Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Sodium Citrate
Sample
Mouse Tissue sections (mouse brain cerebellum)
Specification
mouse brain cerebellum
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/250
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse 2nd Ab
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/500
Additional data
Additional Notes
p53 was stained in a mouse model of medulloblastoma. The picture shows the strong staining in the center of the tumor.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 23 2015