Application
Western blot
Sample
Rabbit Tissue lysate - whole (Brain)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
0.2 µg
Treatment
hypoxic 40 minutes at E25
Specification
Brain
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
30 minute(s) · Temperature: 22°C · Diluent: Antibody dilution buffer
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Luminol-S + Peroxide
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 53 kDa Non-specific: 47, 60, 69, 93, 125 kDa
Positive control
NA
Additional data
Additional Notes
Lane 1: ladders; Lane 2: rabbit cortex tissue lysate.
This WB was done in WES (proteinsimple) based on HPLC-like method using capillaries, which hold less than 0.5 ul volume for each sample. The amount of protein is reduced to 400ng/ul.
Abcam response
Thank you for your valuable feedback. ab26 has not been previously tested in rabbit sample. It is possible that this antibody may not be suitable for use in this species. We welcome other Abreviews to determine this.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jul 20 2018