p53 in rat bone marrow cells by Immunocytochemistry/ Immunofluorescence.

    The cells were fixed in paraformaldehyde, permeabilized in 0.1% Triton X-100 and then blocked using 5% BSA for 1 hour at 25°C. Samples were then incubated with primary antibody at 1:100 for 9 hours at 4°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (green) ab150077) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).

    These pictures were taken in the different fields of the same bone marrow cell slide.

    See Abreview

  • Immunocytochemistry - Anti-p53 (phospho S15) antibody (ab1431)
    Immunocytochemistry - Anti-p53 (phospho S15) antibody (ab1431)
    Immunocytochemistry analysis of PC12 cells labeling p53 (phospho S15) with ab1431.

     

  • Immunocytochemistry - Anti-p53 (phospho S15) antibody (ab1431)
    Immunocytochemistry - Anti-p53 (phospho S15) antibody (ab1431)Image courtesy of an anonymous Abreview

    ab1431 staining p53 in mouse bone marrow WBCs cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 2 hours at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

プロトコール

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (98)

    ab1431 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1431 は 98 報の論文で使用されています。

    • You M  et al. 10-Hydroxydecanoic acid inhibits LPS-induced inflammation by targeting p53 in microglial cells. Int Immunopharmacol 84:106501 (2020). PubMed: 32311670
    • Zhang C  et al. FOXO4-DRI alleviates age-related testosterone secretion insufficiency by targeting senescent Leydig cells in aged mice. Aging (Albany NY) 12:1272-1284 (2020). PubMed: 31959736
    • Liu YH  et al. Renoprotective Effects of a New Free Radical Scavenger, XH-003, against Cisplatin-Induced Nephrotoxicity. Oxid Med Cell Longev 2020:9820168 (2020). PubMed: 32377314
    • Takahashi K  et al. Heterogenous Nuclear Ribonucleoprotein H1 Promotes Colorectal Cancer Progression through the Stabilization of mRNA of Sphingosine-1-Phosphate Lyase 1. Int J Mol Sci 21:N/A (2020). PubMed: 32630435
    • Kita A  et al. Probiotic-derived ferrichrome inhibits the growth of refractory pancreatic cancer cells. Int J Oncol 57:721-732 (2020). PubMed: 32705165
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 19 Abreviews or Q&A

    Western blot abreview for Anti-p53 (phospho S15) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Liver)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (10)
    Loading amount
    30 µg
    Treatment
    200µM Palmitate
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 06 2020

    Immunocytochemistry abreview for Anti-p53 (phospho S15) antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    投稿 Jul 03 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-p53 (phospho S15) antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (HF)
    Permeabilization
    Yes - PBS-TWEEN 0.05%
    Specification
    HF
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 28 2018

    Western blot abreview for Anti-p53 (phospho S15) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Gradient Gel)
    Loading amount
    20 µg
    Treatment
    10 uM Camptothecin (CPT) for 1hr
    Specification
    U2OS cells
    Blocking step
    Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 23 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-p53 (phospho S15) antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% TritonX100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    投稿 Aug 12 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-p53 (phospho S15) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (fetal lung)
    Specification
    fetal lung
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - PBST for 1 hour
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 1.5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 16 2013

    Question

    Catalog number(s): 1431

    Product(s): Rabbit polyclonal to p53 (phospho S15)

    Survey results
    ----------------------------------------------------------------------------------
    Telephone section
    Q. Did you contact Abcam's Scientific Support by telephone in reference to this complaint?
    A. Yes
    Q. Was it clear from the phone options which number you needed to press to get your issue resolved?
    A. Yes

    Protocol advice section
    Q. Did Abcam's Scientific Support give you protocol advice?
    A.Yes
    Q. How did you feel about being given protocol advice?
    A.I requested protocol advice
    Q. Did the product work successfully after following the advice?
    A.No
    Q. Why have you not tried the protocol advice? (Select all that apply.)
    A.

    Free of charge replacement section
    Q. Did Abcam's Scientific Support give you a free of charge replacement?
    A. Yes

    Q. Did the replacement product work successfully?
    A.No
    Credit note / refund section
    Q. Did Abcam's Scientific Support give you a credit note or a refund?
    A.Yes

    Q. Would you have preferred a different outcome from receiving a credit note or refund?
    A. Availability of a second Ab choice for mouse tissue
    Overall satisfaction section
    Q. How satisfied are you with how the complaint was handled?
    A.Very satisfied
    Q. Could we have done anything to better resolve the problem?
    A.The tech was great with helping me trouble shoot possible issues.
    Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution?
    A.No, I would not mind
    Q. Please share with us any final thoughts you may have about Abcam, the complaints process, or our products.
    A.

    Read More

    Abcam community

    Verified customer

    Asked on May 10 2012

    Answer

    Thank you for your participation in Abcam's post complaint survey.

    I am glad to hear that your satisfied with our service. Although the outcome of a working antibody is always prefered,unfortunately, we needed to refund you purchase as the free of charge replacement did not work either.

    You mentioned in your response "Availability of a second Ab choice for mouse tissue" as a prefered outcome. Could you please briefly explain what you specifically refering to. We do have a large selection of secondary antibodies, but maybe not what you needed. Please let me know and I can look into this further.

    If there is anything else I can do for you, or if you have questions to any of our products (e.g. primary antibodies, lysates, secondary antibodies, kits, controls, gels, proteins/peptides, slides, biochemicals, conjugation kits), please let me know.

    Read More

    Abcam Scientific Support

    Answered on May 10 2012

    Question

    At the moment I am trying to investigate the p53 activity in my cell line using the western blot technique. I am using a antibody for total p53 and a phospho-p53 (Ser15) antibody. I ordered those antibodies from a different company. However I am not completely happy with those antibodies. Therefore I am looking into ordering new antibodies.

    I was wondering which are the conditions you optimised your p53 antibody (ab26 or ab28) and phospho-p53 (Ser15) antibody (amount of protein needed, Blotiing solution and time, etc.)? How long would the shipping be taking?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 16 2012

    Answer

    Thank you for contacting us.

    Our p53 antibody ab26 has been optimized by loading 20 ug per lane of HeLa cells, using 5% BSA as the blocking agent for 1 hour at room temperature, and incubating with the primary antibody at a concentration of 5ug / mL overnight at 4C. To view the protocols used by our customers, you can browse our WB Abreviews for this product:

    https://www.abcam.com/p53-antibody-PAb-240-ab26-reviews.html#WB

    Ab28 and ab1431 can be used with a similar protocol, loading 20-40 ug mer lane, using a 5% milk block, and a primary antibody dilution of 1:1000.

    We will ship anywhere in the UK or US overnight for next day delivery. I hope this helps, please let me know if you need any additional information or assistance.

    Read More

    Abcam Scientific Support

    Answered on Mar 16 2012

    Question

    IHC-P with mouse testis
    previous lot in 2010 worked great
    recent lot did not, received free of charge replacement
    replacement lot (GR38575-5) not working either: no staining
    Ab: 1/5, 1/10, 1/20
    block: goat serum
    AR: citrate, EDTA
    secondary: Alexa, works well
    checked previous tissue with new lot - not working

    Read More

    Abcam community

    Verified customer

    Asked on Mar 14 2012

    Answer

    Thank you for contacting us.

    Your credit note ID is xxx.

    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

    The credit note ID is for your reference only and does not automatically guarantee the credit.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

    Read More

    Abcam Scientific Support

    Answered on Mar 14 2012

    Question

    I would like a Certificate of analysis for:  ab1431

    Read More

    Abcam community

    Verified customer

    Asked on Nov 09 2011

    Answer

    Thank you for contacting Abcam. Attached please find the certificate of compliance you requested. I hope this information is helpful.  Please do not hesitate to contact us if you have any additional questions.

    Read More

    Abcam Scientific Support

    Answered on Nov 09 2011

    1-10 of 19 Abreviews or Q&A

    •  戻る
    • 1
    • 2
    • 次へ 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    ブログ

    ブログを読む

    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2021 Abcam plc. All rights reserved.