Application
Western blot
Sample
Rat Cell lysate - other (Macrophages)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
Macrophages
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST + 2.5% milk
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Goat anti rmouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
Fractions (mitochondria and cytosol) not containing ER
Exposure
5 second(s)
Bands
Specific: 59 kDa
Additional data
Additional Notes
Only microsomal fraction (containing ER) was positive for the ER marker
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 08 2018